Academic Journal
Bile Acid Deconjugation and Anti-Helicobacter pylori Activity of Limosilactobacillus reuteri DSM 34531.
| Τίτλος: | Bile Acid Deconjugation and Anti-Helicobacter pylori Activity of Limosilactobacillus reuteri DSM 34531. |
|---|---|
| Συγγραφείς: | Otaru N; Health, Nutrition & Care (HNC), DSM-Firmenich, Kaiseraugst, Switzerland., Bird JK; Bird Scientific Writing, Wassenaar, Netherlands., Soldi S; AAT-Advanced Analytical Technologies, Fiorenzuola d'Arda, Piacenza, Italy., Atanasova V; Health, Nutrition & Care (HNC), DSM-Firmenich, Kaiseraugst, Switzerland., Sagheddu V; AAT-Advanced Analytical Technologies, Fiorenzuola d'Arda, Piacenza, Italy., Ver Loren van Themaat E; Data Science, DSM-Firmenich, Delft, Netherlands., van Leeuwen J; Data Science, DSM-Firmenich, Delft, Netherlands., Steinert RE; Health, Nutrition & Care (HNC), DSM-Firmenich, Kaiseraugst, Switzerland.; Adelaide Medical School, Faculty of Health and Medical Sciences, CRE in Translating Nutritional Science to Good Health, The University of Adelaide, Adelaide, Australia. |
| Πηγή: | Molecular nutrition & food research [Mol Nutr Food Res] 2026 Jul; Vol. 70 (13), pp. e70514. |
| Τύπος έκδοσης: | Journal Article; Research Support, Non-U.S. Gov't |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 101231818 Publication Model: Print Cited Medium: Internet ISSN: 1613-4133 (Electronic) Linking ISSN: 16134125 NLM ISO Abbreviation: Mol Nutr Food Res Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Weinheim, Germany : Wiley-VCH, c2004- |
| Ιατρικοί όροι (MeSH): | Helicobacter pylori*/drug effects , Limosilactobacillus reuteri*/genetics , Limosilactobacillus reuteri*/enzymology , Limosilactobacillus reuteri*/metabolism , Bile Acids and Salts*/metabolism , Amidohydrolases*/genetics , Amidohydrolases*/metabolism, Probiotics/pharmacology ; Urease/metabolism ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Humans |
| Περίληψη: | Limosilactobacillus reuteri DSM 34531 has been attributed with probiotic characteristics, including a reduction of symptoms of acute diarrhea and atopic dermatitis. Yet, other probiotic features typical for some Lm. reuteri strains such as bile salt hydrolase (BSH) and anti- Helicobacter pylori activity have hitherto not been documented. Whole genome sequencing of Lm. reuteri DSM 34531 was performed to identify BSH genes, followed by BSH classification into previously described BSH clades. Suspended cell pellets and supernatants were tested for BSH activity via a quantitative colorimetric method. Auto- and co-aggregation, and urease activity of single and mixed Lm. reuteri DSM 34531 and H. pylori DSM 21031T cultures were evaluated using optical density- and microscopy-based approaches. Genome analysis of Lm. reuteri DSM 34531 revealed a single BSH-encoding gene, with its protein sequence clustering closest to the BSH-T3 phylotype. Corresponding in vitro BSH activity was confirmed. Lm. reuteri DSM 34531 co-aggregated with H. pylori DSM 21031T and reduced its urease activity. Conclusively, our data suggest that Lm. reuteri DSM 34531 possesses an active BSH, suggesting potential relevance to cholesterol metabolism. We also demonstrate in vitro co-aggregation with H. pylori while reducing its urease activity indicating potential anti-H. pylori activity under in vitro conditions. (© 2026 DSM‐Firmenich. Molecular Nutrition & Food Research published by Wiley‐VCH GmbH.) |
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| Grant Information: | DSM-Firmenich |
| Contributed Indexing: | Keywords: Lactobacillaceae; bile salt hydrolase; co‐aggregation; probiotic; urease |
| Molecular Sequence: | GENBANK PV387302 |
| Substance Nomenclature: | 0 (Bile Acids and Salts) EC 3.5.1.24 (choloylglycine hydrolase) EC 3.5.- (Amidohydrolases) EC 3.5.1.5 (Urease) 0 (Bacterial Proteins) |
| Entry Date(s): | Date Created: 20260710 Date Completed: 20260710 Latest Revision: 20260728 |
| Update Code: | 20260729 |
| PubMed Central ID: | PMC13352470 |
| DOI: | 10.1002/mnfr.70514 |
| PMID: | 42429191 |
| Βάση Δεδομένων: | MEDLINE |
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