The role of clathrin in post-Golgi secretion in plant cells.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος:	The role of clathrin in post-Golgi secretion in plant cells.
Συγγραφείς:	Adamowski M; Institute of Science and Technology Austria, Am Campus 1, 3400, Klosterneuburg, Austria.; Intercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk, Abrahama 58, 80-307, Gdańsk, Poland., Gackowski A; Intercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk, Abrahama 58, 80-307, Gdańsk, Poland., Matijević I; Institute of Science and Technology Austria, Am Campus 1, 3400, Klosterneuburg, Austria., Alotaibi SS; Department of Biotechnology, College of Science, Taif University, PO Box 11099, Taif, 21944, Saudi Arabia., Friml J; Institute of Science and Technology Austria, Am Campus 1, 3400, Klosterneuburg, Austria.
Πηγή:	The New phytologist [New Phytol] 2026 Sep; Vol. 251 (6), pp. 3401-3417. Date of Electronic Publication: 2026 Jul 20.
Τύπος έκδοσης:	Journal Article
Γλώσσα:	English
Στοιχεία περιοδικού:	Publisher: Wiley on behalf of New Phytologist Trust Country of Publication: England NLM ID: 9882884 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1469-8137 (Electronic) Linking ISSN: 0028646X NLM ISO Abbreviation: New Phytol Subsets: MEDLINE
Imprint Name(s):	Publication: Oxford : Wiley on behalf of New Phytologist Trust Original Publication: London, New York [etc.] Academic Press.
Ιατρικοί όροι (MeSH):	Arabidopsis/metabolism , Arabidopsis/cytology , Arabidopsis/genetics , Clathrin/metabolism , Plant Cells/metabolism , Golgi Apparatus/metabolism, Arabidopsis Proteins/metabolism ; Arabidopsis Proteins/genetics ; Mutation/genetics ; Cell Membrane/metabolism ; trans-Golgi Network/metabolism ; Vacuoles/metabolism ; Endocytosis ; Protein Transport ; Gene Silencing
Περίληψη:	Within the plant endomembrane system, the vesicle coat protein clathrin localizes to the plasma membrane (PM) and the trans-Golgi Network/early endosome (TGN/EE). While the role of clathrin in endocytosis at the PM is well established, its function at TGN/EE, presumably in late secretion (trafficking from the TGN/EE to the cell surface) or en route to the vacuole, is debated. Similarly debated are potential homeostatic mechanisms balancing the trafficking routes, especially endocytosis and late secretion. We address these questions in Arabidopsis thaliana using conditional silencing of CLATHRIN HEAVY CHAIN (CHC), conditional overexpression of the clathrin uncoating factor AUXILIN-LIKE1, and secretory mutants. CHC silencing interferes with trafficking of cargoes destined for the apoplast and the PM, supporting a function of clathrin in late secretion. The secretory cargoes become abnormally rerouted from the TGN/EE to the vacuole. Unlike CHC silencing, overexpression of AUXILIN-LIKE1 selectively inhibits clathrin-mediated endocytosis while secretion continues normally at early points of induction. Conversely, secretory mutants exhibit a reduced PM recruitment of clathrin, and variably, of the TPLATE endocytic component. Together, our data show a role of clathrin in secretion and suggest secretion as a fundamental trafficking process to which endocytosis is adjusted by a weak homeostatic mechanism. (© 2026 The Author(s). New Phytologist © 2026 New Phytologist Foundation.)
References:	Cell. 1985 Apr;40(4):1001-9. (PMID: 3886157) Plant Cell. 2011 May;23(5):1920-31. (PMID: 21551390) Nature. 2007 Jul 26;448(7152):493-6. (PMID: 17653191) Front Plant Sci. 2022 Aug 11;13:957995. (PMID: 36035717) Traffic. 2006 Dec;7(12):1701-23. (PMID: 17118121) Nat Protoc. 2006;1(1):98-103. (PMID: 17406218) Mol Biol Cell. 2015 May 15;26(10):1783-5. (PMID: 25972202) Proc Natl Acad Sci U S A. 2018 Apr 10;115(15):E3578-E3587. (PMID: 29581258) Annu Rev Plant Biol. 2024 Jul;75(1):521-550. (PMID: 38237062) Plant Cell. 2013 Jun;25(6):2217-35. (PMID: 23771894) J Cell Biol. 1995 Oct;131(2):297-310. (PMID: 7593160) Plant Cell. 2018 Mar;30(3):700-716. (PMID: 29511054) Plant Cell. 2017 May;29(5):1039-1052. (PMID: 28442598) Plant Cell. 2022 May 24;34(6):2150-2173. (PMID: 35218346) Plant Physiol. 2013 Aug;162(4):1897-910. (PMID: 23766365) J Cell Biol. 2002 Jan 21;156(2):271-85. (PMID: 11807092) PLoS Biol. 2016 Aug 15;14(8):e1002534. (PMID: 27526190) Development. 2018 Nov 7;145(21):. (PMID: 30404777) Plant Cell. 2011 Sep;23(9):3463-81. (PMID: 21934143) Curr Biol. 2007 Mar 20;17(6):520-7. (PMID: 17306539) Plant Physiol. 2016 Feb;170(2):627-41. (PMID: 26644504) Trends Plant Sci. 2014 Mar;19(3):134-9. (PMID: 24263003) Plant Physiol. 2017 Oct;175(2):708-720. (PMID: 28830938) Proc Natl Acad Sci U S A. 2013 Jun 18;110(25):10318-23. (PMID: 23733933) Proc Natl Acad Sci U S A. 2011 May 10;108(19):8048-53. (PMID: 21512130) Plant Cell. 2008 May;20(5):1363-80. (PMID: 18502847) Trends Cell Biol. 2015 Jul;25(7):408-16. (PMID: 25795254) Plant Cell. 2019 Dec;31(12):2833-2854. (PMID: 31628169) Plant Cell. 2014 Jul;26(7):3062-76. (PMID: 25012191) Mol Plant. 2015 Aug;8(8):1175-87. (PMID: 25795554) Elife. 2024 Feb 21;13:. (PMID: 38381485) J Exp Bot. 2017 Dec 18;69(1):21-37. (PMID: 28992209) Plant J. 2012 Jan;69(2):204-16. (PMID: 21910772) Plant Cell. 2013 Jul;25(7):2633-46. (PMID: 23832588) PLoS One. 2013 Apr 16;8(4):e60990. (PMID: 23613767) PLoS Biol. 2011 Mar;9(3):e1000604. (PMID: 21445324) J Cell Biol. 2022 Jan 3;221(1):. (PMID: 34739034) Plant Physiol. 2020 Apr;182(4):1762-1775. (PMID: 32094305) New Phytol. 2019 Dec;224(4):1479-1489. (PMID: 31230354) Cell. 1980 Aug;21(1):205-15. (PMID: 6996832) Annu Rev Biochem. 2017 Jun 20;86:659-684. (PMID: 28301744) Plant Cell. 2022 Sep 27;34(10):3961-3982. (PMID: 35766888) Cell. 2003 Nov 26;115(5):591-602. (PMID: 14651850) Mol Biol Cell. 1997 Aug;8(8):1481-99. (PMID: 9285820) Curr Opin Cell Biol. 2019 Apr;57:8-15. (PMID: 30390465) Curr Opin Plant Biol. 2013 Dec;16(6):726-33. (PMID: 24246229) Traffic. 2011 Mar;12(3):313-29. (PMID: 21134079) Elife. 2020 Jan 23;9:. (PMID: 31971511) Plant Cell. 2015 Jan;27(1):20-32. (PMID: 25604445) PLoS One. 2014 Mar 21;9(3):e93031. (PMID: 24658132) Plant Cell. 2010 Aug;22(8):2812-24. (PMID: 20729380) Elife. 2014 Apr 08;3:e02131. (PMID: 24714496) Proc Natl Acad Sci U S A. 2010 Mar 30;107(13):6094-9. (PMID: 20231465) J Cell Sci. 2024 Apr 15;137(8):. (PMID: 38506228) Proc Natl Acad Sci U S A. 2020 Oct 13;117(41):25880-25889. (PMID: 32989160) Annu Rev Cell Dev Biol. 2004;20:153-91. (PMID: 15473838) Plant Cell. 2021 Sep 24;33(9):3057-3075. (PMID: 34240193) Curr Biol. 2002 Sep 17;12(18):1623-7. (PMID: 12372257) Plant Cell. 2013 Aug;25(8):2986-97. (PMID: 23975899) Trends Plant Sci. 2002 May;7(5):193-5. (PMID: 11992820) Plant Cell Physiol. 2017 Oct 1;58(10):1801-1811. (PMID: 29016942) Cell Res. 2011 Dec;21(12):1711-22. (PMID: 21670741) EMBO J. 2002 Feb 15;21(4):602-14. (PMID: 11847108) FEBS Lett. 2022 Sep;596(17):2269-2287. (PMID: 35674447) Nat Rev Mol Cell Biol. 2018 Nov;19(11):679-696. (PMID: 30194414) Cell. 2014 Feb 13;156(4):691-704. (PMID: 24529374) Nature. 2014 Jun 5;510(7503):48-57. (PMID: 24899304) Plant Cell. 2014 Oct;26(10):4102-18. (PMID: 25351491) Nat Commun. 2021 Mar 26;12(1):1901. (PMID: 33772008) Nature. 2007 Jul 26;448(7152):488-92. (PMID: 17653190) Cell Rep. 2024 May 28;43(5):114195. (PMID: 38717900) J Microsc. 2004 May;214(Pt 2):159-73. (PMID: 15102063) J Cell Biol. 2018 Jan 2;217(1):283-298. (PMID: 29109089) J Cell Biol. 2019 May 6;218(5):1582-1601. (PMID: 30858194) New Phytol. 2009;183(2):255-272. (PMID: 19496948) Nat Commun. 2020 Feb 3;11(1):679. (PMID: 32015349) J Cell Biol. 2024 Jul 1;223(7):. (PMID: 38578286)
Grant Information:	TURSP-HC2022/02 Taif University Researchers Supporting Project; I 3630-B25 Austrian Science Fund
Contributed Indexing:	Keywords: AUXILIN‐LIKE; auxilin; clathrin; endocytosis; exocytosis; secretion; trans‐Golgi Network
Substance Nomenclature:	0 (Arabidopsis Proteins) 0 (Clathrin)
Entry Date(s):	Date Created: 20260720 Date Completed: 20260820 Latest Revision: 20260822
Update Code:	20260822
PubMed Central ID:	PMC13491243
DOI:	10.1111/nph.71454
PMID:	42477503
Βάση Δεδομένων:	MEDLINE

καταχωρήστε σχόλιο πρώτοι!