Academic Journal

Wastewater Surveillance of Aichi Virus in Baltimore.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος: Wastewater Surveillance of Aichi Virus in Baltimore.
Συγγραφείς: Nwaubani DA; Center of Research Excellence in Wastewater Based Epidemiology, Morgan State University, Baltimore, MD 21251, USA.; Bioenvironmental Science Program, Morgan State University, Baltimore, MD 21251, USA., Baral R; Center of Research Excellence in Wastewater Based Epidemiology, Morgan State University, Baltimore, MD 21251, USA., Solomon T; Center of Research Excellence in Wastewater Based Epidemiology, Morgan State University, Baltimore, MD 21251, USA., Ali M; Center of Research Excellence in Wastewater Based Epidemiology, Morgan State University, Baltimore, MD 21251, USA., Moharrery T; Center of Research Excellence in Wastewater Based Epidemiology, Morgan State University, Baltimore, MD 21251, USA., Sherchan SP; Center of Research Excellence in Wastewater Based Epidemiology, Morgan State University, Baltimore, MD 21251, USA.; Bioenvironmental Science Program, Morgan State University, Baltimore, MD 21251, USA.
Πηγή: Pathogens (Basel, Switzerland) [Pathogens] 2026 Jul 10; Vol. 15 (7). Date of Electronic Publication: 2026 Jul 10.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101596317 Publication Model: Electronic Cited Medium: Internet ISSN: 2076-0817 (Electronic) Linking ISSN: 20760817 NLM ISO Abbreviation: Pathogens Subsets: MEDLINE
Imprint Name(s): Original Publication: Basel, Switzerland : MDPI AG, 2012-
Ιατρικοί όροι (MeSH): Kobuvirus*/isolation & purification , Kobuvirus*/genetics , Wastewater*/virology , Picornaviridae Infections*/virology , Picornaviridae Infections*/epidemiology, Baltimore/epidemiology ; RNA, Viral/genetics ; Seasons ; Environmental Monitoring ; Humans
Περίληψη: This study established long-term wastewater surveillance of Aichi virus (AiV) in Maryland. AiV, a member of the Kobuvirus genus associated with acute gastroenteritis, has established itself as an integral marker for wastewater-based monitoring; however, two key research questions remain unaddressed for the Baltimore metropolitan area: (1) whether AiV is consistently detectable in municipal wastewater throughout the year, and (2) whether its concentrations exhibit a measurable seasonal pattern. To address these hypotheses, influent samples were collected on a weekly basis from WWTP-A and WWTP-B from January to December 2023 (with grab sampling conducted at WWTP-A and automated collection deployed for the influent sampling of the water treatment plant B). All samples (n = 51) were subjected to PEG 8000 concentration, RNA extraction, cDNA synthesis, and RT-qPCR quantification. We observed AiV RNA in 94.12% of the samples from both facilities (25/51 at WWTP-A and 23/51 at WWTP-B) with concentrations that ranged from 2.5 to 3.63 log10 gc/L and a seasonal pattern showing consistent declines: loads for WWTP-A declining from winter (3.58 log10 gc/L) to fall (2.56) and for WWTP-B from winter (3.28 log10 gc/L) to fall (2.31). The year-round constant AiV presence provides a strong basis for its use as a stable viral marker within wastewater-based epidemiology efforts.
Contributed Indexing: Keywords: Aichi virus; Baltimore; RT-qPCR; enteric viruses; fecal contamination indicators; physicochemical parameters; wastewater-based epidemiology
Substance Nomenclature: 0 (Wastewater)
0 (RNA, Viral)
Entry Date(s): Date Created: 20260728 Date Completed: 20260729 Latest Revision: 20260731
Update Code: 20260731
PubMed Central ID: PMC13414499
DOI: 10.3390/pathogens15070728
PMID: 42515055
Βάση Δεδομένων: MEDLINE