Academic Journal
Mobile genetic elements-driven partitions of mega-plasmids resistome in Salmonella Infantis.
| Τίτλος: | Mobile genetic elements-driven partitions of mega-plasmids resistome in Salmonella Infantis. |
|---|---|
| Συγγραφείς: | Zhang Y; Produce Safety and Microbiology Research Unit, U.S. Department of Agriculture, Agricultural Research Service, Western Regional Research Center, Albany, California, USA., Chu M; Produce Safety and Microbiology Research Unit, U.S. Department of Agriculture, Agricultural Research Service, Western Regional Research Center, Albany, California, USA., Liao Y-T; Produce Safety and Microbiology Research Unit, U.S. Department of Agriculture, Agricultural Research Service, Western Regional Research Center, Albany, California, USA., Wu VCH; Produce Safety and Microbiology Research Unit, U.S. Department of Agriculture, Agricultural Research Service, Western Regional Research Center, Albany, California, USA. |
| Πηγή: | Microbiology spectrum [Microbiol Spectr] 2026 Sep; Vol. 14 (9), pp. e0182725. Date of Electronic Publication: 2026 Jul 23. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: ASM Press Country of Publication: United States NLM ID: 101634614 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2165-0497 (Electronic) Linking ISSN: 21650497 NLM ISO Abbreviation: Microbiol Spectr Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Washington, DC : ASM Press, 2013- |
| Ιατρικοί όροι (MeSH): | Salmonella enterica*/genetics , Salmonella enterica*/drug effects , Plasmids*/genetics , Interspersed Repetitive Sequences*, Virulence Factors/genetics ; Anti-Bacterial Agents/pharmacology ; Salmonella Infections/microbiology ; Drug Resistance, Bacterial/genetics ; Salmonella/genetics ; Drug Resistance, Multiple, Bacterial/genetics ; Gene Transfer, Horizontal ; Humans ; Genome, Bacterial |
| Περίληψη: | Salmonella enterica serovar Infantis (S. Infantis) becomes the primary pathogen among the top Salmonella serotypes, contributing to numerous cases of foodborne illness annually in the United States. S. Infantis infection has spread rapidly worldwide, especially the clones with pESI-like plasmids. However, the underlying mechanisms regarding the transmission of S. Infantis, particularly mobile genetic elements (MGEs), mediated horizontal gene transfer, are limited. The objective of this study was to evaluate the relationship, if any, among MGEs, antibiotic-resistant genes (ARGs), and virulence factors (VFs) within S. Infantis via genomic analysis. A total of 91 S. Infantis complete genomes with high sequencing quality were selected for downstream bioinformatic analysis. The results showed that the majority of VFs were located in the bacterial chromosomes, while most ARGs were carried by S. Infantis mega-plasmids in an MGE-favored manner. Integrons and transposons were closely associated with certain ARGs, but prophages within mega-plasmids displayed a diverse ARG profile. Collectively, MGE-mediated horizontal gene transfer might lead to ARG acquisition by mega-plasmids, subsequently contributing to the resistome of S. Infantis. Our findings provide insights into the development of MGE-associated resistome in S. Infantis that could inform more effective prevention and intervention strategies to control this pathogen, further ensuring public health and safety.IMPORTANCEThe rapid emergence and transmission of antibiotic-resistant foodborne pathogens pose a significant risk to public health, necessitating the discovery of underlying mechanisms to control multidrug-resistant pathogens. Salmonella enterica serovar Infantis (S. Infantis) has become a pathogen of clinical and epidemiological relevance in recent years, ranking as the top prevalent serovar associated with foodborne illnesses and exhibiting resistance to several antibiotics. The current investigation of multidrug resistance (MDR) S. Infantis strains primarily emphasized the presence of mega-plasmids. However, the question of how mega-plasmids contribute to the transmission of antibiotic-resistant genes (ARG) is unaddressed. Utilizing the genomic characterization of S. Infantis complete genomes with high quality, our study revealed that the resistome of S. Infantis mega-plasmids-the primary ARG reservoirs of S. Infantis-followed a specific pattern of mobile genetic elements (MGEs). Monitoring the spread of MGE-carried ARGs within mega-plasmids should be considered in future surveillance. |
| Competing Interests: | The authors declare no conflict of interest. |
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| Grant Information: | 2030-42000-055-000-D Agricultural Research Service |
| Contributed Indexing: | Keywords: Salmonella Infantis; antimicrobial-resistant genes; mobile genetic elements; virulence factors |
| Substance Nomenclature: | 0 (Virulence Factors) 0 (Anti-Bacterial Agents) |
| Entry Date(s): | Date Created: 20260723 Date Completed: 20260902 Latest Revision: 20260903 |
| Update Code: | 20260903 |
| PubMed Central ID: | PMC13532305 |
| DOI: | 10.1128/spectrum.01827-25 |
| PMID: | 42489462 |
| Βάση Δεδομένων: | MEDLINE |
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