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Preliminary strain-specific and non-additive effects of single versus mixed DSE inoculation on rhizosphere microbiome and nutrient cycling relative to plant biomass in Chinese yam.

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Title: Preliminary strain-specific and non-additive effects of single versus mixed DSE inoculation on rhizosphere microbiome and nutrient cycling relative to plant biomass in Chinese yam.
Authors: Su Z; College of Traditional Chinese Medicine, Hebei University, Baoding, Hebei 071000, China., Li M; College of Traditional Chinese Medicine, Hebei University, Baoding, Hebei 071000, China., Zuo Y; College of Traditional Chinese Medicine, Hebei University, Baoding, Hebei 071000, China.; Hebei Administration of Traditional Chinese Medicine Collaborative Innovation Key Research Laboratory of Chinese Medicinal Resource Industrialization, Baoding, Hebei 071000, China., Guo J; College of Traditional Chinese Medicine, Hebei University, Baoding, Hebei 071000, China., Wei Y; College of Traditional Chinese Medicine, Hebei University, Baoding, Hebei 071000, China., Fan S; College of Traditional Chinese Medicine, Hebei University, Baoding, Hebei 071000, China.; Hebei Administration of Traditional Chinese Medicine Collaborative Innovation Key Research Laboratory of Chinese Medicinal Resource Industrialization, Baoding, Hebei 071000, China., Wang Y; School of Basic Medical Sciences, Hebei University, Baoding, Hebei 071000, China.; Hebei Administration of Traditional Chinese Medicine Collaborative Innovation Key Research Laboratory of Chinese Medicinal Resource Industrialization, Baoding, Hebei 071000, China.
Source: Journal of applied microbiology [J Appl Microbiol] 2026 Aug 04; Vol. 137 (8).
Publication Type: Journal Article
Language: English
Journal Info: Publisher: Oxford University Press Country of Publication: England NLM ID: 9706280 Publication Model: Print Cited Medium: Internet ISSN: 1365-2672 (Electronic) Linking ISSN: 13645072 NLM ISO Abbreviation: J Appl Microbiol Subsets: MEDLINE
Imprint Name(s): Publication: 2022- : Oxford : Oxford University Press
Original Publication: Oxford : Published for the Society for Applied Bacteriology by Blackwell Science, c1997-
MeSH Terms: Dioscorea*/microbiology , Dioscorea*/growth & development , Dioscorea*/metabolism , Endophytes*/physiology , Endophytes*/isolation & purification , Endophytes*/genetics , Endophytes*/classification , Rhizosphere* , Soil Microbiology* , Microbiota*, Plant Roots/microbiology ; Plant Roots/growth & development ; Bacteria/classification ; Bacteria/isolation & purification ; Bacteria/genetics ; Bacteria/metabolism ; Fungi/classification ; Fungi/isolation & purification ; Fungi/genetics ; Fungi/metabolism ; Soil/chemistry ; Biomass ; China
Abstract: Aims: To mitigate Chinese yam continuous cropping obstacles, this study assessed single and mixed dark septate endophyte (DSE) inoculation effects on yam growth, soil characteristics and rhizosphere microbial communities, offering references for optimizing rhizosphere microenvironment of continuously cropped medicinal plant soils.
Methods and Results: 9 DSE strains were isolated from yam roots for single/mixed seedling pot inoculation; We found that growth-promoting effects differed by strain. Acrocalymma and Setophoma terrestris were the most commonly isolated DSE species, but strains with lower isolation rates Paraphoma ledniceana, Amesia atrobrunnea, and Zopfiella pilifera exhibited the most pronounced positive effects on yam root biomass. Increasing the number of co-inoculated strains did not proportionally enhance yam growth but progressively restructured soil microbial communities. However, mixed DSE inoculation as a whole showed no obvious growth promotion compared with the control. Fungal co-occurrence networks exhibited higher modularity and clustering, while bacterial communities had greater connectivity, especially under P. ledniceana and Fusarium sp. inoculation. Biosynthesis was the most abundant predicted function of rhizosphere microbes, with bacteria predicted to be enriched in detoxification and fungi in degradation-related pathways. Two strains of Chaetomiaceae and Periconia epilithographicola increased soil pH, organic carbon and available phosphorus, while available nitrogen decreased after DSE inoculation. Importantly, P. epilithographicola inoculation enhanced mycorrhizal fungi and mitigated microbial imbalance.
Conclusions: Single and mixed DSE inoculations regulate rhizosphere microbial assembly and nutrient cycling via species-specific effects. Screening beneficial DSE consortia and characterizing their influence on rhizosphere networks provides preliminary theoretical insights and and candidate strains that may relieve yam replant obstacles in future applications.
(© The Author(s) 2026. Published by Oxford University Press on behalf of Applied Microbiology International. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site-for further information please contact journals.permissions@oup.com.)
Grant Information: C2024201069 Natural Science Foundation of Hebei Province; QN2025140 Natural Science Foundation of Hebei Provincial Universities; 20240031 Hebei University; 20240053 Hebei University; CXZZSS2025011 Innovation Fund Project of Hebei Province
Contributed Indexing: Keywords: Chinese Yam; co-occurrence networks; dark septate endophytes; mixed microbial inoculation; rhizosphere interactions
Substance Nomenclature: 0 (Soil)
Entry Date(s): Date Created: 20260706 Date Completed: 20260804 Latest Revision: 20260805
Update Code: 20260806
DOI: 10.1093/jambio/lxag165
PMID: 42409358
Database: MEDLINE
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  Label: Title
  Group: Ti
  Data: Preliminary strain-specific and non-additive effects of single versus mixed DSE inoculation on rhizosphere microbiome and nutrient cycling relative to plant biomass in Chinese yam.
– Name: Author
  Label: Authors
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  Data: <searchLink fieldCode="AU" term="%22Su+Z%22">Su Z</searchLink>; College of Traditional Chinese Medicine, Hebei University, Baoding, Hebei 071000, China.<br /><searchLink fieldCode="AU" term="%22Li+M%22">Li M</searchLink>; College of Traditional Chinese Medicine, Hebei University, Baoding, Hebei 071000, China.<br /><searchLink fieldCode="AU" term="%22Zuo+Y%22">Zuo Y</searchLink>; College of Traditional Chinese Medicine, Hebei University, Baoding, Hebei 071000, China.; Hebei Administration of Traditional Chinese Medicine Collaborative Innovation Key Research Laboratory of Chinese Medicinal Resource Industrialization, Baoding, Hebei 071000, China.<br /><searchLink fieldCode="AU" term="%22Guo+J%22">Guo J</searchLink>; College of Traditional Chinese Medicine, Hebei University, Baoding, Hebei 071000, China.<br /><searchLink fieldCode="AU" term="%22Wei+Y%22">Wei Y</searchLink>; College of Traditional Chinese Medicine, Hebei University, Baoding, Hebei 071000, China.<br /><searchLink fieldCode="AU" term="%22Fan+S%22">Fan S</searchLink>; College of Traditional Chinese Medicine, Hebei University, Baoding, Hebei 071000, China.; Hebei Administration of Traditional Chinese Medicine Collaborative Innovation Key Research Laboratory of Chinese Medicinal Resource Industrialization, Baoding, Hebei 071000, China.<br /><searchLink fieldCode="AU" term="%22Wang+Y%22">Wang Y</searchLink>; School of Basic Medical Sciences, Hebei University, Baoding, Hebei 071000, China.; Hebei Administration of Traditional Chinese Medicine Collaborative Innovation Key Research Laboratory of Chinese Medicinal Resource Industrialization, Baoding, Hebei 071000, China.
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  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22Oxford+University+Press%22">Oxford University Press </searchLink><i>Country of Publication: </i>England <i>NLM ID: </i>9706280 <i>Publication Model: </i>Print <i>Cited Medium: </i>Internet <i>ISSN: </i>1365-2672 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2213645072%22">13645072 </searchLink><i>NLM ISO Abbreviation: </i>J Appl Microbiol <i>Subsets: </i>MEDLINE
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  Data: <i>Publication</i>: 2022- : Oxford : Oxford University Press<br /><i>Original Publication</i>: Oxford : Published for the Society for Applied Bacteriology by Blackwell Science, c1997-
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  Data: <searchLink fieldCode="MM" term="%22Dioscorea%22">Dioscorea*</searchLink>/<searchLink fieldCode="MM" term="%22Dioscorea+microbiology%22">microbiology</searchLink> <br /><searchLink fieldCode="MM" term="%22Dioscorea%22">Dioscorea*</searchLink>/<searchLink fieldCode="MM" term="%22Dioscorea+growth+%26+development%22">growth & development</searchLink> <br /><searchLink fieldCode="MM" term="%22Dioscorea%22">Dioscorea*</searchLink>/<searchLink fieldCode="MM" term="%22Dioscorea+metabolism%22">metabolism</searchLink> <br /><searchLink fieldCode="MM" term="%22Endophytes%22">Endophytes*</searchLink>/<searchLink fieldCode="MM" term="%22Endophytes+physiology%22">physiology</searchLink> <br /><searchLink fieldCode="MM" term="%22Endophytes%22">Endophytes*</searchLink>/<searchLink fieldCode="MM" term="%22Endophytes+isolation+%26+purification%22">isolation & purification</searchLink> <br /><searchLink fieldCode="MM" term="%22Endophytes%22">Endophytes*</searchLink>/<searchLink fieldCode="MM" term="%22Endophytes+genetics%22">genetics</searchLink> <br /><searchLink fieldCode="MM" term="%22Endophytes%22">Endophytes*</searchLink>/<searchLink fieldCode="MM" term="%22Endophytes+classification%22">classification</searchLink> <br /><searchLink fieldCode="MM" term="%22Rhizosphere%22">Rhizosphere*</searchLink> <br /><searchLink fieldCode="MM" term="%22Soil+Microbiology%22">Soil Microbiology*</searchLink> <br /><searchLink fieldCode="MM" term="%22Microbiota%22">Microbiota*</searchLink><br /><searchLink fieldCode="MH" term="%22Plant+Roots%22">Plant Roots</searchLink>/<searchLink fieldCode="MH" term="%22Plant+Roots+microbiology%22">microbiology</searchLink> ; <searchLink fieldCode="MH" term="%22Plant+Roots%22">Plant Roots</searchLink>/<searchLink fieldCode="MH" term="%22Plant+Roots+growth+%26+development%22">growth & development</searchLink> ; <searchLink fieldCode="MH" term="%22Bacteria%22">Bacteria</searchLink>/<searchLink fieldCode="MH" term="%22Bacteria+classification%22">classification</searchLink> ; <searchLink fieldCode="MH" term="%22Bacteria%22">Bacteria</searchLink>/<searchLink fieldCode="MH" term="%22Bacteria+isolation+%26+purification%22">isolation & purification</searchLink> ; <searchLink fieldCode="MH" term="%22Bacteria%22">Bacteria</searchLink>/<searchLink fieldCode="MH" term="%22Bacteria+genetics%22">genetics</searchLink> ; <searchLink fieldCode="MH" term="%22Bacteria%22">Bacteria</searchLink>/<searchLink fieldCode="MH" term="%22Bacteria+metabolism%22">metabolism</searchLink> ; <searchLink fieldCode="MH" term="%22Fungi%22">Fungi</searchLink>/<searchLink fieldCode="MH" term="%22Fungi+classification%22">classification</searchLink> ; <searchLink fieldCode="MH" term="%22Fungi%22">Fungi</searchLink>/<searchLink fieldCode="MH" term="%22Fungi+isolation+%26+purification%22">isolation & purification</searchLink> ; <searchLink fieldCode="MH" term="%22Fungi%22">Fungi</searchLink>/<searchLink fieldCode="MH" term="%22Fungi+genetics%22">genetics</searchLink> ; <searchLink fieldCode="MH" term="%22Fungi%22">Fungi</searchLink>/<searchLink fieldCode="MH" term="%22Fungi+metabolism%22">metabolism</searchLink> ; <searchLink fieldCode="MH" term="%22Soil%22">Soil</searchLink>/<searchLink fieldCode="MH" term="%22Soil+chemistry%22">chemistry</searchLink> ; <searchLink fieldCode="MH" term="%22Biomass%22">Biomass</searchLink> ; <searchLink fieldCode="MH" term="%22China%22">China</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Aims: To mitigate Chinese yam continuous cropping obstacles, this study assessed single and mixed dark septate endophyte (DSE) inoculation effects on yam growth, soil characteristics and rhizosphere microbial communities, offering references for optimizing rhizosphere microenvironment of continuously cropped medicinal plant soils.<br />Methods and Results: 9 DSE strains were isolated from yam roots for single/mixed seedling pot inoculation; We found that growth-promoting effects differed by strain. Acrocalymma and Setophoma terrestris were the most commonly isolated DSE species, but strains with lower isolation rates Paraphoma ledniceana, Amesia atrobrunnea, and Zopfiella pilifera exhibited the most pronounced positive effects on yam root biomass. Increasing the number of co-inoculated strains did not proportionally enhance yam growth but progressively restructured soil microbial communities. However, mixed DSE inoculation as a whole showed no obvious growth promotion compared with the control. Fungal co-occurrence networks exhibited higher modularity and clustering, while bacterial communities had greater connectivity, especially under P. ledniceana and Fusarium sp. inoculation. Biosynthesis was the most abundant predicted function of rhizosphere microbes, with bacteria predicted to be enriched in detoxification and fungi in degradation-related pathways. Two strains of Chaetomiaceae and Periconia epilithographicola increased soil pH, organic carbon and available phosphorus, while available nitrogen decreased after DSE inoculation. Importantly, P. epilithographicola inoculation enhanced mycorrhizal fungi and mitigated microbial imbalance.<br />Conclusions: Single and mixed DSE inoculations regulate rhizosphere microbial assembly and nutrient cycling via species-specific effects. Screening beneficial DSE consortia and characterizing their influence on rhizosphere networks provides preliminary theoretical insights and and candidate strains that may relieve yam replant obstacles in future applications.<br /> (© The Author(s) 2026. Published by Oxford University Press on behalf of Applied Microbiology International. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site-for further information please contact journals.permissions@oup.com.)
– Name: GrantInfo
  Label: Grant Information
  Group: Grant
  Data: C2024201069 Natural Science Foundation of Hebei Province; QN2025140 Natural Science Foundation of Hebei Provincial Universities; 20240031 Hebei University; 20240053 Hebei University; CXZZSS2025011 Innovation Fund Project of Hebei Province
– Name: SubjectMinor
  Label: Contributed Indexing
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  Data: <i>Keywords: </i>Chinese Yam; co-occurrence networks; dark septate endophytes; mixed microbial inoculation; rhizosphere interactions
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  Data: <i>Date Created: </i>20260706 <i>Date Completed: </i>20260804 <i>Latest Revision: </i>20260805
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  Data: 10.1093/jambio/lxag165
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  Data: 42409358
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=cmedm&AN=42409358
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        Value: 10.1093/jambio/lxag165
    Languages:
      – Code: eng
        Text: English
    Subjects:
      – SubjectFull: Plant Roots microbiology
        Type: general
      – SubjectFull: Plant Roots growth & development
        Type: general
      – SubjectFull: Bacteria classification
        Type: general
      – SubjectFull: Bacteria isolation & purification
        Type: general
      – SubjectFull: Bacteria genetics
        Type: general
      – SubjectFull: Bacteria metabolism
        Type: general
      – SubjectFull: Fungi classification
        Type: general
      – SubjectFull: Fungi isolation & purification
        Type: general
      – SubjectFull: Fungi genetics
        Type: general
      – SubjectFull: Fungi metabolism
        Type: general
      – SubjectFull: Soil chemistry
        Type: general
      – SubjectFull: Biomass
        Type: general
      – SubjectFull: China
        Type: general
      – SubjectFull: Dioscorea microbiology
        Type: general
      – SubjectFull: Dioscorea growth & development
        Type: general
      – SubjectFull: Dioscorea metabolism
        Type: general
      – SubjectFull: Endophytes physiology
        Type: general
      – SubjectFull: Endophytes isolation & purification
        Type: general
      – SubjectFull: Endophytes genetics
        Type: general
      – SubjectFull: Endophytes classification
        Type: general
      – SubjectFull: Rhizosphere
        Type: general
      – SubjectFull: Soil Microbiology
        Type: general
      – SubjectFull: Microbiota
        Type: general
    Titles:
      – TitleFull: Preliminary strain-specific and non-additive effects of single versus mixed DSE inoculation on rhizosphere microbiome and nutrient cycling relative to plant biomass in Chinese yam.
        Type: main
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              Text: 2026 Aug 04
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              Y: 2026
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