Academic Journal
Oral delivery of recombinant Bacillus subtilis expressing mSEB reduces intestinal Staphylococcus aureus colonization.
| Τίτλος: | Oral delivery of recombinant Bacillus subtilis expressing mSEB reduces intestinal Staphylococcus aureus colonization. |
|---|---|
| Συγγραφείς: | Yang K; Clinical Laboratory, Key Laboratory of Bacterial antimicrobial Resistance and Prevention, Medical Research Institute of Maternal and Child, Affiliated Shenzhen Women and Children's Hospital (Longgang) of Shantou University Medical College (Longgang District Maternity & Child Healthcare Hospital of Shenzhen City), Shenzhen, Guangdong 518172, China., He Y; Clinical Laboratory, Key Laboratory of Bacterial antimicrobial Resistance and Prevention, Medical Research Institute of Maternal and Child, Affiliated Shenzhen Women and Children's Hospital (Longgang) of Shantou University Medical College (Longgang District Maternity & Child Healthcare Hospital of Shenzhen City), Shenzhen, Guangdong 518172, China., Zhou X; Clinical Laboratory, Key Laboratory of Bacterial antimicrobial Resistance and Prevention, Medical Research Institute of Maternal and Child, Affiliated Shenzhen Women and Children's Hospital (Longgang) of Shantou University Medical College (Longgang District Maternity & Child Healthcare Hospital of Shenzhen City), Shenzhen, Guangdong 518172, China., Ding T; Clinical Laboratory, Key Laboratory of Bacterial antimicrobial Resistance and Prevention, Medical Research Institute of Maternal and Child, Affiliated Shenzhen Women and Children's Hospital (Longgang) of Shantou University Medical College (Longgang District Maternity & Child Healthcare Hospital of Shenzhen City), Shenzhen, Guangdong 518172, China., Liu S; Clinical Laboratory, Key Laboratory of Bacterial antimicrobial Resistance and Prevention, Medical Research Institute of Maternal and Child, Affiliated Shenzhen Women and Children's Hospital (Longgang) of Shantou University Medical College (Longgang District Maternity & Child Healthcare Hospital of Shenzhen City), Shenzhen, Guangdong 518172, China., Lin S; Clinical Laboratory, Key Laboratory of Bacterial antimicrobial Resistance and Prevention, Medical Research Institute of Maternal and Child, Affiliated Shenzhen Women and Children's Hospital (Longgang) of Shantou University Medical College (Longgang District Maternity & Child Healthcare Hospital of Shenzhen City), Shenzhen, Guangdong 518172, China., Liu X; Clinical Laboratory, Key Laboratory of Bacterial antimicrobial Resistance and Prevention, Medical Research Institute of Maternal and Child, Affiliated Shenzhen Women and Children's Hospital (Longgang) of Shantou University Medical College (Longgang District Maternity & Child Healthcare Hospital of Shenzhen City), Shenzhen, Guangdong 518172, China., Zhou Z; Clinical Laboratory, Key Laboratory of Bacterial antimicrobial Resistance and Prevention, Medical Research Institute of Maternal and Child, Affiliated Shenzhen Women and Children's Hospital (Longgang) of Shantou University Medical College (Longgang District Maternity & Child Healthcare Hospital of Shenzhen City), Shenzhen, Guangdong 518172, China; Clinical Laboratory, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangdong Provincial Clinical Research Center for Laboratory medicine, Guangzhou, Guangdong 510623, China. Electronic address: zzw6248@126.com. |
| Πηγή: | Vaccine [Vaccine] 2026 Aug 13; Vol. 88, pp. 128939. Date of Electronic Publication: 2026 Jul 18. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 8406899 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2518 (Electronic) Linking ISSN: 0264410X NLM ISO Abbreviation: Vaccine Subsets: MEDLINE |
| Imprint Name(s): | Publication: Amsterdam, The Netherlands : Elsevier Science Original Publication: [Guildford, Surrey, UK] : Butterworths, [c1983- |
| Ιατρικοί όροι (MeSH): | Bacillus subtilis*/genetics , Bacillus subtilis*/immunology , Enterotoxins*/immunology , Enterotoxins*/genetics , Enterotoxins*/administration & dosage , Staphylococcus aureus*/immunology , Staphylococcal Infections*/prevention & control , Staphylococcal Infections*/immunology , Probiotics*/administration & dosage , Intestines*/microbiology, Antibodies, Bacterial/blood ; Antibodies, Bacterial/immunology ; Immunoglobulin G/blood ; Feces/microbiology ; Spores, Bacterial/immunology ; Macrophages, Peritoneal/immunology ; Animals ; Administration, Oral ; Mice ; Female ; Immunoglobulin A ; Cytokines ; Tumor Necrosis Factor-alpha |
| Περίληψη: | Background: S. aureus intestinal colonization elevates infection risk, underscoring decolonization as a key prevention target. SEB is a key target for the development of neutralizing antibodies against toxins to prevent and treat S. aureus infection. B. subtilis is used as a probiotic for human health. Objective: To investigate the efficacy of recombinant mSEB B. subtilis spores for reducing S. aureus intestinal colonization in mouse. Methods: Mice were orally immunized with recombinant mSEB spores three times a week for three weeks. After immunization, mice were challenged via oral gavage with 1 × 109 CFU of S. aureus (ATCC 14458). Fecal specific IgA and serum IgG1 and IgG2a were analyzed by ELISA. Peritoneal macrophages were isolated for qRT-PCR analysis of TNF-α, IL-6 and IL-1β mRNA expression. Colon contents samples underwent 16S rRNA sequencing for microbiota profiling. Intestinal RNA was extracted for transcriptome sequencing (RNA-seq), and differential pathways were analyzed using KEGG enrichment. Body weight and fecal viable S. aureus burden were monitored. Results: Oral mSEB spores correlated with elevated systemic and mucosal SEB-specific IgG1, IgG2a and fecal sIgA (P < 0.01). After S. aureus challenge, peritoneal macrophages from mSEB mice showed lower pro-inflammatory TNF-α, IL-6 and IL-1β transcripts, consistent with attenuated systemic inflammation. mSEB immunization also altered gut microbiota and increased the relative abundance of Barnesiella. Intestinal RNA-seq identified enriched antigen presentation and B cell receptor signaling gene sets in the mSEB group. On day 3 post-challenge, fecal S. aureus loads were 24.20 ± 3.967 × 104 CFU (Control), 8.081 ± 3.614 × 104 CFU (CotC) and 3.6 ± 1.030 × 104 CFU (mSEB), showing a pathogen-clearing phenotype linked to mSEB treatment. Conclusion: Immunization with mSEB-displaying Bacillus subtilis spores correlates with SEB-specific mucosal and systemic antibody responses, blunted systemic inflammation, modified gut microbiota, and reduced intestinal S. aureus burdens post-challenge. The causal mechanisms underlying these changes remain to be clarified. (Copyright © 2026 Elsevier Ltd. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Bacillus subtilis; Decolonization; Staphylococcal enterotoxin B; Staphylococcus aureus |
| Substance Nomenclature: | 0 (Enterotoxins) 0 (Antibodies, Bacterial) 39424-53-8 (enterotoxin B, staphylococcal) 0 (Immunoglobulin G) 0 (Immunoglobulin A) 0 (Cytokines) 0 (Tumor Necrosis Factor-alpha) |
| Entry Date(s): | Date Created: 20260719 Date Completed: 20260811 Latest Revision: 20260811 |
| Update Code: | 20260811 |
| DOI: | 10.1016/j.vaccine.2026.128939 |
| PMID: | 42472494 |
| Βάση Δεδομένων: | MEDLINE |
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