Academic Journal
View in EDS

Single-cell transcriptomics and functional analyses identified SPIN.DOC as a regulator for Wnt signaling and cancer stemness in colorectal cancer.

Bibliographic Details
Title: Single-cell transcriptomics and functional analyses identified SPIN.DOC as a regulator for Wnt signaling and cancer stemness in colorectal cancer.
Authors: Devi KM; Department of Biotechnology, Manipur University, Imphal, 795003, India., Abbas E; Department of Molecular Biology and Biotechnology, Tezpur University, Napaam, Tezpur, 784028, Assam, India., Singh TD; Department of Biotechnology, Manipur University, Imphal, 795003, India., Deka R; Department of Molecular Biology and Biotechnology, Tezpur University, Napaam, Tezpur, 784028, Assam, India., Kumar A; Department of Molecular Biology and Biotechnology, Tezpur University, Napaam, Tezpur, 784028, Assam, India., Singh LS; Department of Biotechnology, Manipur University, Imphal, 795003, India., Singh TR; Department of Molecular Biology and Biotechnology, Tezpur University, Napaam, Tezpur, 784028, Assam, India. rthiyam@tezu.ernet.in.
Source: Human cell [Hum Cell] 2026 Aug 19; Vol. 39 (9). Date of Electronic Publication: 2026 Aug 19.
Publication Type: Journal Article
Language: English
Journal Info: Publisher: Springer Country of Publication: Japan NLM ID: 8912329 Publication Model: Electronic Cited Medium: Internet ISSN: 1749-0774 (Electronic) Linking ISSN: 09147470 NLM ISO Abbreviation: Hum Cell Subsets: MEDLINE
Imprint Name(s): Publication: 2011- : Tokyo : Springer
Original Publication: Tōkyō-to : Hito Saibō Kenkyūkai : Kanishobo, Shōwa 63-nen [1988]-
MeSH Terms: Wnt Signaling Pathway*/genetics , Wnt Signaling Pathway*/physiology , Colorectal Neoplasms*/genetics , Colorectal Neoplasms*/pathology , Neoplastic Stem Cells*/pathology , Single-Cell Analysis*/methods , Gene Expression*/genetics , Carcinogenesis*/genetics , Transcriptome*/genetics, Epithelial-Mesenchymal Transition/genetics ; Cell Proliferation/genetics ; beta Catenin/metabolism ; beta Catenin/genetics ; Gene Expression Regulation, Neoplastic/genetics ; Humans ; Single-Cell Gene Expression Analysis ; Cell Line, Tumor ; Disease Progression
Abstract: The role of SPIN.DOC in tumorigenesis remains unclear. In this study, we utilized single-cell RNA sequencing (scRNA-seq) data to investigate SPIN.DOC expression in a cohort of normal, primary human colorectal cancer (CRC) and metastatic tissues from CRC patients. Our findings revealed differential expressions of SPIN.DOC across multiple cell clusters in different conditions and with the highest expression observed in tumor samples, suggesting its potential role in CRC progression. Further analysis showed that SPIN.DOC positive cell-cluster showed upregulation of β-catenin and cancer stem cell (CSC) marker genes, indicating its role in activating Wnt signaling for cell proliferation. Additionally, these cells also showed downregulation of genes associated with cell-cell junctions, promoting epithelial-mesenchymal transition (EMT) and cancer progression in colon tumors. To validate these findings experimentally, we analyzed the expression of SPIN.DOC in normal colorectal and three colorectal cancer cell lines by immunofluorescence and western blotting. SPIN.DOC was expressed at low level in human normal colon epithelial cells but was highly expressed in all the three colorectal cancer cell lines tested. Consistent with the scRNA data, overexpression of SPIN.DOC in CRC cell line, enhanced cell proliferation, cell migration, invasion, colony-forming capability and Wnt signaling. Moreover, these cells upregulate EMT-associated genes and cancer stem cell markers, as determined by qRT-PCR and immunoblotting. Enhanced spheroid formation in SPIN.DOC overexpressing cells further indicated increased stemness of the cells. Conversely, the knockdown of SPIN.DOC diminished the aforesaid phenotypes. Overall, these data suggest that SPIN.DOC promotes cancer cell proliferation, metastasis and regulates pluripotency and self-renewal of colorectal CSCs. Overall, our study highlights SPIN.DOC as a key essence of CRC by regulating cancer stemness and provides an opportunity of using SPIN.DOC, as a diagnostic and prognostic biomarker of colorectal cancer.
(© 2026. The Author(s) under exclusive licence to Japan Human Cell Society.)
Competing Interests: Declarations. Conflict of interest: The authors declare that they have no competing interests. AI declaration: The authors used ChatGPT-4 to improve the readability and language of this manuscript. The content was reviewed and edited by the authors, who take full responsibility for the final version.
References: Int J Oncol. 2014 Mar;44(3):693-9. (PMID: 24365974)
Front Immunol. 2019 Dec 16;10:2872. (PMID: 31921137)
Transl Cancer Res. 2025 Oct 31;14(10):6497-6519. (PMID: 41234894)
BMC Bioinformatics. 2020 May 15;21(1):191. (PMID: 32414321)
Oncol Rep. 2016 Jan;35(1):261-6. (PMID: 26498709)
Clin Cancer Res. 2021 Sep 1;27(17):4680-4684. (PMID: 33846198)
Int J Cancer. 2019 Jun 15;144(12):2992-3000. (PMID: 30536395)
Biochem Biophys Res Commun. 2019 Apr 9;511(3):498-503. (PMID: 30803761)
J Drug Target. 2024 Dec;32(1):33-44. (PMID: 38095181)
Curr Cancer Drug Targets. 2018;18(3):266-277. (PMID: 28669338)
Cancer Lett. 2024 Mar 1;584:216664. (PMID: 38253219)
Cells. 2023 Dec 28;13(1):. (PMID: 38201271)
Genome Biol. 2014;15(12):550. (PMID: 25516281)
J Hematol Oncol. 2017 May 5;10(1):101. (PMID: 28476164)
J Biol Chem. 2017 Dec 22;292(51):20808-20817. (PMID: 29061846)
CA Cancer J Clin. 2020 Jan;70(1):7-30. (PMID: 31912902)
Nat Methods. 2019 Dec;16(12):1289-1296. (PMID: 31740819)
J Biol Chem. 2020 Dec 4;295(49):16877-16887. (PMID: 32994220)
Stem Cells Int. 2021 Jan 6;2021:6667735. (PMID: 33505471)
Mol Biol Res Commun. 2025;14(4):283-289. (PMID: 40949799)
Int J Mol Med. 2022 Jan;49(1):. (PMID: 34713301)
Int J Cancer. 2017 Jun 15;140(12):2709-2715. (PMID: 28281292)
J Natl Compr Canc Netw. 2021 Mar 02;19(3):329-359. (PMID: 33724754)
Cancer Manag Res. 2023 May 23;15:435-448. (PMID: 37250384)
Mol Ther Oncolytics. 2021 May 08;21:183-206. (PMID: 34027052)
J Clin Invest. 2011 Mar;121(3):1064-74. (PMID: 21393860)
Biomolecules. 2023 Dec 14;13(12):. (PMID: 38136663)
Mol Cancer Res. 2024 Apr 2;22(4):337-346. (PMID: 38156967)
Signal Transduct Target Ther. 2022 Feb 28;7(1):60. (PMID: 35221332)
Nat Biotechnol. 2024 Feb;42(2):293-304. (PMID: 37231261)
Cancer Cell. 2023 Jun 12;41(6):1152-1169.e7. (PMID: 37172580)
Cancer Gene Ther. 2024 Aug;31(8):1201-1220. (PMID: 38877164)
Nat Cell Biol. 2014 Jun;16(6):488-94. (PMID: 24875735)
Cell Syst. 2019 Apr 24;8(4):329-337.e4. (PMID: 30954475)
Am J Cancer Res. 2020 Mar 01;10(3):727-742. (PMID: 32266087)
J Biol Res (Thessalon). 2021 Nov 23;28(1):22. (PMID: 34814951)
Epigenetics. 2011 Jan;6(1):9-15. (PMID: 20855937)
J Clin Med. 2023 Dec 21;13(1):. (PMID: 38202047)
Front Immunol. 2023 Apr 28;14:1148061. (PMID: 37187731)
Nat Commun. 2022 Jan 11;13(1):192. (PMID: 35017482)
MedGenMed. 2004 Aug 13;6(3):13. (PMID: 15520636)
Nat Commun. 2021 Feb 11;12(1):949. (PMID: 33574238)
Grant Information: Internal funding suppport Department of Molecular Biology and Biotechnology, Tezpur University
Contributed Indexing: Keywords: Cancer stem cell; Colorectal cancer; Oncogene; SPIN.DOC; Wnt signalling
Substance Nomenclature: 0 (beta Catenin)
Entry Date(s): Date Created: 20260819 Date Completed: 20260819 Latest Revision: 20261008
Update Code: 20261008
DOI: 10.1007/s13577-026-01442-9
PMID: 42616187
Database: MEDLINE
FullText Text:
  Availability: 0
CustomLinks:
  – Url: https://dx.doi.org/doi:10.1007/s13577-026-01442-9
    Name: EDS - Springer Nature Journals (s7799221)
    Category: fullText
    Text: View record at Springer
  – Url: https://resolver.ebsco.com/c/fiv2js/result?sid=EBSCO:cmedm&genre=article&issn=17490774&ISBN=&volume=39&issue=9&date=20260819&spage=&pages=&title=Human cell&atitle=Single-cell%20transcriptomics%20and%20functional%20analyses%20identified%20SPIN.DOC%20as%20a%20regulator%20for%20Wnt%20signaling%20and%20cancer%20stemness%20in%20colorectal%20cancer.&aulast=Devi%20KM&id=DOI:10.1007/s13577-026-01442-9
    Name: Full Text Finder (for New FTF UI) (ns324271)
    Category: fullText
    Text: Full Text Finder
    MouseOverText: Full Text Finder
Header DbId: cmedm
DbLabel: MEDLINE
An: 42616187
AccessLevel: 3
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Single-cell transcriptomics and functional analyses identified SPIN.DOC as a regulator for Wnt signaling and cancer stemness in colorectal cancer.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AU" term="%22Devi+KM%22">Devi KM</searchLink>; Department of Biotechnology, Manipur University, Imphal, 795003, India.<br /><searchLink fieldCode="AU" term="%22Abbas+E%22">Abbas E</searchLink>; Department of Molecular Biology and Biotechnology, Tezpur University, Napaam, Tezpur, 784028, Assam, India.<br /><searchLink fieldCode="AU" term="%22Singh+TD%22">Singh TD</searchLink>; Department of Biotechnology, Manipur University, Imphal, 795003, India.<br /><searchLink fieldCode="AU" term="%22Deka+R%22">Deka R</searchLink>; Department of Molecular Biology and Biotechnology, Tezpur University, Napaam, Tezpur, 784028, Assam, India.<br /><searchLink fieldCode="AU" term="%22Kumar+A%22">Kumar A</searchLink>; Department of Molecular Biology and Biotechnology, Tezpur University, Napaam, Tezpur, 784028, Assam, India.<br /><searchLink fieldCode="AU" term="%22Singh+LS%22">Singh LS</searchLink>; Department of Biotechnology, Manipur University, Imphal, 795003, India.<br /><searchLink fieldCode="AU" term="%22Singh+TR%22">Singh TR</searchLink>; Department of Molecular Biology and Biotechnology, Tezpur University, Napaam, Tezpur, 784028, Assam, India. rthiyam@tezu.ernet.in.
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%228912329%22">Human cell</searchLink> [Hum Cell] 2026 Aug 19; Vol. 39 (9). <i>Date of Electronic Publication: </i>2026 Aug 19.
– Name: TypePub
  Label: Publication Type
  Group: TypPub
  Data: Journal Article
– Name: Language
  Label: Language
  Group: Lang
  Data: English
– Name: TitleSource
  Label: Journal Info
  Group: Src
  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22Springer%22">Springer </searchLink><i>Country of Publication: </i>Japan <i>NLM ID: </i>8912329 <i>Publication Model: </i>Electronic <i>Cited Medium: </i>Internet <i>ISSN: </i>1749-0774 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2209147470%22">09147470 </searchLink><i>NLM ISO Abbreviation: </i>Hum Cell <i>Subsets: </i>MEDLINE
– Name: PublisherInfo
  Label: Imprint Name(s)
  Group: PubInfo
  Data: <i>Publication</i>: 2011- : Tokyo : Springer<br /><i>Original Publication</i>: Tōkyō-to : Hito Saibō Kenkyūkai : Kanishobo, Shōwa 63-nen [1988]-
– Name: SubjectMESH
  Label: MeSH Terms
  Group: Su
  Data: <searchLink fieldCode="MM" term="%22Wnt+Signaling+Pathway%22">Wnt Signaling Pathway*</searchLink>/<searchLink fieldCode="MM" term="%22Wnt+Signaling+Pathway+genetics%22">genetics</searchLink> <br /><searchLink fieldCode="MM" term="%22Wnt+Signaling+Pathway%22">Wnt Signaling Pathway*</searchLink>/<searchLink fieldCode="MM" term="%22Wnt+Signaling+Pathway+physiology%22">physiology</searchLink> <br /><searchLink fieldCode="MM" term="%22Colorectal+Neoplasms%22">Colorectal Neoplasms*</searchLink>/<searchLink fieldCode="MM" term="%22Colorectal+Neoplasms+genetics%22">genetics</searchLink> <br /><searchLink fieldCode="MM" term="%22Colorectal+Neoplasms%22">Colorectal Neoplasms*</searchLink>/<searchLink fieldCode="MM" term="%22Colorectal+Neoplasms+pathology%22">pathology</searchLink> <br /><searchLink fieldCode="MM" term="%22Neoplastic+Stem+Cells%22">Neoplastic Stem Cells*</searchLink>/<searchLink fieldCode="MM" term="%22Neoplastic+Stem+Cells+pathology%22">pathology</searchLink> <br /><searchLink fieldCode="MM" term="%22Single-Cell+Analysis%22">Single-Cell Analysis*</searchLink>/<searchLink fieldCode="MM" term="%22Single-Cell+Analysis+methods%22">methods</searchLink> <br /><searchLink fieldCode="MM" term="%22Gene+Expression%22">Gene Expression*</searchLink>/<searchLink fieldCode="MM" term="%22Gene+Expression+genetics%22">genetics</searchLink> <br /><searchLink fieldCode="MM" term="%22Carcinogenesis%22">Carcinogenesis*</searchLink>/<searchLink fieldCode="MM" term="%22Carcinogenesis+genetics%22">genetics</searchLink> <br /><searchLink fieldCode="MM" term="%22Transcriptome%22">Transcriptome*</searchLink>/<searchLink fieldCode="MM" term="%22Transcriptome+genetics%22">genetics</searchLink><br /><searchLink fieldCode="MH" term="%22Epithelial-Mesenchymal+Transition%22">Epithelial-Mesenchymal Transition</searchLink>/<searchLink fieldCode="MH" term="%22Epithelial-Mesenchymal+Transition+genetics%22">genetics</searchLink> ; <searchLink fieldCode="MH" term="%22Cell+Proliferation%22">Cell Proliferation</searchLink>/<searchLink fieldCode="MH" term="%22Cell+Proliferation+genetics%22">genetics</searchLink> ; <searchLink fieldCode="MH" term="%22beta+Catenin%22">beta Catenin</searchLink>/<searchLink fieldCode="MH" term="%22beta+Catenin+metabolism%22">metabolism</searchLink> ; <searchLink fieldCode="MH" term="%22beta+Catenin%22">beta Catenin</searchLink>/<searchLink fieldCode="MH" term="%22beta+Catenin+genetics%22">genetics</searchLink> ; <searchLink fieldCode="MH" term="%22Gene+Expression+Regulation%2C+Neoplastic%22">Gene Expression Regulation, Neoplastic</searchLink>/<searchLink fieldCode="MH" term="%22Gene+Expression+Regulation%2C+Neoplastic+genetics%22">genetics</searchLink> ; <searchLink fieldCode="MH" term="%22Humans%22">Humans</searchLink> ; <searchLink fieldCode="MH" term="%22Single-Cell+Gene+Expression+Analysis%22">Single-Cell Gene Expression Analysis</searchLink> ; <searchLink fieldCode="MH" term="%22Cell+Line%2C+Tumor%22">Cell Line, Tumor</searchLink> ; <searchLink fieldCode="MH" term="%22Disease+Progression%22">Disease Progression</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: The role of SPIN.DOC in tumorigenesis remains unclear. In this study, we utilized single-cell RNA sequencing (scRNA-seq) data to investigate SPIN.DOC expression in a cohort of normal, primary human colorectal cancer (CRC) and metastatic tissues from CRC patients. Our findings revealed differential expressions of SPIN.DOC across multiple cell clusters in different conditions and with the highest expression observed in tumor samples, suggesting its potential role in CRC progression. Further analysis showed that SPIN.DOC positive cell-cluster showed upregulation of β-catenin and cancer stem cell (CSC) marker genes, indicating its role in activating Wnt signaling for cell proliferation. Additionally, these cells also showed downregulation of genes associated with cell-cell junctions, promoting epithelial-mesenchymal transition (EMT) and cancer progression in colon tumors. To validate these findings experimentally, we analyzed the expression of SPIN.DOC in normal colorectal and three colorectal cancer cell lines by immunofluorescence and western blotting. SPIN.DOC was expressed at low level in human normal colon epithelial cells but was highly expressed in all the three colorectal cancer cell lines tested. Consistent with the scRNA data, overexpression of SPIN.DOC in CRC cell line, enhanced cell proliferation, cell migration, invasion, colony-forming capability and Wnt signaling. Moreover, these cells upregulate EMT-associated genes and cancer stem cell markers, as determined by qRT-PCR and immunoblotting. Enhanced spheroid formation in SPIN.DOC overexpressing cells further indicated increased stemness of the cells. Conversely, the knockdown of SPIN.DOC diminished the aforesaid phenotypes. Overall, these data suggest that SPIN.DOC promotes cancer cell proliferation, metastasis and regulates pluripotency and self-renewal of colorectal CSCs. Overall, our study highlights SPIN.DOC as a key essence of CRC by regulating cancer stemness and provides an opportunity of using SPIN.DOC, as a diagnostic and prognostic biomarker of colorectal cancer.<br /> (© 2026. The Author(s) under exclusive licence to Japan Human Cell Society.)
– Name: Abstract
  Label: Competing Interests
  Group: Ab
  Data: Declarations. Conflict of interest: The authors declare that they have no competing interests. AI declaration: The authors used ChatGPT-4 to improve the readability and language of this manuscript. The content was reviewed and edited by the authors, who take full responsibility for the final version.
– Name: Ref
  Label: References
  Group: RefInfo
  Data: Int J Oncol. 2014 Mar;44(3):693-9. (PMID: <searchLink fieldCode="PM" term="%2224365974%22">24365974)</searchLink><br />Front Immunol. 2019 Dec 16;10:2872. (PMID: <searchLink fieldCode="PM" term="%2231921137%22">31921137)</searchLink><br />Transl Cancer Res. 2025 Oct 31;14(10):6497-6519. (PMID: <searchLink fieldCode="PM" term="%2241234894%22">41234894)</searchLink><br />BMC Bioinformatics. 2020 May 15;21(1):191. (PMID: <searchLink fieldCode="PM" term="%2232414321%22">32414321)</searchLink><br />Oncol Rep. 2016 Jan;35(1):261-6. (PMID: <searchLink fieldCode="PM" term="%2226498709%22">26498709)</searchLink><br />Clin Cancer Res. 2021 Sep 1;27(17):4680-4684. (PMID: <searchLink fieldCode="PM" term="%2233846198%22">33846198)</searchLink><br />Int J Cancer. 2019 Jun 15;144(12):2992-3000. (PMID: <searchLink fieldCode="PM" term="%2230536395%22">30536395)</searchLink><br />Biochem Biophys Res Commun. 2019 Apr 9;511(3):498-503. (PMID: <searchLink fieldCode="PM" term="%2230803761%22">30803761)</searchLink><br />J Drug Target. 2024 Dec;32(1):33-44. (PMID: <searchLink fieldCode="PM" term="%2238095181%22">38095181)</searchLink><br />Curr Cancer Drug Targets. 2018;18(3):266-277. (PMID: <searchLink fieldCode="PM" term="%2228669338%22">28669338)</searchLink><br />Cancer Lett. 2024 Mar 1;584:216664. (PMID: <searchLink fieldCode="PM" term="%2238253219%22">38253219)</searchLink><br />Cells. 2023 Dec 28;13(1):. (PMID: <searchLink fieldCode="PM" term="%2238201271%22">38201271)</searchLink><br />Genome Biol. 2014;15(12):550. (PMID: <searchLink fieldCode="PM" term="%2225516281%22">25516281)</searchLink><br />J Hematol Oncol. 2017 May 5;10(1):101. (PMID: <searchLink fieldCode="PM" term="%2228476164%22">28476164)</searchLink><br />J Biol Chem. 2017 Dec 22;292(51):20808-20817. (PMID: <searchLink fieldCode="PM" term="%2229061846%22">29061846)</searchLink><br />CA Cancer J Clin. 2020 Jan;70(1):7-30. (PMID: <searchLink fieldCode="PM" term="%2231912902%22">31912902)</searchLink><br />Nat Methods. 2019 Dec;16(12):1289-1296. (PMID: <searchLink fieldCode="PM" term="%2231740819%22">31740819)</searchLink><br />J Biol Chem. 2020 Dec 4;295(49):16877-16887. (PMID: <searchLink fieldCode="PM" term="%2232994220%22">32994220)</searchLink><br />Stem Cells Int. 2021 Jan 6;2021:6667735. (PMID: <searchLink fieldCode="PM" term="%2233505471%22">33505471)</searchLink><br />Mol Biol Res Commun. 2025;14(4):283-289. (PMID: <searchLink fieldCode="PM" term="%2240949799%22">40949799)</searchLink><br />Int J Mol Med. 2022 Jan;49(1):. (PMID: <searchLink fieldCode="PM" term="%2234713301%22">34713301)</searchLink><br />Int J Cancer. 2017 Jun 15;140(12):2709-2715. (PMID: <searchLink fieldCode="PM" term="%2228281292%22">28281292)</searchLink><br />J Natl Compr Canc Netw. 2021 Mar 02;19(3):329-359. (PMID: <searchLink fieldCode="PM" term="%2233724754%22">33724754)</searchLink><br />Cancer Manag Res. 2023 May 23;15:435-448. (PMID: <searchLink fieldCode="PM" term="%2237250384%22">37250384)</searchLink><br />Mol Ther Oncolytics. 2021 May 08;21:183-206. (PMID: <searchLink fieldCode="PM" term="%2234027052%22">34027052)</searchLink><br />J Clin Invest. 2011 Mar;121(3):1064-74. (PMID: <searchLink fieldCode="PM" term="%2221393860%22">21393860)</searchLink><br />Biomolecules. 2023 Dec 14;13(12):. (PMID: <searchLink fieldCode="PM" term="%2238136663%22">38136663)</searchLink><br />Mol Cancer Res. 2024 Apr 2;22(4):337-346. (PMID: <searchLink fieldCode="PM" term="%2238156967%22">38156967)</searchLink><br />Signal Transduct Target Ther. 2022 Feb 28;7(1):60. (PMID: <searchLink fieldCode="PM" term="%2235221332%22">35221332)</searchLink><br />Nat Biotechnol. 2024 Feb;42(2):293-304. (PMID: <searchLink fieldCode="PM" term="%2237231261%22">37231261)</searchLink><br />Cancer Cell. 2023 Jun 12;41(6):1152-1169.e7. (PMID: <searchLink fieldCode="PM" term="%2237172580%22">37172580)</searchLink><br />Cancer Gene Ther. 2024 Aug;31(8):1201-1220. (PMID: <searchLink fieldCode="PM" term="%2238877164%22">38877164)</searchLink><br />Nat Cell Biol. 2014 Jun;16(6):488-94. (PMID: <searchLink fieldCode="PM" term="%2224875735%22">24875735)</searchLink><br />Cell Syst. 2019 Apr 24;8(4):329-337.e4. (PMID: <searchLink fieldCode="PM" term="%2230954475%22">30954475)</searchLink><br />Am J Cancer Res. 2020 Mar 01;10(3):727-742. (PMID: <searchLink fieldCode="PM" term="%2232266087%22">32266087)</searchLink><br />J Biol Res (Thessalon). 2021 Nov 23;28(1):22. (PMID: <searchLink fieldCode="PM" term="%2234814951%22">34814951)</searchLink><br />Epigenetics. 2011 Jan;6(1):9-15. (PMID: <searchLink fieldCode="PM" term="%2220855937%22">20855937)</searchLink><br />J Clin Med. 2023 Dec 21;13(1):. (PMID: <searchLink fieldCode="PM" term="%2238202047%22">38202047)</searchLink><br />Front Immunol. 2023 Apr 28;14:1148061. (PMID: <searchLink fieldCode="PM" term="%2237187731%22">37187731)</searchLink><br />Nat Commun. 2022 Jan 11;13(1):192. (PMID: <searchLink fieldCode="PM" term="%2235017482%22">35017482)</searchLink><br />MedGenMed. 2004 Aug 13;6(3):13. (PMID: <searchLink fieldCode="PM" term="%2215520636%22">15520636)</searchLink><br />Nat Commun. 2021 Feb 11;12(1):949. (PMID: <searchLink fieldCode="PM" term="%2233574238%22">33574238)</searchLink>
– Name: GrantInfo
  Label: Grant Information
  Group: Grant
  Data: Internal funding suppport Department of Molecular Biology and Biotechnology, Tezpur University
– Name: SubjectMinor
  Label: Contributed Indexing
  Group:
  Data: <i>Keywords: </i>Cancer stem cell; Colorectal cancer; Oncogene; SPIN.DOC; Wnt signalling
– Name: NumberCAS
  Label: Substance Nomenclature
  Group: ID
  Data: 0 (beta Catenin)
– Name: DateEntry
  Label: Entry Date(s)
  Group: Date
  Data: <i>Date Created: </i>20260819 <i>Date Completed: </i>20260819 <i>Latest Revision: </i>20261008
– Name: DateUpdate
  Label: Update Code
  Group: Date
  Data: 20261008
– Name: DOI
  Label: DOI
  Group: ID
  Data: 10.1007/s13577-026-01442-9
– Name: AN
  Label: PMID
  Group: ID
  Data: 42616187
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=cmedm&AN=42616187
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1007/s13577-026-01442-9
    Languages:
      – Code: eng
        Text: English
    Subjects:
      – SubjectFull: Epithelial-Mesenchymal Transition genetics
        Type: general
      – SubjectFull: Cell Proliferation genetics
        Type: general
      – SubjectFull: beta Catenin metabolism
        Type: general
      – SubjectFull: beta Catenin genetics
        Type: general
      – SubjectFull: Gene Expression Regulation, Neoplastic genetics
        Type: general
      – SubjectFull: Humans
        Type: general
      – SubjectFull: Single-Cell Gene Expression Analysis
        Type: general
      – SubjectFull: Cell Line, Tumor
        Type: general
      – SubjectFull: Disease Progression
        Type: general
      – SubjectFull: Wnt Signaling Pathway genetics
        Type: general
      – SubjectFull: Wnt Signaling Pathway physiology
        Type: general
      – SubjectFull: Colorectal Neoplasms genetics
        Type: general
      – SubjectFull: Colorectal Neoplasms pathology
        Type: general
      – SubjectFull: Neoplastic Stem Cells pathology
        Type: general
      – SubjectFull: Single-Cell Analysis methods
        Type: general
      – SubjectFull: Gene Expression genetics
        Type: general
      – SubjectFull: Carcinogenesis genetics
        Type: general
      – SubjectFull: Transcriptome genetics
        Type: general
    Titles:
      – TitleFull: Single-cell transcriptomics and functional analyses identified SPIN.DOC as a regulator for Wnt signaling and cancer stemness in colorectal cancer.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Devi KM
      – PersonEntity:
          Name:
            NameFull: Abbas E
      – PersonEntity:
          Name:
            NameFull: Singh TD
      – PersonEntity:
          Name:
            NameFull: Deka R
      – PersonEntity:
          Name:
            NameFull: Kumar A
      – PersonEntity:
          Name:
            NameFull: Singh LS
      – PersonEntity:
          Name:
            NameFull: Singh TR
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 19
              M: 08
              Text: 2026 Aug 19
              Type: published
              Y: 2026
          Identifiers:
            – Type: issn-electronic
              Value: 1749-0774
          Numbering:
            – Type: volume
              Value: 39
            – Type: issue
              Value: 9
          Titles:
            – TitleFull: Human cell
              Type: main
ResultId 1