Academic Journal
Design guide for synthetic small regulatory RNAs for high-efficiency gene knockdown in bacteria.
| Τίτλος: | Design guide for synthetic small regulatory RNAs for high-efficiency gene knockdown in bacteria. |
|---|---|
| Συγγραφείς: | Ren J; Department of Biomedical Engineering, Chung-Ang University, Seoul 06974, Republic of Korea., Kim Y; Department of Biomedical Engineering, Chung-Ang University, Seoul 06974, Republic of Korea., Nam HJ; Department of Biomedical Engineering, Chung-Ang University, Seoul 06974, Republic of Korea., Lee HM; Department of Biomedical Engineering, Chung-Ang University, Seoul 06974, Republic of Korea., Na D; Department of Biomedical Engineering, Chung-Ang University, Seoul 06974, Republic of Korea. |
| Πηγή: | Journal of microbiology (Seoul, Korea) [J Microbiol] 2026 Jul; Vol. 64 (7), pp. e2603026. Date of Electronic Publication: 2026 Jul 06. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Microbiological Society Of Korea Country of Publication: Korea (South) NLM ID: 9703165 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1976-3794 (Electronic) Linking ISSN: 12258873 NLM ISO Abbreviation: J Microbiol Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Seoul : Microbiological Society Of Korea |
| Ιατρικοί όροι (MeSH): | Gene Knockdown Techniques*/methods , Escherichia coli*/genetics , Escherichia coli*/metabolism , RNA, Small Untranslated*/genetics , RNA, Small Untranslated*/chemical synthesis , Bacteria*/genetics, Synthetic Biology/methods ; Metabolic Engineering/methods ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; RNA, Messenger/chemistry ; Host Factor 1 Protein/genetics ; Host Factor 1 Protein/metabolism ; RNA, Bacterial/genetics ; Escherichia coli Proteins/genetics ; Escherichia coli Proteins/metabolism ; Gene Expression Regulation, Bacterial |
| Περίληψη: | Small regulatory RNAs (sRNAs) are short noncoding RNAs that can fine-control the expression of target genes in trans at the post-transcriptional level in prokaryotes. Since there is a big challenge in constructing gene-knockout libraries, synthetic sRNAs have attracted considerable interest in synthetic biology and metabolic engineering, as they enable targeted gene knockdown without requiring chromosomal modifications. However, the development of high-efficiency synthetic sRNAs remains a demanding task that requires careful consideration of multiple design factors. Here, we provide a detailed protocol for the design and construction of synthetic sRNAs, detailing key design principles and critical optimization factors, including scaffold selection, target mRNA binding affinity, target mRNA secondary structure, and Hfq expression levels. This strategy can be broadly applied across E. coli and other bacterial hosts to modulate gene expression, thereby supporting versatile applications in synthetic biology and metabolic engineering. |
| Grant Information: | National Research Foundation of Korea; RS-2025-02303886 Ministry of Science and ICT; Chung-Ang University |
| Contributed Indexing: | Keywords: gene expression; knockdown; metabolic engineering; post-transcriptional regulation; synthetic biology; synthetic sRNA |
| Substance Nomenclature: | 0 (RNA, Small Untranslated) 0 (RNA, Messenger) 0 (Host Factor 1 Protein) 0 (RNA, Bacterial) 0 (Escherichia coli Proteins) |
| Entry Date(s): | Date Created: 20260802 Date Completed: 20260802 Latest Revision: 20260802 |
| Update Code: | 20260803 |
| DOI: | 10.71150/jm.2603026 |
| PMID: | 42543183 |
| Βάση Δεδομένων: | MEDLINE |
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