Academic Journal
Προβολή σε EDS

A preliminary investigation of antimicrobial, antibiofilm, and antiproliferative properties of a seed-derived endophytic fungus (Eremothecium coryli) from Basella alba L.: a crude extract-based screening study.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος: A preliminary investigation of antimicrobial, antibiofilm, and antiproliferative properties of a seed-derived endophytic fungus (Eremothecium coryli) from Basella alba L.: a crude extract-based screening study.
Συγγραφείς: Saha M; Department of Cell Biology and Molecular Genetics, Sri Devaraj Urs Academy of Higher Education and Research, Kolar, 563103, Karnataka, India., Pamidimukkala K; Department of Cell Biology and Molecular Genetics, Sri Devaraj Urs Academy of Higher Education and Research, Kolar, 563103, Karnataka, India. kiranmayee@sduaher.ac.in.
Πηγή: Antonie van Leeuwenhoek [Antonie Van Leeuwenhoek] 2026 Aug 01; Vol. 119 (9). Date of Electronic Publication: 2026 Aug 01.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Springer Country of Publication: Netherlands NLM ID: 0372625 Publication Model: Electronic Cited Medium: Internet ISSN: 1572-9699 (Electronic) Linking ISSN: 00036072 NLM ISO Abbreviation: Antonie Van Leeuwenhoek Subsets: MEDLINE
Imprint Name(s): Publication: 2004- : Berlin : Springer
Original Publication: Wageningen, Netherland [etc.] Veenman [etc.]
Ιατρικοί όροι (MeSH): Biofilms*/drug effects , Cell Proliferation*/drug effects , Endophytes*/chemistry , Endophytes*/isolation & purification , Ascomycota*/chemistry , Anti-Infective Agents*/pharmacology , Anti-Infective Agents*/chemistry , Anti-Infective Agents*/isolation & purification , Anti-Bacterial Agents*/pharmacology , Antineoplastic Agents*/pharmacology , Complex Mixtures*/pharmacology , Complex Mixtures*/chemistry, Seeds/microbiology ; Bacteria/drug effects ; Humans ; Cell Line, Tumor ; Microbial Sensitivity Tests
Περίληψη: This study presents a preliminary investigation into the antimicrobial, antibiofilm, and antiproliferative potential of Eremothecium coryli, an endophytic fungus isolated from the seeds of Basella alba L. The biological activities were evaluated using a crude extract-based screening study, employing the lyophilized cell-free supernatant (LCFS) of the fungal culture. Antibacterial and antibiofilm activities were assessed against selected multidrug-resistant bacterial pathogens, while antiproliferative activity was evaluated against breast cancer cell lines and a normal breast epithelial cell line. The LCFS demonstrated measurable antibacterial activity and significant biofilm-inhibition in a concentration-dependent manner. In cytotoxicity assays, selective growth inhibition of breast cancer cell lines was observed when compared with normal cells. Chemical profiling using Liquid Chromatography-Mass Spectrometry and Gas Chromatography-Mass Spectrometry enabled putative metabolite identification, revealing the presence of compounds previously reported to exhibit antimicrobial and anticancer activities. However, these identifications are tentative and based on spectral library matching. Overall, this preliminary investigation highlights the biological potential of E. coryli-derived metabolites. Further purification and validation are necessary to confirm compound identities, elucidate mechanisms of action, and assess in vivo relevance.
(© 2026. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)
Competing Interests: Declarations. Competing interests: The authors have no relevant financial or non-financial interests to disclose. Ethics declaration: Not applicable.
References: Ababutain IM, Aldosary SK, Aljuraifani AA et al (2021) Identification and antibacterial characterization of Endophytic Fungi from Artemisia sieberi. Int J Microbiol 2021:6651020. https://doi.org/10.1155/2021/6651020. (PMID: 10.1155/2021/6651020337470877960065)
Brayton CF (1986) Dimethyl sulfoxide (DMSO): a review. Cornell Vet 76(1):61–90. (PMID: 3510103)
Brito R, Silva G, Farias T, Ferreira P, Ferreira S (2017) Standardization of the safety level of the use of DMSO in viability assays in bacterial cells. MOL2NET, International Conference Series on Multidisciplinary Sciences http://sciforum.net/conference/mol2net-03 http:// https://doi.org/10.3390/MOL2NET-03-04980 .
Chatchawal C, Nualkaew N, Preeprame S, Porasuphatana S, Priprame A (2010) Physical and biological properties of mucilage from Basella alba L. stem and its gel formulation. Isan J Pharma Sci 6(3):104–112.
Clavel AJC, Villanueva JM, Yee JM (2019) Evaluation of the antimicrobial activity of ointments containing the ethanolic extract of Basella alba Linn leaves (Family basellaceae). [Bachelor's thesis, De La Salle Medical and Health Sciences Institute]. GreenPrints. https://greenprints.dlshsi.edu.ph/bsp/15/.
Do AD, Quang HP, Phan QK (2025) Probiotic cell-free supernatant as effective antimicrobials against Klebsiella pneumoniae and reduce antibiotic resistance development. Int Microbiol 28:623–632. https://doi.org/10.1007/s10123-024-00575-x. (PMID: 10.1007/s10123-024-00575-x39117894)
Ediriweera MK, Tennekoon KH, Samarakoon SR (2019) In vitro assays and techniques utilized in anticancer drug discovery. J Applied Toxicol 39(1):38–71. https://doi.org/10.1002/jat.3658. (PMID: 10.1002/jat.3658)
Hashem AH, Attia MS, Kandil EK, et al (2023) Bioactive compounds and biomedical applications of endophytic fungi: a recent review. Microb Cell Fact 6 22(1):107. https://doi.org/10.1186/s12934-023-02118-x . Erratum in: Microb Cell Fact. 22(1):122. 10.1186/s12934-023-02131-0.
Hema P, Murali M, Thriveni MC (2015) Phytochemical analysis and antibacterial activity of endophytic fungi isolated from Basella rubra L.,-A medicinal plant. J Pure Appl Microbiol 9(4):2971–2978.
Hridoy M, Gorapi MZH et al (2022) Putative anticancer compounds from plant derived endophytic fungi: a review. Molecules 27(1):296. https://doi.org/10.3390/molecules27010296. (PMID: 10.3390/molecules27010296350115278746379)
Ibrahim TA, Ajongbolo KF, Aladekoyi G (2014) Phytochemical screening and antimicrobial activity of crude extracts of Basella alba and Helianthus annuus on selected food pathogens. J Microbiol Biotechnol 3(2):27–31.
Islam M, Rahi M, Jahangir CA, Rahman MH, Jerin I et al (2018) In vivo anticancer activity of Basella alba leaf and seed extracts against Ehrlich’s ascites carcinoma (EAC) cell line. Evi Based Complement Alternat Med 2018:1537896. https://doi.org/10.1155/2018/1537896. (PMID: 10.1155/2018/1537896)
Kamiloglu S, Sari G, Ozdal T, Capanoglu E (2020) Guidelines for cell viability assays. Food Front 1(3):332–349. (PMID: 10.1002/fft2.44)
Katoch M, Phull S, Vaid S, Singh S (2017) Diversity, Phylogeny, anticancer and antimicrobial potential of fungal endophytes associated with Monarda citriodora L. BioMed Central Microbiol 17(1):1–3. https://doi.org/10.1186/s12866-017-0961-2. (PMID: 10.1186/s12866-017-0961-2)
Kaur N, Arora DS (2020) Prospecting the antimicrobial and antibiofilm potential of Chaetomium globosum an endophytic fungus from Moringa oleifera. AMB Express 10(1):1–3. https://doi.org/10.1186/s13568-020-01143-y. (PMID: 10.1186/s13568-020-01143-y)
Kilari BP, Kotakadi VS, Penchalaneni J (2016) Anti-proliferative and apoptotic effects of Basella rubra (L.) Against 1, 2-Dimethyl Hydrazine-induced colon carcinogenesis in rats. Asian Pacific J Cancer Prevent 17(1) 73–80. http:// https://doi.org/10.7314/APJCP.2016.17.1.73 .
Kong YL, Tan JS, Goh CF (2025) Plant-Based Protein Fermentation Medium Optimization for Antimicrobial Cell-Free Supernatant Production by Lactiplantibacillus plantarum K014 Against Cutibacterium acnes Probiotics and Antimicro. Prot. https://doi.org/10.1007/s12602-025-10742-9. (PMID: 10.1007/s12602-025-10742-9)
Morombaye SM, Kangogo M, Revathi G, Nyerere A, Ochora J (2018) Evaluation of the antimicrobial effect of Nepeta cataria and Basella alba against clinically resistant Acinetobacter baumannii in Nairobi Kenya. Adv Microbiol 8(10):790–803. https://doi.org/10.4236/aim.2018.810052. (PMID: 10.4236/aim.2018.810052)
Moutusi S, Buela Parivallal P, Prasannakumar MK, Kiranmayee P (2019) Morphological and molecular characterization of culturable leaf endophytic fungi from Malabar Spinach: The first report. Studies in Fungi 1:192–204. https://doi.org/10.5943/sif/4/1/21. (PMID: 10.5943/sif/4/1/21)
Niu J, Li M, Wang Y (2024) Cell proliferation and cytotoxicity assays, the fundamental drug discovery. Int J Drug Discov Pharmacol 3(3):100013. https://doi.org/10.5394/ijddp.2024.100013. (PMID: 10.5394/ijddp.2024.100013)
Ogofure AG, Pelo SP, Green E (2024) Identification and assessment of secondary metabolites from three fungal endophytes of Solanum mauritianum against public health pathogens. Molecules 29(20):4924. https://doi.org/10.3390/molecules29204924. (PMID: 10.3390/molecules292049243945929211510704)
Ogofure AG, Sebola TE, Green E (2025) Metabolic profile and bioactivity of fungal endophytes isolated from Crinum macowanii. BMC Comple Med Therapies 25(1):269. https://doi.org/10.1186/s12906-025-05011-9. (PMID: 10.1186/s12906-025-05011-9)
Paul AK, Al Arif H, Seraj S, Nahar A, Nasrin D, Chowdhury MH et al (2011) A survey of plant items eaten by the low income groups of the rural population of Talbunia village in Bagerhat district, Bangladesh with an account of their folk medicinal applications. Adv Natural and App Sci 5(1):132–145.
Qayoom H, Alkhanani M, Almilaibary A, Alsagaby SA, Mir MA (2023) A network pharmacology-based investigation of brugine reveals its multi-target molecular mechanism against Breast Cancer. Med Oncol 40(7):202. https://doi.org/10.1007/s12032-023-02067-w. (PMID: 10.1007/s12032-023-02067-w37308611)
Riffiani R, Wada T, Shimomura N, Yamaguchi T, Aimi T (2021) An optimized method for high-quality DNA extraction medicinal fungi Mycoleptodonoides aitchisonii for whole genome sequencing. IOP Conf. Ser.: Earth Environ. Sci. 948: 012032. https://doi.org/10.1088/1755-1315/948/1/012032.
Rosdee S, Wisessombat S, Tayeh M, Malakul R, Phanaksri T, Sianglum W (2025) Antibacterial activity of the endophytic fungal extracts and synergistic effects of combinations of ethylenediaminetetraacetic acid (EDTA) against Pseudomonas aeruginosa and Escherichia coli. PeerJ 13:e19074. https://doi.org/10.7717/peerj.19074. (PMID: 10.7717/peerj.190744006122511890036)
Saha M, Manasa AP, Prasannakumar MK, Pamidimukkala K (2024) Diversity pattern of cultivable endophytic microbes from various parts of two cultivars of indian spinach: Basella. Environ Eco Res 12(4):383–408. https://doi.org/10.13189/eer.2024.120405. (PMID: 10.13189/eer.2024.120405)
Saha M, Kiranmayee P (2024) First report on a wonder endophyte Coma circularis from Basella rubra L. stem: ahouse of pharmaceutically active compounds. The Microbe 4:100099. https://doi.org/10.1016/j.microb.2024.100099.
Sahoo S, Subban K, Chelliah J (2021) Diversity of marine macro-algicolous endophytic fungi and cytotoxic potential of Biscogniauxia petrensis metabolites against cancer cell lines. Front Microbiol 12:1472. https://doi.org/10.3389/fmicb.2021.650177. (PMID: 10.3389/fmicb.2021.650177)
Sharma D, Misba L, Khan AU (2019) Antibiotics versus biofilm: an emerging battleground in microbial communities. Antimicrob Resist Infect Control 8:76. https://doi.org/10.1186/s13756-019-0533-3. (PMID: 10.1186/s13756-019-0533-3311311076524306)
Singh A, Singh DK, Kharwar RN, White JF, Gond SK (2021) Fungal endophytes as efficient sources of plant-derived bioactive compounds and their prospective applications in natural product drug discovery: insights, avenues and challenges. Microorganisms 9(1):197. https://doi.org/10.3390/microorganisms9010197. (PMID: 10.3390/microorganisms9010197334779107833388)
Sithranga Boopathy N, Kathiresan K (2010) Anticancer drugs from marine flora: an overview. J Oncol 2010:214186. https://doi.org/10.1155/2010/214186. (PMID: 10.1155/2010/21418621461373)
Sornsenee P, Chatatikun M, Mitsuwan W, Kongpol K, Kooltheat N (2021) Lyophilized cell-free supernatants of Lactobacillus isolates exhibited antibiofilm, antioxidant, and reduces nitric oxide activity in lipopolysaccharide-stimulated RAW 264.7 cells. PeerJ 9:e12586. https://doi.org/10.7717/peerj.12586. (PMID: 10.7717/peerj.12586349092858641486)
Techaoei S, Jirayuthcharoenkul C, Jarmkom K et al (2020) Chemical evaluation and antibacterial activity of novel bioactive compounds from endophytic fungi in Nelumbo nucifera. Saudi J Biol Sci 27(11):2883–2889. https://doi.org/10.1016/j.sjbs.2020.08.037. (PMID: 10.1016/j.sjbs.2020.08.037331008437569113)
Techaoei S, Jarmkom K, Dumrongphuttidecha T, Khobjai W (2021a) Evaluation of the stability and antibacterial activity of crude extracts of hydro-endophytic fungi. J Advan Pharma Technol Res 12(1):61. https://doi.org/10.4103/japtr.JAPTR_114_20. (PMID: 10.4103/japtr.JAPTR_114_20)
Techaoei S, Jarmkom K, Dumrongphuttidecha T, Khobjai W (2021b) Evaluation of the stability and antibacterial activity of crude extracts of hydro-endophytic fungi. J Adv Pharm Technol Res 12(1):61–66. https://doi.org/10.4103/japtr.JAPTR_114_20. (PMID: 10.4103/japtr.JAPTR_114_20335323577832182)
Wen J, Okyere SK, Wang S et al (2022) Endophytic fungi: an effective alternative source of plant-derived bioactive compounds for pharmacological studies. J Fungi (Basel) 8(2):205. https://doi.org/10.3390/jof8020205. (PMID: 10.3390/jof8020205352059598877053)
Contributed Indexing: Keywords: Biofilm; Breast cancer cell lines; Initial cell attachment; Mass spectrometry; Multidrug-resistant bacteria
Substance Nomenclature: 0 (Anti-Infective Agents)
0 (Anti-Bacterial Agents)
0 (Antineoplastic Agents)
0 (Complex Mixtures)
Entry Date(s): Date Created: 20260801 Date Completed: 20260801 Latest Revision: 20260905
Update Code: 20260905
DOI: 10.1007/s10482-026-02368-w
PMID: 42541521
Βάση Δεδομένων: MEDLINE
FullText Text:
  Availability: 0
CustomLinks:
  – Url: https://dx.doi.org/doi:10.1007/s10482-026-02368-w
    Name: EDS - Springer Nature Journals (s7799221)
    Category: fullText
    Text: View record at Springer
  – Url: https://resolver.ebsco.com/c/fiv2js/result?sid=EBSCO:cmedm&genre=article&issn=15729699&ISBN=&volume=119&issue=9&date=20260801&spage=&pages=&title=Antonie van Leeuwenhoek&atitle=A%20preliminary%20investigation%20of%20antimicrobial%2C%20antibiofilm%2C%20and%20antiproliferative%20properties%20of%20a%20seed-derived%20endophytic%20fungus%20%28Eremothecium%20coryli%29%20from%20Basella%20alba%20L.%3A%20a%20crude%20extract-based%20screening%20study.&aulast=Saha%20M&id=DOI:10.1007/s10482-026-02368-w
    Name: Full Text Finder (for New FTF UI) (ns324271)
    Category: fullText
    Text: Full Text Finder
    MouseOverText: Full Text Finder
Header DbId: cmedm
DbLabel: MEDLINE
An: 42541521
AccessLevel: 3
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: A preliminary investigation of antimicrobial, antibiofilm, and antiproliferative properties of a seed-derived endophytic fungus (Eremothecium coryli) from Basella alba L.: a crude extract-based screening study.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AU" term="%22Saha+M%22">Saha M</searchLink>; Department of Cell Biology and Molecular Genetics, Sri Devaraj Urs Academy of Higher Education and Research, Kolar, 563103, Karnataka, India.<br /><searchLink fieldCode="AU" term="%22Pamidimukkala+K%22">Pamidimukkala K</searchLink>; Department of Cell Biology and Molecular Genetics, Sri Devaraj Urs Academy of Higher Education and Research, Kolar, 563103, Karnataka, India. kiranmayee@sduaher.ac.in.
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%220372625%22">Antonie van Leeuwenhoek</searchLink> [Antonie Van Leeuwenhoek] 2026 Aug 01; Vol. 119 (9). <i>Date of Electronic Publication: </i>2026 Aug 01.
– Name: TypePub
  Label: Publication Type
  Group: TypPub
  Data: Journal Article
– Name: Language
  Label: Language
  Group: Lang
  Data: English
– Name: TitleSource
  Label: Journal Info
  Group: Src
  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22Springer%22">Springer </searchLink><i>Country of Publication: </i>Netherlands <i>NLM ID: </i>0372625 <i>Publication Model: </i>Electronic <i>Cited Medium: </i>Internet <i>ISSN: </i>1572-9699 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2200036072%22">00036072 </searchLink><i>NLM ISO Abbreviation: </i>Antonie Van Leeuwenhoek <i>Subsets: </i>MEDLINE
– Name: PublisherInfo
  Label: Imprint Name(s)
  Group: PubInfo
  Data: <i>Publication</i>: 2004- : Berlin : Springer<br /><i>Original Publication</i>: Wageningen, Netherland [etc.] Veenman [etc.]
– Name: SubjectMESH
  Label: MeSH Terms
  Group: Su
  Data: <searchLink fieldCode="MM" term="%22Biofilms%22">Biofilms*</searchLink>/<searchLink fieldCode="MM" term="%22Biofilms+drug+effects%22">drug effects</searchLink> <br /><searchLink fieldCode="MM" term="%22Cell+Proliferation%22">Cell Proliferation*</searchLink>/<searchLink fieldCode="MM" term="%22Cell+Proliferation+drug+effects%22">drug effects</searchLink> <br /><searchLink fieldCode="MM" term="%22Endophytes%22">Endophytes*</searchLink>/<searchLink fieldCode="MM" term="%22Endophytes+chemistry%22">chemistry</searchLink> <br /><searchLink fieldCode="MM" term="%22Endophytes%22">Endophytes*</searchLink>/<searchLink fieldCode="MM" term="%22Endophytes+isolation+%26+purification%22">isolation & purification</searchLink> <br /><searchLink fieldCode="MM" term="%22Ascomycota%22">Ascomycota*</searchLink>/<searchLink fieldCode="MM" term="%22Ascomycota+chemistry%22">chemistry</searchLink> <br /><searchLink fieldCode="MM" term="%22Anti-Infective+Agents%22">Anti-Infective Agents*</searchLink>/<searchLink fieldCode="MM" term="%22Anti-Infective+Agents+pharmacology%22">pharmacology</searchLink> <br /><searchLink fieldCode="MM" term="%22Anti-Infective+Agents%22">Anti-Infective Agents*</searchLink>/<searchLink fieldCode="MM" term="%22Anti-Infective+Agents+chemistry%22">chemistry</searchLink> <br /><searchLink fieldCode="MM" term="%22Anti-Infective+Agents%22">Anti-Infective Agents*</searchLink>/<searchLink fieldCode="MM" term="%22Anti-Infective+Agents+isolation+%26+purification%22">isolation & purification</searchLink> <br /><searchLink fieldCode="MM" term="%22Anti-Bacterial+Agents%22">Anti-Bacterial Agents*</searchLink>/<searchLink fieldCode="MM" term="%22Anti-Bacterial+Agents+pharmacology%22">pharmacology</searchLink> <br /><searchLink fieldCode="MM" term="%22Antineoplastic+Agents%22">Antineoplastic Agents*</searchLink>/<searchLink fieldCode="MM" term="%22Antineoplastic+Agents+pharmacology%22">pharmacology</searchLink> <br /><searchLink fieldCode="MM" term="%22Complex+Mixtures%22">Complex Mixtures*</searchLink>/<searchLink fieldCode="MM" term="%22Complex+Mixtures+pharmacology%22">pharmacology</searchLink> <br /><searchLink fieldCode="MM" term="%22Complex+Mixtures%22">Complex Mixtures*</searchLink>/<searchLink fieldCode="MM" term="%22Complex+Mixtures+chemistry%22">chemistry</searchLink><br /><searchLink fieldCode="MH" term="%22Seeds%22">Seeds</searchLink>/<searchLink fieldCode="MH" term="%22Seeds+microbiology%22">microbiology</searchLink> ; <searchLink fieldCode="MH" term="%22Bacteria%22">Bacteria</searchLink>/<searchLink fieldCode="MH" term="%22Bacteria+drug+effects%22">drug effects</searchLink> ; <searchLink fieldCode="MH" term="%22Humans%22">Humans</searchLink> ; <searchLink fieldCode="MH" term="%22Cell+Line%2C+Tumor%22">Cell Line, Tumor</searchLink> ; <searchLink fieldCode="MH" term="%22Microbial+Sensitivity+Tests%22">Microbial Sensitivity Tests</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: This study presents a preliminary investigation into the antimicrobial, antibiofilm, and antiproliferative potential of Eremothecium coryli, an endophytic fungus isolated from the seeds of Basella alba L. The biological activities were evaluated using a crude extract-based screening study, employing the lyophilized cell-free supernatant (LCFS) of the fungal culture. Antibacterial and antibiofilm activities were assessed against selected multidrug-resistant bacterial pathogens, while antiproliferative activity was evaluated against breast cancer cell lines and a normal breast epithelial cell line. The LCFS demonstrated measurable antibacterial activity and significant biofilm-inhibition in a concentration-dependent manner. In cytotoxicity assays, selective growth inhibition of breast cancer cell lines was observed when compared with normal cells. Chemical profiling using Liquid Chromatography-Mass Spectrometry and Gas Chromatography-Mass Spectrometry enabled putative metabolite identification, revealing the presence of compounds previously reported to exhibit antimicrobial and anticancer activities. However, these identifications are tentative and based on spectral library matching. Overall, this preliminary investigation highlights the biological potential of E. coryli-derived metabolites. Further purification and validation are necessary to confirm compound identities, elucidate mechanisms of action, and assess in vivo relevance.<br /> (© 2026. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)
– Name: Abstract
  Label: Competing Interests
  Group: Ab
  Data: Declarations. Competing interests: The authors have no relevant financial or non-financial interests to disclose. Ethics declaration: Not applicable.
– Name: Ref
  Label: References
  Group: RefInfo
  Data: Ababutain IM, Aldosary SK, Aljuraifani AA et al (2021) Identification and antibacterial characterization of Endophytic Fungi from Artemisia sieberi. Int J Microbiol 2021:6651020. https://doi.org/10.1155/2021/6651020. (PMID: <searchLink fieldCode="PM" term="%2210%2E1155%2F2021%2F6651020337470877960065%22">10.1155/2021/6651020337470877960065)</searchLink><br />Brayton CF (1986) Dimethyl sulfoxide (DMSO): a review. Cornell Vet 76(1):61–90. (PMID: <searchLink fieldCode="PM" term="%223510103%22">3510103)</searchLink><br />Brito R, Silva G, Farias T, Ferreira P, Ferreira S (2017) Standardization of the safety level of the use of DMSO in viability assays in bacterial cells. MOL2NET, International Conference Series on Multidisciplinary Sciences http://sciforum.net/conference/mol2net-03 http:// https://doi.org/10.3390/MOL2NET-03-04980 .<br />Chatchawal C, Nualkaew N, Preeprame S, Porasuphatana S, Priprame A (2010) Physical and biological properties of mucilage from Basella alba L. stem and its gel formulation. Isan J Pharma Sci 6(3):104–112.<br />Clavel AJC, Villanueva JM, Yee JM (2019) Evaluation of the antimicrobial activity of ointments containing the ethanolic extract of Basella alba Linn leaves (Family basellaceae). [Bachelor's thesis, De La Salle Medical and Health Sciences Institute]. GreenPrints. https://greenprints.dlshsi.edu.ph/bsp/15/.<br />Do AD, Quang HP, Phan QK (2025) Probiotic cell-free supernatant as effective antimicrobials against Klebsiella pneumoniae and reduce antibiotic resistance development. Int Microbiol 28:623–632. https://doi.org/10.1007/s10123-024-00575-x. (PMID: <searchLink fieldCode="PM" term="%2210%2E1007%2Fs10123-024-00575-x39117894%22">10.1007/s10123-024-00575-x39117894)</searchLink><br />Ediriweera MK, Tennekoon KH, Samarakoon SR (2019) In vitro assays and techniques utilized in anticancer drug discovery. J Applied Toxicol 39(1):38–71. https://doi.org/10.1002/jat.3658. (PMID: <searchLink fieldCode="PM" term="%2210%2E1002%2Fjat%2E3658%22">10.1002/jat.3658)</searchLink><br />Hashem AH, Attia MS, Kandil EK, et al (2023) Bioactive compounds and biomedical applications of endophytic fungi: a recent review. Microb Cell Fact 6 22(1):107. https://doi.org/10.1186/s12934-023-02118-x . Erratum in: Microb Cell Fact. 22(1):122. 10.1186/s12934-023-02131-0.<br />Hema P, Murali M, Thriveni MC (2015) Phytochemical analysis and antibacterial activity of endophytic fungi isolated from Basella rubra L.,-A medicinal plant. J Pure Appl Microbiol 9(4):2971–2978.<br />Hridoy M, Gorapi MZH et al (2022) Putative anticancer compounds from plant derived endophytic fungi: a review. Molecules 27(1):296. https://doi.org/10.3390/molecules27010296. (PMID: <searchLink fieldCode="PM" term="%2210%2E3390%2Fmolecules27010296350115278746379%22">10.3390/molecules27010296350115278746379)</searchLink><br />Ibrahim TA, Ajongbolo KF, Aladekoyi G (2014) Phytochemical screening and antimicrobial activity of crude extracts of Basella alba and Helianthus annuus on selected food pathogens. J Microbiol Biotechnol 3(2):27–31.<br />Islam M, Rahi M, Jahangir CA, Rahman MH, Jerin I et al (2018) In vivo anticancer activity of Basella alba leaf and seed extracts against Ehrlich’s ascites carcinoma (EAC) cell line. Evi Based Complement Alternat Med 2018:1537896. https://doi.org/10.1155/2018/1537896. (PMID: <searchLink fieldCode="PM" term="%2210%2E1155%2F2018%2F1537896%22">10.1155/2018/1537896)</searchLink><br />Kamiloglu S, Sari G, Ozdal T, Capanoglu E (2020) Guidelines for cell viability assays. Food Front 1(3):332–349. (PMID: <searchLink fieldCode="PM" term="%2210%2E1002%2Ffft2%2E44%22">10.1002/fft2.44)</searchLink><br />Katoch M, Phull S, Vaid S, Singh S (2017) Diversity, Phylogeny, anticancer and antimicrobial potential of fungal endophytes associated with Monarda citriodora L. BioMed Central Microbiol 17(1):1–3. https://doi.org/10.1186/s12866-017-0961-2. (PMID: <searchLink fieldCode="PM" term="%2210%2E1186%2Fs12866-017-0961-2%22">10.1186/s12866-017-0961-2)</searchLink><br />Kaur N, Arora DS (2020) Prospecting the antimicrobial and antibiofilm potential of Chaetomium globosum an endophytic fungus from Moringa oleifera. AMB Express 10(1):1–3. https://doi.org/10.1186/s13568-020-01143-y. (PMID: <searchLink fieldCode="PM" term="%2210%2E1186%2Fs13568-020-01143-y%22">10.1186/s13568-020-01143-y)</searchLink><br />Kilari BP, Kotakadi VS, Penchalaneni J (2016) Anti-proliferative and apoptotic effects of Basella rubra (L.) Against 1, 2-Dimethyl Hydrazine-induced colon carcinogenesis in rats. Asian Pacific J Cancer Prevent 17(1) 73–80. http:// https://doi.org/10.7314/APJCP.2016.17.1.73 .<br />Kong YL, Tan JS, Goh CF (2025) Plant-Based Protein Fermentation Medium Optimization for Antimicrobial Cell-Free Supernatant Production by Lactiplantibacillus plantarum K014 Against Cutibacterium acnes Probiotics and Antimicro. Prot. https://doi.org/10.1007/s12602-025-10742-9. (PMID: <searchLink fieldCode="PM" term="%2210%2E1007%2Fs12602-025-10742-9%22">10.1007/s12602-025-10742-9)</searchLink><br />Morombaye SM, Kangogo M, Revathi G, Nyerere A, Ochora J (2018) Evaluation of the antimicrobial effect of Nepeta cataria and Basella alba against clinically resistant Acinetobacter baumannii in Nairobi Kenya. Adv Microbiol 8(10):790–803. https://doi.org/10.4236/aim.2018.810052. (PMID: <searchLink fieldCode="PM" term="%2210%2E4236%2Faim%2E2018%2E810052%22">10.4236/aim.2018.810052)</searchLink><br />Moutusi S, Buela Parivallal P, Prasannakumar MK, Kiranmayee P (2019) Morphological and molecular characterization of culturable leaf endophytic fungi from Malabar Spinach: The first report. Studies in Fungi 1:192–204. https://doi.org/10.5943/sif/4/1/21. (PMID: <searchLink fieldCode="PM" term="%2210%2E5943%2Fsif%2F4%2F1%2F21%22">10.5943/sif/4/1/21)</searchLink><br />Niu J, Li M, Wang Y (2024) Cell proliferation and cytotoxicity assays, the fundamental drug discovery. Int J Drug Discov Pharmacol 3(3):100013. https://doi.org/10.5394/ijddp.2024.100013. (PMID: <searchLink fieldCode="PM" term="%2210%2E5394%2Fijddp%2E2024%2E100013%22">10.5394/ijddp.2024.100013)</searchLink><br />Ogofure AG, Pelo SP, Green E (2024) Identification and assessment of secondary metabolites from three fungal endophytes of Solanum mauritianum against public health pathogens. Molecules 29(20):4924. https://doi.org/10.3390/molecules29204924. (PMID: <searchLink fieldCode="PM" term="%2210%2E3390%2Fmolecules292049243945929211510704%22">10.3390/molecules292049243945929211510704)</searchLink><br />Ogofure AG, Sebola TE, Green E (2025) Metabolic profile and bioactivity of fungal endophytes isolated from Crinum macowanii. BMC Comple Med Therapies 25(1):269. https://doi.org/10.1186/s12906-025-05011-9. (PMID: <searchLink fieldCode="PM" term="%2210%2E1186%2Fs12906-025-05011-9%22">10.1186/s12906-025-05011-9)</searchLink><br />Paul AK, Al Arif H, Seraj S, Nahar A, Nasrin D, Chowdhury MH et al (2011) A survey of plant items eaten by the low income groups of the rural population of Talbunia village in Bagerhat district, Bangladesh with an account of their folk medicinal applications. Adv Natural and App Sci 5(1):132–145.<br />Qayoom H, Alkhanani M, Almilaibary A, Alsagaby SA, Mir MA (2023) A network pharmacology-based investigation of brugine reveals its multi-target molecular mechanism against Breast Cancer. Med Oncol 40(7):202. https://doi.org/10.1007/s12032-023-02067-w. (PMID: <searchLink fieldCode="PM" term="%2210%2E1007%2Fs12032-023-02067-w37308611%22">10.1007/s12032-023-02067-w37308611)</searchLink><br />Riffiani R, Wada T, Shimomura N, Yamaguchi T, Aimi T (2021) An optimized method for high-quality DNA extraction medicinal fungi Mycoleptodonoides aitchisonii for whole genome sequencing. IOP Conf. Ser.: Earth Environ. Sci. 948: 012032. https://doi.org/10.1088/1755-1315/948/1/012032.<br />Rosdee S, Wisessombat S, Tayeh M, Malakul R, Phanaksri T, Sianglum W (2025) Antibacterial activity of the endophytic fungal extracts and synergistic effects of combinations of ethylenediaminetetraacetic acid (EDTA) against Pseudomonas aeruginosa and Escherichia coli. PeerJ 13:e19074. https://doi.org/10.7717/peerj.19074. (PMID: <searchLink fieldCode="PM" term="%2210%2E7717%2Fpeerj%2E190744006122511890036%22">10.7717/peerj.190744006122511890036)</searchLink><br />Saha M, Manasa AP, Prasannakumar MK, Pamidimukkala K (2024) Diversity pattern of cultivable endophytic microbes from various parts of two cultivars of indian spinach: Basella. Environ Eco Res 12(4):383–408. https://doi.org/10.13189/eer.2024.120405. (PMID: <searchLink fieldCode="PM" term="%2210%2E13189%2Feer%2E2024%2E120405%22">10.13189/eer.2024.120405)</searchLink><br />Saha M, Kiranmayee P (2024) First report on a wonder endophyte Coma circularis from Basella rubra L. stem: ahouse of pharmaceutically active compounds. The Microbe 4:100099. https://doi.org/10.1016/j.microb.2024.100099.<br />Sahoo S, Subban K, Chelliah J (2021) Diversity of marine macro-algicolous endophytic fungi and cytotoxic potential of Biscogniauxia petrensis metabolites against cancer cell lines. Front Microbiol 12:1472. https://doi.org/10.3389/fmicb.2021.650177. (PMID: <searchLink fieldCode="PM" term="%2210%2E3389%2Ffmicb%2E2021%2E650177%22">10.3389/fmicb.2021.650177)</searchLink><br />Sharma D, Misba L, Khan AU (2019) Antibiotics versus biofilm: an emerging battleground in microbial communities. Antimicrob Resist Infect Control 8:76. https://doi.org/10.1186/s13756-019-0533-3. (PMID: <searchLink fieldCode="PM" term="%2210%2E1186%2Fs13756-019-0533-3311311076524306%22">10.1186/s13756-019-0533-3311311076524306)</searchLink><br />Singh A, Singh DK, Kharwar RN, White JF, Gond SK (2021) Fungal endophytes as efficient sources of plant-derived bioactive compounds and their prospective applications in natural product drug discovery: insights, avenues and challenges. Microorganisms 9(1):197. https://doi.org/10.3390/microorganisms9010197. (PMID: <searchLink fieldCode="PM" term="%2210%2E3390%2Fmicroorganisms9010197334779107833388%22">10.3390/microorganisms9010197334779107833388)</searchLink><br />Sithranga Boopathy N, Kathiresan K (2010) Anticancer drugs from marine flora: an overview. J Oncol 2010:214186. https://doi.org/10.1155/2010/214186. (PMID: <searchLink fieldCode="PM" term="%2210%2E1155%2F2010%2F21418621461373%22">10.1155/2010/21418621461373)</searchLink><br />Sornsenee P, Chatatikun M, Mitsuwan W, Kongpol K, Kooltheat N (2021) Lyophilized cell-free supernatants of Lactobacillus isolates exhibited antibiofilm, antioxidant, and reduces nitric oxide activity in lipopolysaccharide-stimulated RAW 264.7 cells. PeerJ 9:e12586. https://doi.org/10.7717/peerj.12586. (PMID: <searchLink fieldCode="PM" term="%2210%2E7717%2Fpeerj%2E12586349092858641486%22">10.7717/peerj.12586349092858641486)</searchLink><br />Techaoei S, Jirayuthcharoenkul C, Jarmkom K et al (2020) Chemical evaluation and antibacterial activity of novel bioactive compounds from endophytic fungi in Nelumbo nucifera. Saudi J Biol Sci 27(11):2883–2889. https://doi.org/10.1016/j.sjbs.2020.08.037. (PMID: <searchLink fieldCode="PM" term="%2210%2E1016%2Fj%2Esjbs%2E2020%2E08%2E037331008437569113%22">10.1016/j.sjbs.2020.08.037331008437569113)</searchLink><br />Techaoei S, Jarmkom K, Dumrongphuttidecha T, Khobjai W (2021a) Evaluation of the stability and antibacterial activity of crude extracts of hydro-endophytic fungi. J Advan Pharma Technol Res 12(1):61. https://doi.org/10.4103/japtr.JAPTR_114_20. (PMID: <searchLink fieldCode="PM" term="%2210%2E4103%2Fjaptr%2EJAPTR%26#95%3B114%26#95%3B20%22">10.4103/japtr.JAPTR_114_20)</searchLink><br />Techaoei S, Jarmkom K, Dumrongphuttidecha T, Khobjai W (2021b) Evaluation of the stability and antibacterial activity of crude extracts of hydro-endophytic fungi. J Adv Pharm Technol Res 12(1):61–66. https://doi.org/10.4103/japtr.JAPTR_114_20. (PMID: <searchLink fieldCode="PM" term="%2210%2E4103%2Fjaptr%2EJAPTR%26#95%3B114%26#95%3B20335323577832182%22">10.4103/japtr.JAPTR_114_20335323577832182)</searchLink><br />Wen J, Okyere SK, Wang S et al (2022) Endophytic fungi: an effective alternative source of plant-derived bioactive compounds for pharmacological studies. J Fungi (Basel) 8(2):205. https://doi.org/10.3390/jof8020205. (PMID: <searchLink fieldCode="PM" term="%2210%2E3390%2Fjof8020205352059598877053%22">10.3390/jof8020205352059598877053)</searchLink>
– Name: SubjectMinor
  Label: Contributed Indexing
  Group:
  Data: <i>Keywords: </i>Biofilm; Breast cancer cell lines; Initial cell attachment; Mass spectrometry; Multidrug-resistant bacteria
– Name: NumberCAS
  Label: Substance Nomenclature
  Group: ID
  Data: 0 (Anti-Infective Agents)<br />0 (Anti-Bacterial Agents)<br />0 (Antineoplastic Agents)<br />0 (Complex Mixtures)
– Name: DateEntry
  Label: Entry Date(s)
  Group: Date
  Data: <i>Date Created: </i>20260801 <i>Date Completed: </i>20260801 <i>Latest Revision: </i>20260905
– Name: DateUpdate
  Label: Update Code
  Group: Date
  Data: 20260905
– Name: DOI
  Label: DOI
  Group: ID
  Data: 10.1007/s10482-026-02368-w
– Name: AN
  Label: PMID
  Group: ID
  Data: 42541521
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=cmedm&AN=42541521
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1007/s10482-026-02368-w
    Languages:
      – Code: eng
        Text: English
    Subjects:
      – SubjectFull: Seeds microbiology
        Type: general
      – SubjectFull: Bacteria drug effects
        Type: general
      – SubjectFull: Humans
        Type: general
      – SubjectFull: Cell Line, Tumor
        Type: general
      – SubjectFull: Microbial Sensitivity Tests
        Type: general
      – SubjectFull: Biofilms drug effects
        Type: general
      – SubjectFull: Cell Proliferation drug effects
        Type: general
      – SubjectFull: Endophytes chemistry
        Type: general
      – SubjectFull: Endophytes isolation & purification
        Type: general
      – SubjectFull: Ascomycota chemistry
        Type: general
      – SubjectFull: Anti-Infective Agents pharmacology
        Type: general
      – SubjectFull: Anti-Infective Agents chemistry
        Type: general
      – SubjectFull: Anti-Infective Agents isolation & purification
        Type: general
      – SubjectFull: Anti-Bacterial Agents pharmacology
        Type: general
      – SubjectFull: Antineoplastic Agents pharmacology
        Type: general
      – SubjectFull: Complex Mixtures pharmacology
        Type: general
      – SubjectFull: Complex Mixtures chemistry
        Type: general
    Titles:
      – TitleFull: A preliminary investigation of antimicrobial, antibiofilm, and antiproliferative properties of a seed-derived endophytic fungus (Eremothecium coryli) from Basella alba L.: a crude extract-based screening study.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Saha M
      – PersonEntity:
          Name:
            NameFull: Pamidimukkala K
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 08
              Text: 2026 Aug 01
              Type: published
              Y: 2026
          Identifiers:
            – Type: issn-electronic
              Value: 1572-9699
          Numbering:
            – Type: volume
              Value: 119
            – Type: issue
              Value: 9
          Titles:
            – TitleFull: Antonie van Leeuwenhoek
              Type: main
ResultId 1