Academic Journal
Baseline Characterization of the Gut Microbiota of Field and Colony Populations of Phlebotomus tobbi and Preliminary Assessment of the Anti-Leishmanial Activity of Cultivable Bacteria.
| Τίτλος: | Baseline Characterization of the Gut Microbiota of Field and Colony Populations of Phlebotomus tobbi and Preliminary Assessment of the Anti-Leishmanial Activity of Cultivable Bacteria. |
|---|---|
| Συγγραφείς: | Karakuş M; Department of Medical Microbiology, Hamidiye Faculty of Medicine, University of Health Sciences, Istanbul 34668, Türkiye., Yılmaz A; VERG Laboratories, Department of Biology, Faculty of Science, Hacettepe University, Beytepe, Ankara 06800, Türkiye., Okbay M; VERG Laboratories, Department of Biology, Faculty of Science, Hacettepe University, Beytepe, Ankara 06800, Türkiye., Pekağırbaş M; Department of Parasitology, Faculty of Veterinary Medicine, Aydın Adnan Menderes University, Aydın 09016, Türkiye., Erisoz Kasap O; VERG Laboratories, Department of Biology, Faculty of Science, Hacettepe University, Beytepe, Ankara 06800, Türkiye. |
| Πηγή: | Pathogens (Basel, Switzerland) [Pathogens] 2026 Jun 23; Vol. 15 (7). Date of Electronic Publication: 2026 Jun 23. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101596317 Publication Model: Electronic Cited Medium: Internet ISSN: 2076-0817 (Electronic) Linking ISSN: 20760817 NLM ISO Abbreviation: Pathogens Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Basel, Switzerland : MDPI AG, 2012- |
| Ιατρικοί όροι (MeSH): | Phlebotomus*/microbiology , Phlebotomus*/parasitology , Bacteria*/classification , Bacteria*/isolation & purification , Bacteria*/genetics , Gastrointestinal Microbiome* , Leishmania infantum*, RNA, Ribosomal, 16S/genetics ; Animals ; Female ; Male ; Phylogeny |
| Περίληψη: | Sand fly midgut microbiota plays a critical role in shaping Leishmania development and vector competence, yet functional evidence from natural vector populations remains limited. In this study, sand flies were collected between 2020 and 2022 in Cukurova region, Türkiye to characterize the gut bacterial composition of Phlebotomus tobbi and evaluate the anti-leishmanial potential of cultivable isolates. A total of 1739 sand flies were captured (878 females, 861 males), of which Ph. tobbi was the predominant species (n = 1312). 16S rRNA amplicon sequencing (V4-V6) showed that the gut microbiota was dominated by Proteobacteria, with Erwinia aphidicola/persicina representing the most abundant species across all analyzed groups. Fourteen cultivable bacterial species were identified by MALDI-TOF MS, including Serratia liquefaciens, Pantoea agglomerans, and Micrococcus luteus. Functional XTT assays against Leishmania infantum promastigotes demonstrated variable inhibitory activity among isolates. The strongest leishmanicidal effects were observed with S. liquefaciens (32.3%) and M. luteus (28.8%). Morphological examination confirmed promastigote rounding and cell death in isolates showing >25% activity. These findings define the gut bacterial landscape of Ph. tobbi in an endemic region and identify bacterial taxa with in vitro anti-leishmanial activity, highlighting their potential for future microbiota-based or paratransgenic control strategies. |
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| Grant Information: | 218Z030 TUBİTAK |
| Contributed Indexing: | Keywords: 16S rRNA sequencing; Leishmania infantum; MALDI-TOF; Phlebotomus tobbi; leishmanicidal activity; midgut microbiota; paratransgenesis |
| Substance Nomenclature: | 0 (RNA, Ribosomal, 16S) |
| Entry Date(s): | Date Created: 20260728 Date Completed: 20260729 Latest Revision: 20260813 |
| Update Code: | 20260814 |
| PubMed Central ID: | PMC13416292 |
| DOI: | 10.3390/pathogens15070658 |
| PMID: | 42514985 |
| Βάση Δεδομένων: | MEDLINE |
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