Academic Journal
[Mechanism research of baicalein in improving alveolar epithelial cell senescence via downregulating TGF-β1/Smad3 signal].
| Τίτλος: | [Mechanism research of baicalein in improving alveolar epithelial cell senescence via downregulating TGF-β1/Smad3 signal]. |
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| Συγγραφείς: | Gu JX; College of Pharmaceutical Sciences,Suzhou Medical College of Soochow University Suzhou 215123,China., Zhang JW; College of Pharmaceutical Sciences,Suzhou Medical College of Soochow University Suzhou 215123,China., Jiang XG; College of Pharmaceutical Sciences,Suzhou Medical College of Soochow University Suzhou 215123,China. |
| Πηγή: | Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica [Zhongguo Zhong Yao Za Zhi] 2026 Jun; Vol. 51 (11), pp. 3060-3066. |
| Τύπος έκδοσης: | English Abstract; Journal Article |
| Γλώσσα: | Chinese |
| Στοιχεία περιοδικού: | Publisher: Zhongguo yao xue hui : Zhongguo Zhong yi yan jiu yuan Zhong yao yan jiu suo Country of Publication: China NLM ID: 8913656 Publication Model: Print Cited Medium: Print ISSN: 1001-5302 (Print) Linking ISSN: 10015302 NLM ISO Abbreviation: Zhongguo Zhong Yao Za Zhi Subsets: MEDLINE |
| Imprint Name(s): | Publication: Beijing : Zhongguo yao xue hui : Zhongguo Zhong yi yan jiu yuan Zhong yao yan jiu suo Original Publication: [Beijing] : Zhongguo yao xue hui : [Zhongguo Zhong yi yan jiu yuan Zhong yao yan jiu suo, 1989- |
| Ιατρικοί όροι (MeSH): | Flavanones*/pharmacology , Smad3 Protein*/metabolism , Smad3 Protein*/genetics , Transforming Growth Factor beta1*/metabolism , Transforming Growth Factor beta1*/genetics , Cellular Senescence*/drug effects , Alveolar Epithelial Cells*/drug effects , Alveolar Epithelial Cells*/metabolism , Alveolar Epithelial Cells*/cytology, Signal Transduction/drug effects ; Down-Regulation/drug effects ; Plasminogen Activator Inhibitor 1/metabolism ; Plasminogen Activator Inhibitor 1/genetics ; Epithelial Cells/drug effects ; Epithelial Cells/metabolism ; Smad2 Protein/genetics ; Smad2 Protein/metabolism ; Animals ; Humans ; Cell Line ; Rats |
| Περίληψη: | This study aims to investigate the effect and mechanism of baicalein in improving alveolar epithelial cell senescence. A cellular model of alveolar epithelial cell senescence induced by transforming growth factor-β1(TGF-β1) was established. Cells were randomly divided into control group, model group, baicalein group, small interfering RNA(siRNA) targeting Smad family member 2(Smad2) group, siRNA targeting Smad3 group, and baicalein + Smad3 plasmid group. Western blot was employed to detect the protein expression level of plasminogen activator inhibitor-1(PAI-1), a marker protein of alveolar epithelial cell senescence. Senescence-associated β-galactosidase(SA-β-gal) staining was used to measure the percentage of SA-β-gal-positive cells. The results show that baicalein can significantly inhibit the protein expression of PAI-1 in TGF-β1-induced alveolar epithelial cells and markedly reduce the percentage of SA-β-gal-positive cells in TGF-β1-induced alveolar epithelial cells. Baicalein can also significantly decrease the phosphorylation levels of Smad2 protein and Smad3 protein in TGF-β1-induced alveolar epithelial cells. Knockdown of Smad2 does not affect the protein expression level of PAI-1 in TGF-β1-induced alveolar epithelial cells, nor does it alter the percentage of SA-β-gal-positive cells in TGF-β1-induced alveolar epithelial cells. In contrast, knockdown of Smad3 can significantly downregulate the protein expression level of PAI-1 and reduce the percentage of SA-β-gal-positive cells in TGF-β1-induced alveolar epithelial cells. Overexpression of Smad3 can notably reverse the inhibitory effects of baicalein on PAI-1 protein expression and the percentage of SA-β-gal-positive cells in TGF-β1-induced alveolar epithelial cells. In conclusion, baicalein improves alveolar epithelial cell senescence by downregulating the TGF-β1/Smad3 signaling, but this effect might be independent of Smad2 signaling. |
| Contributed Indexing: | Keywords: Smad family member 3; alveolar epithelial cell senescence; baicalein; plasminogen activator inhibitor-1; pulmonary fibrosis; transforming growth factor-β1 |
| Substance Nomenclature: | 49QAH60606 (baicalein) 0 (Flavanones) 0 (Smad3 Protein) 0 (Transforming Growth Factor beta1) 0 (Plasminogen Activator Inhibitor 1) 0 (Smad2 Protein) |
| Entry Date(s): | Date Created: 20260802 Date Completed: 20260802 Latest Revision: 20260802 |
| Update Code: | 20260803 |
| DOI: | 10.19540/j.cnki.cjcmm.20260320.701 |
| PMID: | 42543267 |
| Βάση Δεδομένων: | MEDLINE |
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