Academic Journal
Single-cell transcriptomic analysis of ferroptosis-associated cell populations in the progression of diabetic retinopathy.
| Τίτλος: | Single-cell transcriptomic analysis of ferroptosis-associated cell populations in the progression of diabetic retinopathy. |
|---|---|
| Συγγραφείς: | Duan BF; The Affiliated Eye Hospital, Jiangxi Medical College, Nanchang University, 463 Bayi Road, Nanchang, 330006, Jiangxi, China., Shao YN; The Affiliated Eye Hospital, Jiangxi Medical College, Nanchang University, 463 Bayi Road, Nanchang, 330006, Jiangxi, China., Mao ZQ; The Affiliated Eye Hospital, Jiangxi Medical College, Nanchang University, 463 Bayi Road, Nanchang, 330006, Jiangxi, China., Li XN; The Affiliated Eye Hospital, Jiangxi Medical College, Nanchang University, 463 Bayi Road, Nanchang, 330006, Jiangxi, China., Fan HM; The Affiliated Eye Hospital, Jiangxi Medical College, Nanchang University, 463 Bayi Road, Nanchang, 330006, Jiangxi, China., Cai Y; The Affiliated Eye Hospital, Jiangxi Medical College, Nanchang University, 463 Bayi Road, Nanchang, 330006, Jiangxi, China., Zheng HW; The Affiliated Eye Hospital, Jiangxi Medical College, Nanchang University, 463 Bayi Road, Nanchang, 330006, Jiangxi, China., Peng SJ; The Affiliated Eye Hospital, Jiangxi Medical College, Nanchang University, 463 Bayi Road, Nanchang, 330006, Jiangxi, China., You ZP; The Affiliated Eye Hospital, Jiangxi Medical College, Nanchang University, 463 Bayi Road, Nanchang, 330006, Jiangxi, China. yzp7477@163.com. |
| Πηγή: | International ophthalmology [Int Ophthalmol] 2026 Aug 07; Vol. 46 (1). Date of Electronic Publication: 2026 Aug 07. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Kluwer Country of Publication: Netherlands NLM ID: 7904294 Publication Model: Electronic Cited Medium: Internet ISSN: 1573-2630 (Electronic) Linking ISSN: 01655701 NLM ISO Abbreviation: Int Ophthalmol Subsets: MEDLINE |
| Imprint Name(s): | Publication: Dordrecht : Kluwer Original Publication: The Hague, Junk. |
| Ιατρικοί όροι (MeSH): | Diabetic Retinopathy*/genetics , Diabetic Retinopathy*/metabolism , Diabetic Retinopathy*/pathology , Ferroptosis*/genetics , Gene Expression Profiling*/methods , Transcriptome* , Gene Expression Regulation*, Ependymoglial Cells/metabolism ; Ependymoglial Cells/pathology ; Animals ; Rats ; Disease Progression ; Single-Cell Gene Expression Analysis ; Single-Cell Analysis ; Diabetes Mellitus, Experimental ; Disease Models, Animal ; Rats, Sprague-Dawley ; Male |
| Περίληψη: | Background: Diabetic retinopathy (DR), a common microvascular complication of diabetes mellitus, has been associated with ferroptosis-related pathological mechanisms. Evidence indicates that ferroptosis may contribute to retinal injury and disease progression. The present analysis aimed to characterize ferroptosis-associated cell subsets and their roles in the progression of DR. Method: Single-cell RNA sequencing data derived from diabetic rat models of DR were obtained from the Gene Expression Omnibus database. A total of 464 ferroptosis-related genes were used to calculate ferroptosis scores. Data processing and analysis were performed using the Seurat R package, with differentially expressed genes identified via the FindAllMarkers function. Functional enrichment analyses were conducted using the clusterProfiler package. Protein-protein interaction networks were constructed using the STRING database, and intercellular communication was predicted using CellChat. Key findings were validated through reverse transcription quantitative polymerase chain reaction (RT-qPCR). Results: Cell clustering identified nine principal retinal cell types. Compared with normal control groups, the DR group demonstrated altered proportions of Müller cells, vascular endothelial cells, microglia, and cone photoreceptor cells. Ferroptosis-score-related differences were most evident in Müller cells and rod cells. In the revised WT-baseline sensitivity analysis, the high-ratio ferroptosis-score cell distribution remained significantly altered in Rod and Müller cells after multiple-testing correction. Conclusion: This study identified key ferroptosis-associated retinal cell populations, with an emphasis on the functional roles of ferroptosis-associated Müller cell subsets in the context of DR. These findings provide further insight into the cellular mechanisms underlying DR and highlight potential molecular targets for therapeutic intervention. (© 2026. The Author(s), under exclusive licence to Springer Nature B.V.) |
| Competing Interests: | Declarations. Ethics approval statement: This study received approval from the ethics committees of both the Affiliated Eye Hospital of Nanchang University (No. YLP20250123). Conflict of interest: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. |
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| Grant Information: | 82260212 the National Natural Science Foundation of China |
| Contributed Indexing: | Keywords: Cell communication; Diabetic retinopathy; Differentially expressed genes; Ferroptosis; Single-cell analysis |
| Entry Date(s): | Date Created: 20260807 Date Completed: 20260808 Latest Revision: 20261008 |
| Update Code: | 20261008 |
| DOI: | 10.1007/s10792-026-04197-2 |
| PMID: | 42568025 |
| Βάση Δεδομένων: | MEDLINE |
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