Academic Journal
Recombination, mobile genetic elements, and genetic transfer contribute to the adaptation of Streptococcus uberis causing mastitis.
| Τίτλος: | Recombination, mobile genetic elements, and genetic transfer contribute to the adaptation of Streptococcus uberis causing mastitis. |
|---|---|
| Συγγραφείς: | Srithanasuwan A; Infectious Disease Epidemiology, Wageningen University, Droevendaalsesteeg 1, 6708PB, Wageningen, The Netherlands.; School of Veterinary Medicine, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, 50100, Thailand., Zou Y; Infectious Disease Epidemiology, Wageningen University, Droevendaalsesteeg 1, 6708PB, Wageningen, The Netherlands., Zadoks RN; Sydney School of Veterinary Science, Faculty of Science, University of Sydney, Camden, NSW, 2570, Australia., Suriyasathaporn W; School of Veterinary Medicine, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, 50100, Thailand. suriyasathaporn.witaya.y3@f.mail.nagoya-u.ac.jp.; Research Center of Producing and Development of Products and Innovations for Animal Health and Production, Chiang Mai University, Chiang Mai, 50100, Thailand. suriyasathaporn.witaya.y3@f.mail.nagoya-u.ac.jp.; Cambodia Campus, Asian Satellite Campuses Institute, Nagoya University, Nagoya, 464-8601, Japan. suriyasathaporn.witaya.y3@f.mail.nagoya-u.ac.jp., Schukken YH; Infectious Disease Epidemiology, Wageningen University, Droevendaalsesteeg 1, 6708PB, Wageningen, The Netherlands. ynte.schukken@wur.nl.; GD Animal Health, PO Box 9, 7400 AA, Deventer, The Netherlands. ynte.schukken@wur.nl.; Department of Population Health Sciences, Utrecht University, 3584 CL, Utrecht, The Netherlands. ynte.schukken@wur.nl. |
| Πηγή: | Veterinary research [Vet Res] 2026 Jul 07; Vol. 57 (1). Date of Electronic Publication: 2026 Jul 07. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: BioMed Central Country of Publication: England NLM ID: 9309551 Publication Model: Electronic Cited Medium: Internet ISSN: 1297-9716 (Electronic) Linking ISSN: 09284249 NLM ISO Abbreviation: Vet Res Subsets: MEDLINE |
| Imprint Name(s): | Publication: 2011- : London : BioMed Central Original Publication: Paris : Editions Scientifiques Elsevier ; INRA, c1993- |
| Ιατρικοί όροι (MeSH): | Streptococcus*/genetics , Streptococcus*/physiology , Mastitis, Bovine*/microbiology , Streptococcal Infections*/veterinary , Streptococcal Infections*/microbiology , Interspersed Repetitive Sequences* , Recombination, Genetic* , Gene Transfer, Horizontal*, Adaptation, Physiological/genetics ; Animals ; Cattle ; Genome, Bacterial ; Female ; Thailand |
| Περίληψη: | Streptococcus uberis is a major cause of bovine mastitis. However, the genomic mechanisms that facilitate adaptation of the pathogen within different host-associated environment or selection pressures remain poorly understood. This study analyzed whole-genome sequence data from three Thai dairy herds to investigate the contributions of recombination and mobile genetic elements (MGEs) to S. uberis evolution and adaptation. Among the 138 S. uberis genomes, 42 core genome sequence types (cgSTs) were identified, along with frequent detection of MGEs such as plasmid-associated genes (81.1% of isolates), prophages (67.4% of isolates), and insertion sequences (26.1% of isolates). The isolates from farm A exhibited the longest recombined fragment size, but with extremely low recombination frequency and recombination-to-mutation ratio. By contrast, the isolates from farm B, which had the highest prevalence of antimicrobial resistance (AMR) gene, showed a high recombination-to-mutation ratio (R/θ = 4.42) and more frequently contained MGEs associated with AMR genes. Finally, isolates from farm C shared a single core genome and AMR profile but harbored diverse prophages. Several prophages shared high sequence similarity (>99%) with phages infecting other bacterial genera, suggesting that ecological overlap between bacterial species may facilitate cross-genus genetic exchange, highlighting the influence of microbial ecology on the evolution of S. uberis. Collectively, our results illustrate the variety of mechanisms and genetic elements that contribute to the adaptive evolution of S. uberis in dairy farming environments. (© 2026. The Author(s).) |
| Competing Interests: | Declarations. Ethics approval and consent to participate: All S. uberis genome sequences used in this study were derived from a previous study approved by the Chiang Mai University Animal Care and Use Committee (Ref. no. S26/2562). No new animal sampling was conducted. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. |
| References: | Genome Res. 2018 Sep;28(9):1395-1404. (PMID: 30049790) Ir Vet J. 2005 Jun 01;58(6):333-7. (PMID: 21851671) Sci Rep. 2021 Nov 24;11(1):22859. (PMID: 34819576) Anim Reprod Sci. 2017 Oct;185:109-117. (PMID: 28869109) J Med Microbiol. 2024 Oct;73(10):. (PMID: 39412238) Mob Genet Elements. 2011 May;1(1):55-65. (PMID: 22016845) BMC Vet Res. 2022 Jul 7;18(1):264. (PMID: 35799261) Sci Rep. 2021 Feb 4;11(1):3046. (PMID: 33542314) Antimicrob Agents Chemother. 2015 Nov 23;60(2):1164-6. (PMID: 26596938) Wellcome Open Res. 2018 Sep 24;3:124. (PMID: 30345391) Nucleic Acids Res. 2006 Jan 1;34(Database issue):D32-6. (PMID: 16381877) Microb Genom. 2025 Jul;11(7):. (PMID: 40709905) Nat Commun. 2024 Apr 24;15(1):3477. (PMID: 38658529) Front Microbiol. 2022 Jan 06;12:773284. (PMID: 35069478) Animals (Basel). 2022 Nov 25;12(23):. (PMID: 36496808) Viruses. 2016 Mar 01;8(3):66. (PMID: 26938550) J Dairy Sci. 2025 Sep;108(9):10173-10185. (PMID: 40645489) Asian-Australas J Anim Sci. 2012 Sep;25(9):1322-8. (PMID: 25049697) BMC Genomics. 2013 Dec 27;14:920. (PMID: 24369756) mBio. 2025 Mar 12;16(3):e0285224. (PMID: 39976435) PLoS One. 2025 Aug 18;20(8):e0330304. (PMID: 40824964) J Dairy Sci. 2013 Feb;96(2):962-70. (PMID: 23200465) J Dairy Sci. 2020 Apr;103(4):3565-3576. (PMID: 32037160) BMC Vet Res. 2021 Oct 7;17(1):321. (PMID: 34620161) PLoS One. 2007 Mar 21;2(3):e315. (PMID: 17375201) Sci Rep. 2024 Feb 2;14(1):2745. (PMID: 38302507) FEMS Microbes. 2024 Sep 09;5:xtae027. (PMID: 39391373) Microb Genom. 2016 Apr 29;2(4):e000056. (PMID: 28348851) Mol Biol Evol. 2006 Feb;23(2):254-67. (PMID: 16221896) Int J Food Microbiol. 2022 Oct 2;378:109821. (PMID: 35816956) Animals (Basel). 2022 Sep 07;12(18):. (PMID: 36139187) J Antimicrob Chemother. 2021 Jan 1;76(1):101-109. (PMID: 33009809) Microb Genom. 2024 Jul;10(7):. (PMID: 39017043) J Dairy Sci. 1996 Jan;79(1):62-70. (PMID: 8675783) PLoS One. 2025 Jun 17;20(6):e0324920. (PMID: 40526704) Virology. 2016 Sep;496:42-50. (PMID: 27254594) J Dairy Sci. 2022 Feb;105(2):1504-1518. (PMID: 34955276) Front Microbiol. 2020 Aug 31;11:1993. (PMID: 32983017) Vet Microbiol. 2025 Jan;300:110319. (PMID: 39637769) Antibiotics (Basel). 2024 Apr 25;13(5):. (PMID: 38786118) Nucleic Acids Res. 2023 Jul 5;51(W1):W443-W450. (PMID: 37194694) Antibiotics (Basel). 2025 Mar 12;14(3):. (PMID: 40149107) Int J Mol Sci. 2024 Jul 26;25(15):. (PMID: 39125755) Microb Genom. 2021 Sep;7(9):. (PMID: 34486971) Nucleic Acids Res. 2019 Jul 2;47(W1):W74-W80. (PMID: 31114893) Clin Microbiol Rev. 2018 Aug 1;31(4):. (PMID: 30068738) Microb Genom. 2024 Oct;10(10):. (PMID: 39418095) Antibiotics (Basel). 2026 Jan 01;15(1):. (PMID: 41594068) BMC Microbiol. 2019 Jan 28;19(1):24. (PMID: 30691408) Appl Environ Microbiol. 2010 Dec;76(24):7957-65. (PMID: 20952646) PLoS Comput Biol. 2015 Feb 12;11(2):e1004041. (PMID: 25675341) Front Genet. 2015 Jun 15;6:214. (PMID: 26124774) Chemosphere. 2024 Dec;369:143808. (PMID: 39608649) J Dairy Sci. 2016 Dec;99(12):9864-9874. (PMID: 27743665) Res Vet Sci. 2023 Nov;164:105032. (PMID: 37844492) Bioinformatics. 2024 Jun 3;40(6):. (PMID: 38870520) |
| Contributed Indexing: | Keywords: Streptococcus uberis; antimicrobial resistance; mobile genetic elements; prophage; recombinant event |
| SCR Organism: | Streptococcus uberis |
| Entry Date(s): | Date Created: 20260708 Date Completed: 20260708 Latest Revision: 20260813 |
| Update Code: | 20260814 |
| PubMed Central ID: | PMC13343983 |
| DOI: | 10.1186/s13567-026-01795-x |
| PMID: | 42415201 |
| Βάση Δεδομένων: | MEDLINE |
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