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Pseudoneobacillus rhizolycopersici sp. nov., a novel multifunctional rhizobacterium for the biocontrol of tomato bacterial wilt.

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Τίτλος: Pseudoneobacillus rhizolycopersici sp. nov., a novel multifunctional rhizobacterium for the biocontrol of tomato bacterial wilt.
Συγγραφείς: Pan S; Key Laboratory of Agricultural Microbiomics and Precision Application (MARA), Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Key Laboratory of Agricultural Microbiome (MARA), State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, PR China.; Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, PR China.; Guangdong Provincial Observation and Research Station for Microbial Science of the Ecosystems of Origin for Geographical Indication Agricultural Products, Guangdong, PR China., Feng Z; Key Laboratory of Agricultural Microbiomics and Precision Application (MARA), Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Key Laboratory of Agricultural Microbiome (MARA), State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, PR China.; Guangdong Provincial Observation and Research Station for Microbial Science of the Ecosystems of Origin for Geographical Indication Agricultural Products, Guangdong, PR China., Chen M; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Guangdong Engineering Research Center for Litchi, College of Horticulture, South China Agricultural University, Guangzhou, 510642, PR China., Xie X; Key Laboratory of Agricultural Microbiomics and Precision Application (MARA), Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Key Laboratory of Agricultural Microbiome (MARA), State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, PR China.; Guangdong Provincial Observation and Research Station for Microbial Science of the Ecosystems of Origin for Geographical Indication Agricultural Products, Guangdong, PR China., Yao Q; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Guangdong Engineering Research Center for Litchi, College of Horticulture, South China Agricultural University, Guangzhou, 510642, PR China., Deng MR; Key Laboratory of Agricultural Microbiomics and Precision Application (MARA), Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Key Laboratory of Agricultural Microbiome (MARA), State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, PR China.; Guangdong Provincial Observation and Research Station for Microbial Science of the Ecosystems of Origin for Geographical Indication Agricultural Products, Guangdong, PR China., Zhu H; Key Laboratory of Agricultural Microbiomics and Precision Application (MARA), Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Key Laboratory of Agricultural Microbiome (MARA), State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, PR China.; Guangdong Provincial Observation and Research Station for Microbial Science of the Ecosystems of Origin for Geographical Indication Agricultural Products, Guangdong, PR China.
Πηγή: International journal of systematic and evolutionary microbiology [Int J Syst Evol Microbiol] 2026 Aug; Vol. 76 (8).
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Microbiology Society Country of Publication: England NLM ID: 100899600 Publication Model: Print Cited Medium: Internet ISSN: 1466-5034 (Electronic) Linking ISSN: 14665026 NLM ISO Abbreviation: Int J Syst Evol Microbiol Subsets: MEDLINE
Imprint Name(s): Publication: 2015- : London : Microbiology Society
Original Publication: Reading, UK : Society for General Microbiology, c2000-
Ιατρικοί όροι (MeSH): Solanum lycopersicum*/microbiology , Plant Diseases*/microbiology , Plant Diseases*/prevention & control , Bacillaceae*/classification , Bacillaceae*/genetics , Bacillaceae*/isolation & purification , Phylogeny* , Soil Microbiology*, RNA, Ribosomal, 16S/genetics ; Fatty Acids/chemistry ; DNA, Bacterial/genetics ; Vitamin K 2/analogs & derivatives ; Vitamin K 2/chemistry ; Base Composition ; Bacterial Typing Techniques ; Rhizosphere ; Sequence Analysis, DNA ; Nucleic Acid Hybridization
Περίληψη: A novel Gram-stain-positive, rod-shaped bacterium with peritrichous flagella, designated C159T, was isolated from tomato rhizosphere soil. Growth of strain C159T occurred at 4-45 °C, pH 6.0-10.0 and in the presence of 0-5% NaCl. Based on 16S rRNA gene sequence analysis, strain C159T belongs to the genus Pseudoneobacillus and has a sequence similarity of 98.70% with Pseudoneobacillus rhizosphaerae JJ-79T. The whole genome of strain C159T was 4.65 Mb, with a DNA G+C content of 38.65 mol%. The average nucleotide identity between strain C159T and P. rhizosphaerae JJ-79T was 75.31%, and the digital DNA-DNA hybridization value was 19.6%, supporting its designation as a novel species of the genus Pseudoneobacillus. The major respiratory quinone of strain C159T was menaquinone 7; the major fatty acids were iso-C14:0, iso-C15:0 and anteiso-C15:0; and the major polar lipids are diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Based on its phenotypic, chemotaxonomic, phylogenetic and genomic characteristics, strain C159T represents a novel species in the genus Pseudoneobacillus, for which the name Pseudoneobacillus rhizolycopersici sp. nov. is proposed. The type strain is C159T (=GDMCC 1.5552T=KCTC 43820T). In addition, strain C159T possesses the ability to produce indole-3-acetic acid (33.72 mg·l-1) and to mineralize insoluble organic phosphorus. In a pot experiment, this strain demonstrated a plant growth-promoting effect of 22.68-44.49% and a disease suppression efficacy of 89.29% against bacterial wilt of tomato caused by Ralstonia solanacearum. The above results confirm that strain C159T is a novel multifunctional rhizobacterium for the biocontrol of tomato bacterial wilt.
Contributed Indexing: Keywords: Pseudoneobacillus; bacterial wilt; growth promotion; rhizosphere soil
Substance Nomenclature: 0 (RNA, Ribosomal, 16S)
0 (Fatty Acids)
0 (DNA, Bacterial)
11032-49-8 (Vitamin K 2)
8427BML8NY (menaquinone 7)
Entry Date(s): Date Created: 20260819 Date Completed: 20260819 Latest Revision: 20260819
Update Code: 20260820
DOI: 10.1099/ijsem.0.007272
PMID: 42616563
Βάση Δεδομένων: MEDLINE
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DbLabel: MEDLINE
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PubType: Academic Journal
PubTypeId: academicJournal
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  Label: Title
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  Data: Pseudoneobacillus rhizolycopersici sp. nov., a novel multifunctional rhizobacterium for the biocontrol of tomato bacterial wilt.
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  Label: Authors
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  Data: <searchLink fieldCode="AU" term="%22Pan+S%22">Pan S</searchLink>; Key Laboratory of Agricultural Microbiomics and Precision Application (MARA), Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Key Laboratory of Agricultural Microbiome (MARA), State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, PR China.; Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, PR China.; Guangdong Provincial Observation and Research Station for Microbial Science of the Ecosystems of Origin for Geographical Indication Agricultural Products, Guangdong, PR China.<br /><searchLink fieldCode="AU" term="%22Feng+Z%22">Feng Z</searchLink>; Key Laboratory of Agricultural Microbiomics and Precision Application (MARA), Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Key Laboratory of Agricultural Microbiome (MARA), State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, PR China.; Guangdong Provincial Observation and Research Station for Microbial Science of the Ecosystems of Origin for Geographical Indication Agricultural Products, Guangdong, PR China.<br /><searchLink fieldCode="AU" term="%22Chen+M%22">Chen M</searchLink>; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Guangdong Engineering Research Center for Litchi, College of Horticulture, South China Agricultural University, Guangzhou, 510642, PR China.<br /><searchLink fieldCode="AU" term="%22Xie+X%22">Xie X</searchLink>; Key Laboratory of Agricultural Microbiomics and Precision Application (MARA), Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Key Laboratory of Agricultural Microbiome (MARA), State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, PR China.; Guangdong Provincial Observation and Research Station for Microbial Science of the Ecosystems of Origin for Geographical Indication Agricultural Products, Guangdong, PR China.<br /><searchLink fieldCode="AU" term="%22Yao+Q%22">Yao Q</searchLink>; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Guangdong Engineering Research Center for Litchi, College of Horticulture, South China Agricultural University, Guangzhou, 510642, PR China.<br /><searchLink fieldCode="AU" term="%22Deng+MR%22">Deng MR</searchLink>; Key Laboratory of Agricultural Microbiomics and Precision Application (MARA), Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Key Laboratory of Agricultural Microbiome (MARA), State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, PR China.; Guangdong Provincial Observation and Research Station for Microbial Science of the Ecosystems of Origin for Geographical Indication Agricultural Products, Guangdong, PR China.<br /><searchLink fieldCode="AU" term="%22Zhu+H%22">Zhu H</searchLink>; Key Laboratory of Agricultural Microbiomics and Precision Application (MARA), Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Key Laboratory of Agricultural Microbiome (MARA), State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, PR China.; Guangdong Provincial Observation and Research Station for Microbial Science of the Ecosystems of Origin for Geographical Indication Agricultural Products, Guangdong, PR China.
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  Data: <searchLink fieldCode="JN" term="%22100899600%22">International journal of systematic and evolutionary microbiology</searchLink> [Int J Syst Evol Microbiol] 2026 Aug; Vol. 76 (8).
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  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22Microbiology+Society%22">Microbiology Society </searchLink><i>Country of Publication: </i>England <i>NLM ID: </i>100899600 <i>Publication Model: </i>Print <i>Cited Medium: </i>Internet <i>ISSN: </i>1466-5034 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2214665026%22">14665026 </searchLink><i>NLM ISO Abbreviation: </i>Int J Syst Evol Microbiol <i>Subsets: </i>MEDLINE
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  Data: <i>Publication</i>: 2015- : London : Microbiology Society<br /><i>Original Publication</i>: Reading, UK : Society for General Microbiology, c2000-
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  Data: <searchLink fieldCode="MM" term="%22Solanum+lycopersicum%22">Solanum lycopersicum*</searchLink>/<searchLink fieldCode="MM" term="%22Solanum+lycopersicum+microbiology%22">microbiology</searchLink> <br /><searchLink fieldCode="MM" term="%22Plant+Diseases%22">Plant Diseases*</searchLink>/<searchLink fieldCode="MM" term="%22Plant+Diseases+microbiology%22">microbiology</searchLink> <br /><searchLink fieldCode="MM" term="%22Plant+Diseases%22">Plant Diseases*</searchLink>/<searchLink fieldCode="MM" term="%22Plant+Diseases+prevention+%26+control%22">prevention & control</searchLink> <br /><searchLink fieldCode="MM" term="%22Bacillaceae%22">Bacillaceae*</searchLink>/<searchLink fieldCode="MM" term="%22Bacillaceae+classification%22">classification</searchLink> <br /><searchLink fieldCode="MM" term="%22Bacillaceae%22">Bacillaceae*</searchLink>/<searchLink fieldCode="MM" term="%22Bacillaceae+genetics%22">genetics</searchLink> <br /><searchLink fieldCode="MM" term="%22Bacillaceae%22">Bacillaceae*</searchLink>/<searchLink fieldCode="MM" term="%22Bacillaceae+isolation+%26+purification%22">isolation & purification</searchLink> <br /><searchLink fieldCode="MM" term="%22Phylogeny%22">Phylogeny*</searchLink> <br /><searchLink fieldCode="MM" term="%22Soil+Microbiology%22">Soil Microbiology*</searchLink><br /><searchLink fieldCode="MH" term="%22RNA%2C+Ribosomal%2C+16S%22">RNA, Ribosomal, 16S</searchLink>/<searchLink fieldCode="MH" term="%22RNA%2C+Ribosomal%2C+16S+genetics%22">genetics</searchLink> ; <searchLink fieldCode="MH" term="%22Fatty+Acids%22">Fatty Acids</searchLink>/<searchLink fieldCode="MH" term="%22Fatty+Acids+chemistry%22">chemistry</searchLink> ; <searchLink fieldCode="MH" term="%22DNA%2C+Bacterial%22">DNA, Bacterial</searchLink>/<searchLink fieldCode="MH" term="%22DNA%2C+Bacterial+genetics%22">genetics</searchLink> ; <searchLink fieldCode="MH" term="%22Vitamin+K+2%22">Vitamin K 2</searchLink>/<searchLink fieldCode="MH" term="%22Vitamin+K+2+analogs+%26+derivatives%22">analogs & derivatives</searchLink> ; <searchLink fieldCode="MH" term="%22Vitamin+K+2%22">Vitamin K 2</searchLink>/<searchLink fieldCode="MH" term="%22Vitamin+K+2+chemistry%22">chemistry</searchLink> ; <searchLink fieldCode="MH" term="%22Base+Composition%22">Base Composition</searchLink> ; <searchLink fieldCode="MH" term="%22Bacterial+Typing+Techniques%22">Bacterial Typing Techniques</searchLink> ; <searchLink fieldCode="MH" term="%22Rhizosphere%22">Rhizosphere</searchLink> ; <searchLink fieldCode="MH" term="%22Sequence+Analysis%2C+DNA%22">Sequence Analysis, DNA</searchLink> ; <searchLink fieldCode="MH" term="%22Nucleic+Acid+Hybridization%22">Nucleic Acid Hybridization</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: A novel Gram-stain-positive, rod-shaped bacterium with peritrichous flagella, designated C159<superscript>T</superscript>, was isolated from tomato rhizosphere soil. Growth of strain C159<superscript>T</superscript> occurred at 4-45 °C, pH 6.0-10.0 and in the presence of 0-5% NaCl. Based on 16S rRNA gene sequence analysis, strain C159<superscript>T</superscript> belongs to the genus Pseudoneobacillus and has a sequence similarity of 98.70% with Pseudoneobacillus rhizosphaerae JJ-79<superscript>T</superscript>. The whole genome of strain C159<superscript>T</superscript> was 4.65 Mb, with a DNA G+C content of 38.65 mol%. The average nucleotide identity between strain C159<superscript>T</superscript> and P. rhizosphaerae JJ-79<superscript>T</superscript> was 75.31%, and the digital DNA-DNA hybridization value was 19.6%, supporting its designation as a novel species of the genus Pseudoneobacillus. The major respiratory quinone of strain C159<superscript>T</superscript> was menaquinone 7; the major fatty acids were iso-C<subscript>14:0</subscript>, iso-C<subscript>15:0</subscript> and anteiso-C<subscript>15:0</subscript>; and the major polar lipids are diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Based on its phenotypic, chemotaxonomic, phylogenetic and genomic characteristics, strain C159<superscript>T</superscript> represents a novel species in the genus Pseudoneobacillus, for which the name Pseudoneobacillus rhizolycopersici sp. nov. is proposed. The type strain is C159<superscript>T</superscript> (=GDMCC 1.5552<superscript>T</superscript>=KCTC 43820<superscript>T</superscript>). In addition, strain C159<superscript>T</superscript> possesses the ability to produce indole-3-acetic acid (33.72 mg·l<superscript>-1</superscript>) and to mineralize insoluble organic phosphorus. In a pot experiment, this strain demonstrated a plant growth-promoting effect of 22.68-44.49% and a disease suppression efficacy of 89.29% against bacterial wilt of tomato caused by Ralstonia solanacearum. The above results confirm that strain C159<superscript>T</superscript> is a novel multifunctional rhizobacterium for the biocontrol of tomato bacterial wilt.
– Name: SubjectMinor
  Label: Contributed Indexing
  Group:
  Data: <i>Keywords: </i>Pseudoneobacillus; bacterial wilt; growth promotion; rhizosphere soil
– Name: NumberCAS
  Label: Substance Nomenclature
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  Data: 0 (RNA, Ribosomal, 16S)<br />0 (Fatty Acids)<br />0 (DNA, Bacterial)<br />11032-49-8 (Vitamin K 2)<br />8427BML8NY (menaquinone 7)
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  Data: <i>Date Created: </i>20260819 <i>Date Completed: </i>20260819 <i>Latest Revision: </i>20260819
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  Data: 20260820
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  Data: 10.1099/ijsem.0.007272
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  Data: 42616563
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=cmedm&AN=42616563
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        Value: 10.1099/ijsem.0.007272
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      – Code: eng
        Text: English
    Subjects:
      – SubjectFull: RNA, Ribosomal, 16S genetics
        Type: general
      – SubjectFull: Fatty Acids chemistry
        Type: general
      – SubjectFull: DNA, Bacterial genetics
        Type: general
      – SubjectFull: Vitamin K 2 analogs & derivatives
        Type: general
      – SubjectFull: Vitamin K 2 chemistry
        Type: general
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      – SubjectFull: Nucleic Acid Hybridization
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      – SubjectFull: Solanum lycopersicum microbiology
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      – SubjectFull: Plant Diseases prevention & control
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      – TitleFull: Pseudoneobacillus rhizolycopersici sp. nov., a novel multifunctional rhizobacterium for the biocontrol of tomato bacterial wilt.
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              Text: 2026 Aug
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