Academic Journal
Genomic surveillance of carbapenem-resistant Acinetobacter baumannii from bloodstream infections in Thailand reveals widespread dissemination of blaNDM-harboring mobile genetic elements.
| Τίτλος: | Genomic surveillance of carbapenem-resistant Acinetobacter baumannii from bloodstream infections in Thailand reveals widespread dissemination of blaNDM-harboring mobile genetic elements. |
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| Συγγραφείς: | Unahalekhaka A; National Antimicrobial Resistance Surveillance Center, Thailand (NARST), National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand.; National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand., Krobanan K; National Antimicrobial Resistance Surveillance Center, Thailand (NARST), National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand.; National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand., Kamjumphol W; National Antimicrobial Resistance Surveillance Center, Thailand (NARST), National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand.; National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand., Leethongdee P; National Antimicrobial Resistance Surveillance Center, Thailand (NARST), National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand.; National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand., Sukheephak Y; National Antimicrobial Resistance Surveillance Center, Thailand (NARST), National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand.; National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand., Boonjan P; National Antimicrobial Resistance Surveillance Center, Thailand (NARST), National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand.; National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand., Thapaan K; National Antimicrobial Resistance Surveillance Center, Thailand (NARST), National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand.; National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand., Klayut W; National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand., Sripichai O; National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand., Thadtapong N; National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand., Kurehong C; National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand., Moolwang K; National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand., Muanplueng D; National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand., Wongboot W; National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand., Okada PA; National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand., Premsuriya J; Princess Srisavangavadhana Faculty of Medicine, Chulabhorn Royal Academy, Bangkok, Thailand.; Research Center on Clinical and System Microbiology (RCSyM), Chulabhorn Royal Academy, Bangkok, Thailand. |
| Πηγή: | PloS one [PLoS One] 2026 Jul 13; Vol. 21 (7), pp. e0353488. Date of Electronic Publication: 2026 Jul 13 (Print Publication: 2026). |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: San Francisco, CA : Public Library of Science |
| Ιατρικοί όροι (MeSH): | Carbapenems*/pharmacology , Acinetobacter baumannii*/genetics , Acinetobacter baumannii*/drug effects , Acinetobacter baumannii*/isolation & purification , beta-Lactamases*/genetics , Acinetobacter Infections*/microbiology , Acinetobacter Infections*/epidemiology , Acinetobacter Infections*/drug therapy , Interspersed Repetitive Sequences*/genetics, Thailand/epidemiology ; Anti-Bacterial Agents/pharmacology ; Bacterial Proteins/genetics ; Humans ; Phylogeny ; Microbial Sensitivity Tests ; Whole Genome Sequencing |
| Περίληψη: | Carbapenem-resistant Acinetobacter baumannii (CRAB) is a major cause of hospital-acquired bloodstream infections in Thailand, with limited treatment options and a strong association with high morbidity and mortality. We performed nationwide genomic surveillance of 41 CRAB isolates from bloodstream infections collected across Thailand between 2020 and 2024 to characterize the population structure, antimicrobial resistance determinants, and the genomic context of blaNDM-harboring mobile genetic elements (MGEs). Whole-genome sequencing and core genome SNP-based phylogenetic analysis of the Thai samples revealed the dominance of international clone II (Pasteur ST2), which was widely distributed across geographic regions. Carbapenem resistance was primarily mediated by blaOXA-23, together with intrinsic blaOXA-66 and resistance-associated mutations in a penicillin-binding protein gene. In addition to OXA-type carbapenemase genes, blaNDM-5 and blaNDM-1 were detected in a subset of isolates. In particular, blaNDM-5 was located within a highly conserved MGE associated with a class 1 integron, IS91 family transposase and ISAba125 and was detected across multiple ST2 sublineages. This MGE was distributed across multiple geographic regions and phylogenetic lineages in Thailand, with evidence suggesting widespread horizontal dissemination followed by local clonal expansion. By contrast, blaNDM-1 was detected less frequently but was consistently associated with a canonical Tn125 transposon in both ST2 and ST16 isolates, highlighting its continued epidemiological relevance. Collectively, our findings demonstrate that CRAB bloodstream infections in Thailand are driven by a combination of endemic high-risk lineages and the sustained circulation of blaNDM-harboring MGEs. Overall, our results confirm the importance of continued genomic surveillance for monitoring the emergence and dissemination of blaNDM-producing CRAB and for strengthening infection prevention and antimicrobial resistance control efforts in hospital settings. (Copyright: © 2026 Unahalekhaka et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.) |
| Competing Interests: | The authors have declared that no competing interests exist. |
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| Substance Nomenclature: | 0 (Carbapenems) EC 3.5.2.6 (beta-Lactamases) 0 (Anti-Bacterial Agents) EC 3.5.2.6 (beta-lactamase NDM-1) 0 (Bacterial Proteins) |
| Entry Date(s): | Date Created: 20260713 Date Completed: 20260713 Latest Revision: 20260813 |
| Update Code: | 20260814 |
| PubMed Central ID: | PMC13362128 |
| DOI: | 10.1371/journal.pone.0353488 |
| PMID: | 42441555 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1932-6203 |
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| DOI: | 10.1371/journal.pone.0353488 |