Academic Journal
Προβολή σε EDS

Memory impairments induced by neonatal excitotoxicity are associated with altered expression of NMDA receptor subunits NR2A and NR2B.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος: Memory impairments induced by neonatal excitotoxicity are associated with altered expression of NMDA receptor subunits NR2A and NR2B.
Συγγραφείς: Castañeda-Cabral JL; Laboratorio de Biología de la Neurotransmisión, México. Electronic address: jluis.castaneda@academicos.udg.mx., López-Pérez SJ; Laboratorio de Neuroquímica, México., Contreras-Marín S; Laboratorio de Biología de la Neurotransmisión, México., Castro-Torres RD; Laboratorio de Neurobiotecnología, Departamento de Biología Celular y Molecular, Centro Universitario de Ciencias Biológicas y Agropecuarias (CUCBA), Universidad de Guadalajara, Zapopan, Mexico., Ureña-Guerrero ME; Laboratorio de Biología de la Neurotransmisión, México. Electronic address: murena@cucba.udg.mx.
Πηγή: Physiology & behavior [Physiol Behav] 2026 Oct 15; Vol. 315, pp. 115456. Date of Electronic Publication: 2026 Jul 28.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Elsevier Science Country of Publication: United States NLM ID: 0151504 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-507X (Electronic) Linking ISSN: 00319384 NLM ISO Abbreviation: Physiol Behav Subsets: MEDLINE
Imprint Name(s): Publication: New York NY : Elsevier Science
Original Publication: Oxford, Eng., Long Island City, Pergamon Press.
Ιατρικοί όροι (MeSH): Receptors, N-Methyl-D-Aspartate*/metabolism , Memory Disorders*/chemically induced , Memory Disorders*/metabolism , Sodium Glutamate*/toxicity , Brain*/metabolism , Brain*/drug effects, Maze Learning/drug effects ; Maze Learning/physiology ; Recognition, Psychology/drug effects ; Recognition, Psychology/physiology ; Animals ; Male ; Animals, Newborn ; Rats ; Rats, Wistar ; Disease Models, Animal
Περίληψη: N-methyl-d-aspartate (NMDA) receptors are typically composed of two NR1 subunits and two NR2 (A-D) subunits. These receptors are overactivated during excitotoxicity, which can alter the expression of their subunits. Neonatal treatment with monosodium glutamate (MSG) induces a long-lasting neurodegenerative process triggered by excitotoxicity and involving multiple alterations in neurotransmission systems. This study aimed to determine whether neonatal MSG treatment modifies the protein expression of NR1, NR2A, and NR2B subunits, and whether these changes are associated with memory impairments in adulthood. Male neonatal rats received 4 g/kg MSG subcutaneously on postnatal days (PD) 1, 3, 5, and 7. At PD 60, protein expression of NR1, NR2A, and NR2B was assessed in the cerebral motor cortex (CMC), striatum (STR), hippocampus (Hp), and entorhinal cortex (EC) using Western blot. Memory performance was evaluated between PD 55 and PD 60 using the Barnes maze and novel object recognition tests. NR1 expression did not show significant changes in any brain region analyzed. NR2A expression was significantly increased in the CMC (p< 0.001) and decreased in the STR (p< 0.01). In contrast, NR2B expression was reduced in the CMC (p< 0.05) and increased in the STR (p< 0.01) and Hp (p< 0.05). MSG-treated animals exhibited impairments in short-term spatial and working memory. These findings indicate that neonatal excitotoxicity induced by MSG leads to long-term, region-specific alterations in NMDA receptor subunit composition, which may disrupt glutamatergic signaling and contribute to cognitive deficits in adulthood.
(Copyright © 2026 Elsevier Inc. All rights reserved.)
Competing Interests: Declaration of competing interest The authors declare no competing financial interests.
Contributed Indexing: Keywords: Excitotoxicity; Memory; Monosodium glutamate; NMDA receptor
Substance Nomenclature: 0 (Receptors, N-Methyl-D-Aspartate)
0 (NR2B NMDA receptor)
0 (NR2A NMDA receptor)
W81N5U6R6U (Sodium Glutamate)
0 (NR1 NMDA receptor)
Entry Date(s): Date Created: 20260728 Date Completed: 20260807 Latest Revision: 20260807
Update Code: 20260808
DOI: 10.1016/j.physbeh.2026.115456
PMID: 42520952
Βάση Δεδομένων: MEDLINE
FullText Text:
  Availability: 0
CustomLinks:
  – Url: https://www.doi.org/10.1016/j.physbeh.2026.115456?
    Name: ScienceDirect (all content) (s7799221)
    Category: fullText
    Text: View record from ScienceDirect
    MouseOverText: View record from ScienceDirect
  – Url: https://resolver.ebsco.com/c/fiv2js/result?sid=EBSCO:cmedm&genre=article&issn=1873507X&ISBN=&volume=315&issue=&date=20261015&spage=115456&pages=115456&title=Physiology & behavior&atitle=Memory%20impairments%20induced%20by%20neonatal%20excitotoxicity%20are%20associated%20with%20altered%20expression%20of%20NMDA%20receptor%20subunits%20NR2A%20and%20NR2B.&aulast=Casta%C3%B1eda-Cabral%20JL&id=DOI:10.1016/j.physbeh.2026.115456
    Name: Full Text Finder (for New FTF UI) (ns324271)
    Category: fullText
    Text: Full Text Finder
    MouseOverText: Full Text Finder
Header DbId: cmedm
DbLabel: MEDLINE
An: 42520952
AccessLevel: 3
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Memory impairments induced by neonatal excitotoxicity are associated with altered expression of NMDA receptor subunits NR2A and NR2B.
– Name: Author
  Label: Authors
  Group: Au
  Data: &lt;searchLink fieldCode=&quot;AU&quot; term=&quot;%22Casta&#241;eda-Cabral+JL%22&quot;&gt;Casta&#241;eda-Cabral JL&lt;/searchLink&gt;; Laboratorio de Biolog&#237;a de la Neurotransmisi&#243;n, M&#233;xico. Electronic address: jluis.castaneda@academicos.udg.mx.&lt;br /&gt;&lt;searchLink fieldCode=&quot;AU&quot; term=&quot;%22L&#243;pez-P&#233;rez+SJ%22&quot;&gt;L&#243;pez-P&#233;rez SJ&lt;/searchLink&gt;; Laboratorio de Neuroqu&#237;mica, M&#233;xico.&lt;br /&gt;&lt;searchLink fieldCode=&quot;AU&quot; term=&quot;%22Contreras-Mar&#237;n+S%22&quot;&gt;Contreras-Mar&#237;n S&lt;/searchLink&gt;; Laboratorio de Biolog&#237;a de la Neurotransmisi&#243;n, M&#233;xico.&lt;br /&gt;&lt;searchLink fieldCode=&quot;AU&quot; term=&quot;%22Castro-Torres+RD%22&quot;&gt;Castro-Torres RD&lt;/searchLink&gt;; Laboratorio de Neurobiotecnolog&#237;a, Departamento de Biolog&#237;a Celular y Molecular, Centro Universitario de Ciencias Biol&#243;gicas y Agropecuarias (CUCBA), Universidad de Guadalajara, Zapopan, Mexico.&lt;br /&gt;&lt;searchLink fieldCode=&quot;AU&quot; term=&quot;%22Ure&#241;a-Guerrero+ME%22&quot;&gt;Ure&#241;a-Guerrero ME&lt;/searchLink&gt;; Laboratorio de Biolog&#237;a de la Neurotransmisi&#243;n, M&#233;xico. Electronic address: murena@cucba.udg.mx.
– Name: TitleSource
  Label: Source
  Group: Src
  Data: &lt;searchLink fieldCode=&quot;JN&quot; term=&quot;%220151504%22&quot;&gt;Physiology &amp; behavior&lt;/searchLink&gt; [Physiol Behav] 2026 Oct 15; Vol. 315, pp. 115456. &lt;i&gt;Date of Electronic Publication: &lt;/i&gt;2026 Jul 28.
– Name: TypePub
  Label: Publication Type
  Group: TypPub
  Data: Journal Article
– Name: Language
  Label: Language
  Group: Lang
  Data: English
– Name: TitleSource
  Label: Journal Info
  Group: Src
  Data: &lt;i&gt;Publisher: &lt;/i&gt;&lt;searchLink fieldCode=&quot;PB&quot; term=&quot;%22Elsevier+Science%22&quot;&gt;Elsevier Science &lt;/searchLink&gt;&lt;i&gt;Country of Publication: &lt;/i&gt;United States &lt;i&gt;NLM ID: &lt;/i&gt;0151504 &lt;i&gt;Publication Model: &lt;/i&gt;Print-Electronic &lt;i&gt;Cited Medium: &lt;/i&gt;Internet &lt;i&gt;ISSN: &lt;/i&gt;1873-507X (Electronic) &lt;i&gt;Linking ISSN: &lt;/i&gt;&lt;searchLink fieldCode=&quot;IS&quot; term=&quot;%2200319384%22&quot;&gt;00319384 &lt;/searchLink&gt;&lt;i&gt;NLM ISO Abbreviation: &lt;/i&gt;Physiol Behav &lt;i&gt;Subsets: &lt;/i&gt;MEDLINE
– Name: PublisherInfo
  Label: Imprint Name(s)
  Group: PubInfo
  Data: &lt;i&gt;Publication&lt;/i&gt;: New York NY : Elsevier Science&lt;br /&gt;&lt;i&gt;Original Publication&lt;/i&gt;: Oxford, Eng., Long Island City, Pergamon Press.
– Name: SubjectMESH
  Label: MeSH Terms
  Group: Su
  Data: &lt;searchLink fieldCode=&quot;MM&quot; term=&quot;%22Receptors%2C+N-Methyl-D-Aspartate%22&quot;&gt;Receptors, N-Methyl-D-Aspartate*&lt;/searchLink&gt;/&lt;searchLink fieldCode=&quot;MM&quot; term=&quot;%22Receptors%2C+N-Methyl-D-Aspartate+metabolism%22&quot;&gt;metabolism&lt;/searchLink&gt; &lt;br /&gt;&lt;searchLink fieldCode=&quot;MM&quot; term=&quot;%22Memory+Disorders%22&quot;&gt;Memory Disorders*&lt;/searchLink&gt;/&lt;searchLink fieldCode=&quot;MM&quot; term=&quot;%22Memory+Disorders+chemically+induced%22&quot;&gt;chemically induced&lt;/searchLink&gt; &lt;br /&gt;&lt;searchLink fieldCode=&quot;MM&quot; term=&quot;%22Memory+Disorders%22&quot;&gt;Memory Disorders*&lt;/searchLink&gt;/&lt;searchLink fieldCode=&quot;MM&quot; term=&quot;%22Memory+Disorders+metabolism%22&quot;&gt;metabolism&lt;/searchLink&gt; &lt;br /&gt;&lt;searchLink fieldCode=&quot;MM&quot; term=&quot;%22Sodium+Glutamate%22&quot;&gt;Sodium Glutamate*&lt;/searchLink&gt;/&lt;searchLink fieldCode=&quot;MM&quot; term=&quot;%22Sodium+Glutamate+toxicity%22&quot;&gt;toxicity&lt;/searchLink&gt; &lt;br /&gt;&lt;searchLink fieldCode=&quot;MM&quot; term=&quot;%22Brain%22&quot;&gt;Brain*&lt;/searchLink&gt;/&lt;searchLink fieldCode=&quot;MM&quot; term=&quot;%22Brain+metabolism%22&quot;&gt;metabolism&lt;/searchLink&gt; &lt;br /&gt;&lt;searchLink fieldCode=&quot;MM&quot; term=&quot;%22Brain%22&quot;&gt;Brain*&lt;/searchLink&gt;/&lt;searchLink fieldCode=&quot;MM&quot; term=&quot;%22Brain+drug+effects%22&quot;&gt;drug effects&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;MH&quot; term=&quot;%22Maze+Learning%22&quot;&gt;Maze Learning&lt;/searchLink&gt;/&lt;searchLink fieldCode=&quot;MH&quot; term=&quot;%22Maze+Learning+drug+effects%22&quot;&gt;drug effects&lt;/searchLink&gt; ; &lt;searchLink fieldCode=&quot;MH&quot; term=&quot;%22Maze+Learning%22&quot;&gt;Maze Learning&lt;/searchLink&gt;/&lt;searchLink fieldCode=&quot;MH&quot; term=&quot;%22Maze+Learning+physiology%22&quot;&gt;physiology&lt;/searchLink&gt; ; &lt;searchLink fieldCode=&quot;MH&quot; term=&quot;%22Recognition%2C+Psychology%22&quot;&gt;Recognition, Psychology&lt;/searchLink&gt;/&lt;searchLink fieldCode=&quot;MH&quot; term=&quot;%22Recognition%2C+Psychology+drug+effects%22&quot;&gt;drug effects&lt;/searchLink&gt; ; &lt;searchLink fieldCode=&quot;MH&quot; term=&quot;%22Recognition%2C+Psychology%22&quot;&gt;Recognition, Psychology&lt;/searchLink&gt;/&lt;searchLink fieldCode=&quot;MH&quot; term=&quot;%22Recognition%2C+Psychology+physiology%22&quot;&gt;physiology&lt;/searchLink&gt; ; &lt;searchLink fieldCode=&quot;MH&quot; term=&quot;%22Animals%22&quot;&gt;Animals&lt;/searchLink&gt; ; &lt;searchLink fieldCode=&quot;MH&quot; term=&quot;%22Male%22&quot;&gt;Male&lt;/searchLink&gt; ; &lt;searchLink fieldCode=&quot;MH&quot; term=&quot;%22Animals%2C+Newborn%22&quot;&gt;Animals, Newborn&lt;/searchLink&gt; ; &lt;searchLink fieldCode=&quot;MH&quot; term=&quot;%22Rats%22&quot;&gt;Rats&lt;/searchLink&gt; ; &lt;searchLink fieldCode=&quot;MH&quot; term=&quot;%22Rats%2C+Wistar%22&quot;&gt;Rats, Wistar&lt;/searchLink&gt; ; &lt;searchLink fieldCode=&quot;MH&quot; term=&quot;%22Disease+Models%2C+Animal%22&quot;&gt;Disease Models, Animal&lt;/searchLink&gt;
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: N-methyl-d-aspartate (NMDA) receptors are typically composed of two NR1 subunits and two NR2 (A-D) subunits. These receptors are overactivated during excitotoxicity, which can alter the expression of their subunits. Neonatal treatment with monosodium glutamate (MSG) induces a long-lasting neurodegenerative process triggered by excitotoxicity and involving multiple alterations in neurotransmission systems. This study aimed to determine whether neonatal MSG treatment modifies the protein expression of NR1, NR2A, and NR2B subunits, and whether these changes are associated with memory impairments in adulthood. Male neonatal rats received 4 g/kg MSG subcutaneously on postnatal days (PD) 1, 3, 5, and 7. At PD 60, protein expression of NR1, NR2A, and NR2B was assessed in the cerebral motor cortex (CMC), striatum (STR), hippocampus (Hp), and entorhinal cortex (EC) using Western blot. Memory performance was evaluated between PD 55 and PD 60 using the Barnes maze and novel object recognition tests. NR1 expression did not show significant changes in any brain region analyzed. NR2A expression was significantly increased in the CMC (p&amp;lt; 0.001) and decreased in the STR (p&amp;lt; 0.01). In contrast, NR2B expression was reduced in the CMC (p&amp;lt; 0.05) and increased in the STR (p&amp;lt; 0.01) and Hp (p&amp;lt; 0.05). MSG-treated animals exhibited impairments in short-term spatial and working memory. These findings indicate that neonatal excitotoxicity induced by MSG leads to long-term, region-specific alterations in NMDA receptor subunit composition, which may disrupt glutamatergic signaling and contribute to cognitive deficits in adulthood.&lt;br /&gt; (Copyright &#169; 2026 Elsevier Inc. All rights reserved.)
– Name: Abstract
  Label: Competing Interests
  Group: Ab
  Data: Declaration of competing interest The authors declare no competing financial interests.
– Name: SubjectMinor
  Label: Contributed Indexing
  Group:
  Data: &lt;i&gt;Keywords: &lt;/i&gt;Excitotoxicity; Memory; Monosodium glutamate; NMDA receptor
– Name: NumberCAS
  Label: Substance Nomenclature
  Group: ID
  Data: 0 (Receptors, N-Methyl-D-Aspartate)&lt;br /&gt;0 (NR2B NMDA receptor)&lt;br /&gt;0 (NR2A NMDA receptor)&lt;br /&gt;W81N5U6R6U (Sodium Glutamate)&lt;br /&gt;0 (NR1 NMDA receptor)
– Name: DateEntry
  Label: Entry Date(s)
  Group: Date
  Data: &lt;i&gt;Date Created: &lt;/i&gt;20260728 &lt;i&gt;Date Completed: &lt;/i&gt;20260807 &lt;i&gt;Latest Revision: &lt;/i&gt;20260807
– Name: DateUpdate
  Label: Update Code
  Group: Date
  Data: 20260808
– Name: DOI
  Label: DOI
  Group: ID
  Data: 10.1016/j.physbeh.2026.115456
– Name: AN
  Label: PMID
  Group: ID
  Data: 42520952
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=cmedm&AN=42520952
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1016/j.physbeh.2026.115456
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        StartPage: 115456
    Subjects:
      – SubjectFull: Maze Learning drug effects
        Type: general
      – SubjectFull: Maze Learning physiology
        Type: general
      – SubjectFull: Recognition, Psychology drug effects
        Type: general
      – SubjectFull: Recognition, Psychology physiology
        Type: general
      – SubjectFull: Animals
        Type: general
      – SubjectFull: Male
        Type: general
      – SubjectFull: Animals, Newborn
        Type: general
      – SubjectFull: Rats
        Type: general
      – SubjectFull: Rats, Wistar
        Type: general
      – SubjectFull: Disease Models, Animal
        Type: general
      – SubjectFull: Receptors, N-Methyl-D-Aspartate metabolism
        Type: general
      – SubjectFull: Memory Disorders chemically induced
        Type: general
      – SubjectFull: Memory Disorders metabolism
        Type: general
      – SubjectFull: Sodium Glutamate toxicity
        Type: general
      – SubjectFull: Brain metabolism
        Type: general
      – SubjectFull: Brain drug effects
        Type: general
    Titles:
      – TitleFull: Memory impairments induced by neonatal excitotoxicity are associated with altered expression of NMDA receptor subunits NR2A and NR2B.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Castañeda-Cabral JL
      – PersonEntity:
          Name:
            NameFull: López-Pérez SJ
      – PersonEntity:
          Name:
            NameFull: Contreras-Marín S
      – PersonEntity:
          Name:
            NameFull: Castro-Torres RD
      – PersonEntity:
          Name:
            NameFull: Ureña-Guerrero ME
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 15
              M: 10
              Text: 2026 Oct 15
              Type: published
              Y: 2026
          Identifiers:
            – Type: issn-electronic
              Value: 1873-507X
          Numbering:
            – Type: volume
              Value: 315
          Titles:
            – TitleFull: Physiology & behavior
              Type: main
ResultId 1