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Bile Acid Deconjugation and Anti-Helicobacter pylori Activity of Limosilactobacillus reuteri DSM 34531.

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Title: Bile Acid Deconjugation and Anti-Helicobacter pylori Activity of Limosilactobacillus reuteri DSM 34531.
Authors: Otaru N; Health, Nutrition & Care (HNC), DSM-Firmenich, Kaiseraugst, Switzerland., Bird JK; Bird Scientific Writing, Wassenaar, Netherlands., Soldi S; AAT-Advanced Analytical Technologies, Fiorenzuola d'Arda, Piacenza, Italy., Atanasova V; Health, Nutrition & Care (HNC), DSM-Firmenich, Kaiseraugst, Switzerland., Sagheddu V; AAT-Advanced Analytical Technologies, Fiorenzuola d'Arda, Piacenza, Italy., Ver Loren van Themaat E; Data Science, DSM-Firmenich, Delft, Netherlands., van Leeuwen J; Data Science, DSM-Firmenich, Delft, Netherlands., Steinert RE; Health, Nutrition & Care (HNC), DSM-Firmenich, Kaiseraugst, Switzerland.; Adelaide Medical School, Faculty of Health and Medical Sciences, CRE in Translating Nutritional Science to Good Health, The University of Adelaide, Adelaide, Australia.
Source: Molecular nutrition & food research [Mol Nutr Food Res] 2026 Jul; Vol. 70 (13), pp. e70514.
Publication Type: Journal Article; Research Support, Non-U.S. Gov't
Language: English
Journal Info: Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 101231818 Publication Model: Print Cited Medium: Internet ISSN: 1613-4133 (Electronic) Linking ISSN: 16134125 NLM ISO Abbreviation: Mol Nutr Food Res Subsets: MEDLINE
Imprint Name(s): Original Publication: Weinheim, Germany : Wiley-VCH, c2004-
MeSH Terms: Helicobacter pylori*/drug effects , Limosilactobacillus reuteri*/genetics , Limosilactobacillus reuteri*/enzymology , Limosilactobacillus reuteri*/metabolism , Bile Acids and Salts*/metabolism , Amidohydrolases*/genetics , Amidohydrolases*/metabolism, Probiotics/pharmacology ; Urease/metabolism ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Humans
Abstract: Limosilactobacillus reuteri DSM 34531 has been attributed with probiotic characteristics, including a reduction of symptoms of acute diarrhea and atopic dermatitis. Yet, other probiotic features typical for some Lm. reuteri strains such as bile salt hydrolase (BSH) and anti- Helicobacter pylori activity have hitherto not been documented. Whole genome sequencing of Lm. reuteri DSM 34531 was performed to identify BSH genes, followed by BSH classification into previously described BSH clades. Suspended cell pellets and supernatants were tested for BSH activity via a quantitative colorimetric method. Auto- and co-aggregation, and urease activity of single and mixed Lm. reuteri DSM 34531 and H. pylori DSM 21031T cultures were evaluated using optical density- and microscopy-based approaches. Genome analysis of Lm. reuteri DSM 34531 revealed a single BSH-encoding gene, with its protein sequence clustering closest to the BSH-T3 phylotype. Corresponding in vitro BSH activity was confirmed. Lm. reuteri DSM 34531 co-aggregated with H. pylori DSM 21031T and reduced its urease activity. Conclusively, our data suggest that Lm. reuteri DSM 34531 possesses an active BSH, suggesting potential relevance to cholesterol metabolism. We also demonstrate in vitro co-aggregation with H. pylori while reducing its urease activity indicating potential anti-H. pylori activity under in vitro conditions.
(© 2026 DSM‐Firmenich. Molecular Nutrition & Food Research published by Wiley‐VCH GmbH.)
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C. Martoni, J. Bhathena, A. M. Urbanska, and S. Prakash, “Microencapsulated Bile Salt Hydrolase Producing Lactobacillus Reuteri for Oral Targeted Delivery in the Gastrointestinal Tract,” Applied Microbiology and Biotechnology 81 (2008): 225–233, https://doi.org/10.1007/s00253‐008‐1642‐8.
M. Begley, C. Hill, and C. G. M. Gahan, “Bile Salt Hydrolase Activity in Probiotics,” Applied and Environmental Microbiology 72 (2006): 1729–1738, https://doi.org/10.1128/AEM.72.3.1729‐1738.2006.
M. H. Foley, S. O. Flaherty, R. Barrangou, and C. M. Theriot, “Bile Salt Hydrolases: Gatekeepers of Bile Acid Metabolism and Host‐Microbiome Crosstalk in the Gastrointestinal Tract,” PLOS Pathogens 15 (2019): 1007581, https://doi.org/10.1371/journal.ppat.1007581.
G. Agolino, A. Pino, A. Vaccalluzzo, et al., “Bile Salt Hydrolase: The Complexity Behind its Mechanism in Relation to Lowering‐Cholesterol Lactobacilli Probiotics,” Journal of Functional Foods 120 (2024): 106357, https://doi.org/10.1016/j.jff.2024.106357.
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Grant Information: DSM-Firmenich
Contributed Indexing: Keywords: Lactobacillaceae; bile salt hydrolase; co‐aggregation; probiotic; urease
Molecular Sequence: GENBANK PV387302
Substance Nomenclature: 0 (Bile Acids and Salts)
EC 3.5.1.24 (choloylglycine hydrolase)
EC 3.5.- (Amidohydrolases)
EC 3.5.1.5 (Urease)
0 (Bacterial Proteins)
Entry Date(s): Date Created: 20260710 Date Completed: 20260710 Latest Revision: 20260728
Update Code: 20260729
PubMed Central ID: PMC13352470
DOI: 10.1002/mnfr.70514
PMID: 42429191
Database: MEDLINE
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  Data: Bile Acid Deconjugation and Anti-Helicobacter pylori Activity of Limosilactobacillus reuteri DSM 34531.
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  Data: <searchLink fieldCode="MM" term="%22Helicobacter+pylori%22">Helicobacter pylori*</searchLink>/<searchLink fieldCode="MM" term="%22Helicobacter+pylori+drug+effects%22">drug effects</searchLink> <br /><searchLink fieldCode="MM" term="%22Limosilactobacillus+reuteri%22">Limosilactobacillus reuteri*</searchLink>/<searchLink fieldCode="MM" term="%22Limosilactobacillus+reuteri+genetics%22">genetics</searchLink> <br /><searchLink fieldCode="MM" term="%22Limosilactobacillus+reuteri%22">Limosilactobacillus reuteri*</searchLink>/<searchLink fieldCode="MM" term="%22Limosilactobacillus+reuteri+enzymology%22">enzymology</searchLink> <br /><searchLink fieldCode="MM" term="%22Limosilactobacillus+reuteri%22">Limosilactobacillus reuteri*</searchLink>/<searchLink fieldCode="MM" term="%22Limosilactobacillus+reuteri+metabolism%22">metabolism</searchLink> <br /><searchLink fieldCode="MM" term="%22Bile+Acids+and+Salts%22">Bile Acids and Salts*</searchLink>/<searchLink fieldCode="MM" term="%22Bile+Acids+and+Salts+metabolism%22">metabolism</searchLink> <br /><searchLink fieldCode="MM" term="%22Amidohydrolases%22">Amidohydrolases*</searchLink>/<searchLink fieldCode="MM" term="%22Amidohydrolases+genetics%22">genetics</searchLink> <br /><searchLink fieldCode="MM" term="%22Amidohydrolases%22">Amidohydrolases*</searchLink>/<searchLink fieldCode="MM" term="%22Amidohydrolases+metabolism%22">metabolism</searchLink><br /><searchLink fieldCode="MH" term="%22Probiotics%22">Probiotics</searchLink>/<searchLink fieldCode="MH" term="%22Probiotics+pharmacology%22">pharmacology</searchLink> ; <searchLink fieldCode="MH" term="%22Urease%22">Urease</searchLink>/<searchLink fieldCode="MH" term="%22Urease+metabolism%22">metabolism</searchLink> ; <searchLink fieldCode="MH" term="%22Bacterial+Proteins%22">Bacterial Proteins</searchLink>/<searchLink fieldCode="MH" term="%22Bacterial+Proteins+genetics%22">genetics</searchLink> ; <searchLink fieldCode="MH" term="%22Bacterial+Proteins%22">Bacterial Proteins</searchLink>/<searchLink fieldCode="MH" term="%22Bacterial+Proteins+metabolism%22">metabolism</searchLink> ; <searchLink fieldCode="MH" term="%22Humans%22">Humans</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Limosilactobacillus reuteri DSM 34531 has been attributed with probiotic characteristics, including a reduction of symptoms of acute diarrhea and atopic dermatitis. Yet, other probiotic features typical for some Lm. reuteri strains such as bile salt hydrolase (BSH) and anti- Helicobacter pylori activity have hitherto not been documented. Whole genome sequencing of Lm. reuteri DSM 34531 was performed to identify BSH genes, followed by BSH classification into previously described BSH clades. Suspended cell pellets and supernatants were tested for BSH activity via a quantitative colorimetric method. Auto- and co-aggregation, and urease activity of single and mixed Lm. reuteri DSM 34531 and H. pylori DSM 21031T cultures were evaluated using optical density- and microscopy-based approaches. Genome analysis of Lm. reuteri DSM 34531 revealed a single BSH-encoding gene, with its protein sequence clustering closest to the BSH-T3 phylotype. Corresponding in vitro BSH activity was confirmed. Lm. reuteri DSM 34531 co-aggregated with H. pylori DSM 21031T and reduced its urease activity. Conclusively, our data suggest that Lm. reuteri DSM 34531 possesses an active BSH, suggesting potential relevance to cholesterol metabolism. We also demonstrate in vitro co-aggregation with H. pylori while reducing its urease activity indicating potential anti-H. pylori activity under in vitro conditions.<br /> (© 2026 DSM‐Firmenich. Molecular Nutrition & Food Research published by Wiley‐VCH GmbH.)
– Name: Ref
  Label: References
  Group: RefInfo
  Data: Z. Yu, J. Chen, Y. Liu, et al., “The Role of Potential Probiotic Strains Lactobacillus Reuteri in Various Intestinal Diseases: New Roles for an Old Player,” Frontiers in Microbiology 14 (2023): 1095555, https://doi.org/10.3389/fmicb.2023.1095555.<br />C. Hill, F. Guarner, G. Reid, et al., “The International Scientific Association for Probiotics and Prebiotics Consensus Statement on the Scope and Appropriate use of the Term Probiotic,” Nature Reviews Gastroenterology & Hepatology 11 (2014): 506–514, https://doi.org/10.1038/nrgastro.2014.66.<br />M. L. Jones, C. Tomaro‐Duchesneau, C. J. Martoni, and S. Prakash, “Cholesterol Lowering With Bile Salt Hydrolase‐Active Probiotic Bacteria, Mechanism of Action, Clinical Evidence, and Future Direction for Heart Health Applications,” Expert Opinion on Biological Therapy 13 (2013): 631–642.<br />C. Martoni, J. Bhathena, A. M. Urbanska, and S. Prakash, “Microencapsulated Bile Salt Hydrolase Producing Lactobacillus Reuteri for Oral Targeted Delivery in the Gastrointestinal Tract,” Applied Microbiology and Biotechnology 81 (2008): 225–233, https://doi.org/10.1007/s00253‐008‐1642‐8.<br />M. Begley, C. Hill, and C. G. M. Gahan, “Bile Salt Hydrolase Activity in Probiotics,” Applied and Environmental Microbiology 72 (2006): 1729–1738, https://doi.org/10.1128/AEM.72.3.1729‐1738.2006.<br />M. H. Foley, S. O. Flaherty, R. Barrangou, and C. M. Theriot, “Bile Salt Hydrolases: Gatekeepers of Bile Acid Metabolism and Host‐Microbiome Crosstalk in the Gastrointestinal Tract,” PLOS Pathogens 15 (2019): 1007581, https://doi.org/10.1371/journal.ppat.1007581.<br />G. Agolino, A. Pino, A. Vaccalluzzo, et al., “Bile Salt Hydrolase: The Complexity Behind its Mechanism in Relation to Lowering‐Cholesterol Lactobacilli Probiotics,” Journal of Functional Foods 120 (2024): 106357, https://doi.org/10.1016/j.jff.2024.106357.<br />M. L. Jones, C. J. Martoni, M. Parent, and S. Prakash, “Cholesterol‐Lowering Efficacy of a Microencapsulated Bile Salt Hydrolase‐Active Lactobacillus Reuteri NCIMB 30242 Yoghurt Formulation in Hypercholesterolaemic Adults,” British Journal of Nutrition 107 (2012): 1505–1513, https://doi.org/10.1017/S0007114511004703.<br />S. O'Flaherty, A. Briner Crawley, C. M. Theriot, and R. Barrangou, “The Lactobacillus Bile Salt Hydrolase Repertoire Reveals Niche‐Specific Adaptation,” mSphere 3 (2018): 333.<br />M. Keikha and M. Karbalaei, “Probiotics as the Live Microscopic Fighters Against Helicobacter Pylori Gastric Infections,” BMC Gastroenterology 21 (2021): 388, https://doi.org/10.1186/s12876‐021‐01977‐1.<br />C. Holz, A. Busjahn, H. Mehling, et al., “Significant Reduction in Helicobacter Pylori Load in Humans With Non‐Viable Lactobacillus Reuteri DSM17648: A Pilot Study,” Probiotics and Antimicrobial Proteins 7 (2015): 91–100, https://doi.org/10.1007/s12602‐014‐9181‐3.<br />M. Liu, H. Gao, J. 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              Text: 2026 Jul
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