Academic Journal
Anorectal fibrosis in 3 murine models of chronic intestinal fibrosis: a comparative analysis.
| Τίτλος: | Anorectal fibrosis in 3 murine models of chronic intestinal fibrosis: a comparative analysis. |
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| Συγγραφείς: | Ge Y; Department of Anorectal Surgery, Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, Jiangsu, 210006, China., Li M; Department of Anorectal Surgery, Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, Jiangsu, 210006, China., Li T; Department of Anorectal Surgery, Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, Jiangsu, 210006, China., Guo D; Department of Anorectal Surgery, Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, Jiangsu, 210006, China., Yu M; Department of Pathology, Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, Jiangsu, 210006, China., Yue J; Department of Radiology, Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, Jiangsu, 210006, China., Liu X; Department of TCM Proctology, Hohhot Traditional Chinese Medicine and Mongolian Medicine Hospital, Hohhot, Inner Mongolia, 010030, China., Liu Y; Proctology Department, The Affiliated Jiangsu Shengze Hospital of Nanjing Medical University, Suzhou, Jiangsu, 215228, China., Leng Q; Department of Anorectal Surgery, Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, Jiangsu, 210006, China., Ding K; Department of Anorectal Surgery, Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, Jiangsu, 210006, China. |
| Πηγή: | Inflammatory bowel diseases [Inflamm Bowel Dis] 2026 Aug 01; Vol. 32 (8), pp. 1547-1560. |
| Τύπος έκδοσης: | Journal Article; Comparative Study |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Oxford University Press Country of Publication: England NLM ID: 9508162 Publication Model: Print Cited Medium: Internet ISSN: 1536-4844 (Electronic) Linking ISSN: 10780998 NLM ISO Abbreviation: Inflamm Bowel Dis Subsets: MEDLINE |
| Imprint Name(s): | Publication: 2018- : [Oxford] : Oxford University Press Original Publication: New York, NY : Raven Press, c1995- |
| Ιατρικοί όροι (MeSH): | Fibrosis*/pathology , Fibrosis*/chemically induced , Colitis*/chemically induced , Colitis*/pathology , Colitis*/complications , Anal Canal*/pathology , Rectum*/pathology , Disease Models, Animal*, Trinitrobenzenesulfonic Acid/toxicity ; Dextran Sulfate/toxicity ; Animals ; Male ; Mice ; Mice, Inbred C57BL ; Chronic Disease ; Benzenesulfonates |
| Περίληψη: | Background: Anorectal fibrosis is a poorly understood condition of Crohn's disease (CD)-associated perianal complications, characterized by transmural fibrotic remodeling that may contribute to luminal narrowing. Despite well-established animal models for intestinal fibrosis, the anorectal region remains largely unexplored. This study aimed to compare anorectal fibrotic remodeling across 3 commonly used chronic intestinal fibrosis protocols. Methods: Male C57BL/6 mice were assigned to control and 3 chronic colitis groups: dextran sulfate sodium (DSS; cyclic oral administration), 2,4,6-trinitrobenzenesulfonic acid (TNBS), and dinitrobenzene sulfonic acid (DNBS; weekly intrarectal administration). Anorectal fibrosis was assessed using pelvic MRI, histopathological analysis (H&E, Masson's trichrome, Sirius red staining), polarized light microscopy for collagen typing, and immunohistochemistry for fibrosis markers (α-SMA, TGF-β1, collagen I) and inflammatory cells (F4/80, MPO, CD3). Molecular analysis included quantitative RT-PCR and hydroxyproline assay. Results: All 3 models developed comparable colonic inflammation and fibrosis. However, at the anorectal transition zone, DSS-treated mice exhibited significantly greater wall thickness on MRI, more severe collagen deposition, elevated type I/III collagen ratio indicative of mature fibrosis, and enhanced myofibroblast activation with TGF-β1 upregulation, muscularis propria thickening, and reduced luminal area on histologic cross-sections. In the anal squamous epithelium, DSS mice demonstrated significantly increased subepithelial fibrosis, epithelial hyperplasia, and diffuse inflammatory cell infiltration throughout both epithelial and subepithelial layers. Conclusions: Under these standard protocols, the chronic cyclic DSS model produced the most prominent anorectal fibrotic remodeling, while TNBS and DNBS showed less severe anorectal changes despite comparable colonic fibrosis. These protocol-dependent differences may inform preclinical investigation of anorectal fibrosis and evaluation of anti-fibrotic therapeutic strategies. (© The Author(s) 2026. Published by Oxford University Press on behalf of Crohn’s & Colitis Foundation. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.) |
| Grant Information: | 82200579 National Natural Science Foundation of China; JD2023SZX10 Project of National Clinical Research Base of Traditional Chinese Medicine in Jiangsu Province; JD2023SZX10 National Clinical Research Base of Traditional Chinese Medicine in Jiangsu Province, China |
| Contributed Indexing: | Keywords: Crohn’s disease; animal model; anorectal fibrosis |
| Substance Nomenclature: | 8T3HQG2ZC4 (Trinitrobenzenesulfonic Acid) 9042-14-2 (Dextran Sulfate) 12379-41-8 (dinitrobenzenesulfonic acid) 0 (Benzenesulfonates) |
| Entry Date(s): | Date Created: 20260707 Date Completed: 20260729 Latest Revision: 20260729 |
| Update Code: | 20260730 |
| DOI: | 10.1093/ibd/izag138 |
| PMID: | 42411386 |
| Βάση Δεδομένων: | MEDLINE |
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