Academic Journal

Pseudoneobacillus rhizolycopersici sp. nov., a novel multifunctional rhizobacterium for the biocontrol of tomato bacterial wilt.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος: Pseudoneobacillus rhizolycopersici sp. nov., a novel multifunctional rhizobacterium for the biocontrol of tomato bacterial wilt.
Συγγραφείς: Pan S; Key Laboratory of Agricultural Microbiomics and Precision Application (MARA), Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Key Laboratory of Agricultural Microbiome (MARA), State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, PR China.; Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, PR China.; Guangdong Provincial Observation and Research Station for Microbial Science of the Ecosystems of Origin for Geographical Indication Agricultural Products, Guangdong, PR China., Feng Z; Key Laboratory of Agricultural Microbiomics and Precision Application (MARA), Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Key Laboratory of Agricultural Microbiome (MARA), State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, PR China.; Guangdong Provincial Observation and Research Station for Microbial Science of the Ecosystems of Origin for Geographical Indication Agricultural Products, Guangdong, PR China., Chen M; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Guangdong Engineering Research Center for Litchi, College of Horticulture, South China Agricultural University, Guangzhou, 510642, PR China., Xie X; Key Laboratory of Agricultural Microbiomics and Precision Application (MARA), Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Key Laboratory of Agricultural Microbiome (MARA), State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, PR China.; Guangdong Provincial Observation and Research Station for Microbial Science of the Ecosystems of Origin for Geographical Indication Agricultural Products, Guangdong, PR China., Yao Q; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Guangdong Engineering Research Center for Litchi, College of Horticulture, South China Agricultural University, Guangzhou, 510642, PR China., Deng MR; Key Laboratory of Agricultural Microbiomics and Precision Application (MARA), Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Key Laboratory of Agricultural Microbiome (MARA), State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, PR China.; Guangdong Provincial Observation and Research Station for Microbial Science of the Ecosystems of Origin for Geographical Indication Agricultural Products, Guangdong, PR China., Zhu H; Key Laboratory of Agricultural Microbiomics and Precision Application (MARA), Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Key Laboratory of Agricultural Microbiome (MARA), State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, PR China.; Guangdong Provincial Observation and Research Station for Microbial Science of the Ecosystems of Origin for Geographical Indication Agricultural Products, Guangdong, PR China.
Πηγή: International journal of systematic and evolutionary microbiology [Int J Syst Evol Microbiol] 2026 Aug; Vol. 76 (8).
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Microbiology Society Country of Publication: England NLM ID: 100899600 Publication Model: Print Cited Medium: Internet ISSN: 1466-5034 (Electronic) Linking ISSN: 14665026 NLM ISO Abbreviation: Int J Syst Evol Microbiol Subsets: MEDLINE
Imprint Name(s): Publication: 2015- : London : Microbiology Society
Original Publication: Reading, UK : Society for General Microbiology, c2000-
Ιατρικοί όροι (MeSH): Solanum lycopersicum*/microbiology , Plant Diseases*/microbiology , Plant Diseases*/prevention & control , Bacillaceae*/classification , Bacillaceae*/genetics , Bacillaceae*/isolation & purification , Phylogeny* , Soil Microbiology*, RNA, Ribosomal, 16S/genetics ; Fatty Acids/chemistry ; DNA, Bacterial/genetics ; Vitamin K 2/analogs & derivatives ; Vitamin K 2/chemistry ; Base Composition ; Bacterial Typing Techniques ; Rhizosphere ; Sequence Analysis, DNA ; Nucleic Acid Hybridization
Περίληψη: A novel Gram-stain-positive, rod-shaped bacterium with peritrichous flagella, designated C159T, was isolated from tomato rhizosphere soil. Growth of strain C159T occurred at 4-45 °C, pH 6.0-10.0 and in the presence of 0-5% NaCl. Based on 16S rRNA gene sequence analysis, strain C159T belongs to the genus Pseudoneobacillus and has a sequence similarity of 98.70% with Pseudoneobacillus rhizosphaerae JJ-79T. The whole genome of strain C159T was 4.65 Mb, with a DNA G+C content of 38.65 mol%. The average nucleotide identity between strain C159T and P. rhizosphaerae JJ-79T was 75.31%, and the digital DNA-DNA hybridization value was 19.6%, supporting its designation as a novel species of the genus Pseudoneobacillus. The major respiratory quinone of strain C159T was menaquinone 7; the major fatty acids were iso-C14:0, iso-C15:0 and anteiso-C15:0; and the major polar lipids are diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Based on its phenotypic, chemotaxonomic, phylogenetic and genomic characteristics, strain C159T represents a novel species in the genus Pseudoneobacillus, for which the name Pseudoneobacillus rhizolycopersici sp. nov. is proposed. The type strain is C159T (=GDMCC 1.5552T=KCTC 43820T). In addition, strain C159T possesses the ability to produce indole-3-acetic acid (33.72 mg·l-1) and to mineralize insoluble organic phosphorus. In a pot experiment, this strain demonstrated a plant growth-promoting effect of 22.68-44.49% and a disease suppression efficacy of 89.29% against bacterial wilt of tomato caused by Ralstonia solanacearum. The above results confirm that strain C159T is a novel multifunctional rhizobacterium for the biocontrol of tomato bacterial wilt.
Contributed Indexing: Keywords: Pseudoneobacillus; bacterial wilt; growth promotion; rhizosphere soil
Substance Nomenclature: 0 (RNA, Ribosomal, 16S)
0 (Fatty Acids)
0 (DNA, Bacterial)
11032-49-8 (Vitamin K 2)
8427BML8NY (menaquinone 7)
Entry Date(s): Date Created: 20260819 Date Completed: 20260819 Latest Revision: 20260819
Update Code: 20260820
DOI: 10.1099/ijsem.0.007272
PMID: 42616563
Βάση Δεδομένων: MEDLINE