Academic Journal
Spatiotemporal dynamics of epididymal epithelial cells uncover a novel KRT5⁺ATP6V1A⁺ subpopulation of basal cell in postnatal mouse development.
| Τίτλος: | Spatiotemporal dynamics of epididymal epithelial cells uncover a novel KRT5⁺ATP6V1A⁺ subpopulation of basal cell in postnatal mouse development. |
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| Συγγραφείς: | Li H; Medical School of Nantong University, GMU-GIBH Joint School of Life Science, Guangzhou Medical University, Guangzhou, PR China., Xie T; Medical School of Nantong University, GMU-GIBH Joint School of Life Science, Guangzhou Medical University, Guangzhou, PR China., YLChan D; Assisted Reproductive Technology Unit, Department of Obstetrics and Gynecology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong., Fok EKL; School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong., Zuo L; Medical School of Nantong University, GMU-GIBH Joint School of Life Science, Guangzhou Medical University, Guangzhou, PR China., Xie G; Medical School of Nantong University, GMU-GIBH Joint School of Life Science, Guangzhou Medical University, Guangzhou, PR China., Sha Q; Medical School of Nantong University, GMU-GIBH Joint School of Life Science, Guangzhou Medical University, Guangzhou, PR China., Chen H; Medical School of Nantong University, GMU-GIBH Joint School of Life Science, Guangzhou Medical University, Guangzhou, PR China. Electronic address: chenhao@gzhmu.edu.cn. |
| Πηγή: | European journal of cell biology [Eur J Cell Biol] 2026 Sep; Vol. 105 (3), pp. 151555. Date of Electronic Publication: 2026 Jul 17. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Country of Publication: Germany NLM ID: 7906240 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1618-1298 (Electronic) Linking ISSN: 01719335 NLM ISO Abbreviation: Eur J Cell Biol Subsets: MEDLINE |
| Imprint Name(s): | Publication: Jena, Germany : Elsevier Original Publication: Stuttgart : Wissenschaftliche Verlagsgesellschaft, <1979-1997> |
| Ιατρικοί όροι (MeSH): | Epididymis*/cytology , Epididymis*/metabolism , Epididymis*/growth & development , Epithelial Cells*/metabolism , Epithelial Cells*/cytology , Vacuolar Proton-Translocating ATPases*/metabolism , Vacuolar Proton-Translocating ATPases*/genetics , Keratin-5*/metabolism , Keratin-5*/genetics, Animals ; Male ; Mice ; Rats ; Mice, Inbred C57BL |
| Περίληψη: | Epididymal epithelial cells are critical for sperm maturation. Although previous studies have determined the development of epididymal epithelial cells in rat epididymis, the postnatal developmental trajectories of distinct epithelial cell types in the mouse epididymis remain elusive. Here, we examined the developmental timeline of mouse epididymal epithelium differed from that of the rat by confocal immunostaining and single-cell RNA sequencing (scRNA-seq). In mice, principal and basal cells appeared as early as postnatal day 7 (PD7) across all segments, labeled by AQP9 and KRT5, in contrast to the later emergence in rats (e.g., basal cells at PD14 in rat cauda). ATP6V1A-positive clear cells were detected at PD28 in caput, corpus, and cauda but not in the initial segment, while narrow cells were observed in all segments beginning at PD14. These contrasted with the rat, in which clear cells appeared at PD14. Notably, scRNA-seq and immunostaining identified a previously unrecognized KRT5⁺/ATP6V1A⁺ narrow-like basal cell subpopulation, enriched in lysosomes and mainly localized to the caput and corpus. These findings revealed an earlier epithelial maturation in mice compared to rats, underscoring the importance of choosing animal models. The newly identified basal subpopulation may contribute to luminal acidification and homeostasis, offering new insights into epididymal physiology and reproductive biology. (Copyright © 2026 The Authors. Published by Elsevier GmbH.. All rights reserved.) |
| Competing Interests: | Declaration of Competing Interest The authors declare that they have no competing financial interests or personal relationships that could appear to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Basal cell subpopulation; Epididymis; Epithelial cells dynamic; Luminal acidification; Postnatal development |
| Substance Nomenclature: | EC 3.6.1.- (Vacuolar Proton-Translocating ATPases) 0 (Keratin-5) |
| Entry Date(s): | Date Created: 20260720 Date Completed: 20260812 Latest Revision: 20260812 |
| Update Code: | 20260813 |
| DOI: | 10.1016/j.ejcb.2026.151555 |
| PMID: | 42475816 |
| Βάση Δεδομένων: | MEDLINE |
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