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Genomic characterization of antimicrobial resistance genes, mobile genetic elements, sequence types, and serotypes of Escherichia coli derived from water buffalo in Italy.

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Τίτλος: Genomic characterization of antimicrobial resistance genes, mobile genetic elements, sequence types, and serotypes of Escherichia coli derived from water buffalo in Italy.
Συγγραφείς: Rinaldi A; Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, Naples, Italy., Paradiso R; Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, Naples, Italy. Electronic address: rubina.paradiso@izsmportici.it., Sorbo LD; Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, Naples, Italy., Minerva M; Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, Naples, Italy., Cuomo MC; Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, Naples, Italy., Juliano S; Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, Naples, Italy., Paciello O; Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, Naples, Italy., Iovane G; Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, Naples, Italy., Borriello G; Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, Naples, Italy.
Πηγή: Journal of global antimicrobial resistance [J Glob Antimicrob Resist] 2026 Sep; Vol. 50, pp. 146-153. Date of Electronic Publication: 2026 Jul 01.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Published by Elsevier Ltd. on behalf of International Society of Chemotherapy for Infection and Cancer Country of Publication: Netherlands NLM ID: 101622459 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2213-7173 (Electronic) Linking ISSN: 22137165 NLM ISO Abbreviation: J Glob Antimicrob Resist Subsets: MEDLINE
Imprint Name(s): Original Publication: Amsterdam : Published by Elsevier Ltd. on behalf of International Society of Chemotherapy for Infection and Cancer
Ιατρικοί όροι (MeSH): Buffaloes*/microbiology , Escherichia coli*/genetics , Escherichia coli*/drug effects , Escherichia coli*/classification , Escherichia coli*/isolation & purification , Escherichia coli Infections*/veterinary , Escherichia coli Infections*/microbiology , Drug Resistance, Multiple, Bacterial*/genetics , Interspersed Repetitive Sequences*, Anti-Bacterial Agents/pharmacology ; Plasmids/genetics ; Virulence Factors/genetics ; Animals ; Italy ; Serogroup ; Whole Genome Sequencing ; Microbial Sensitivity Tests ; Genome, Bacterial ; Drug Resistance, Bacterial ; Genomics
Περίληψη: Objective: The spread of antimicrobial resistance (AMR) in Escherichia coli is driven by the acquisition of antibiotic resistance genes (ARGs) via mobile genetic elements in animal and environmental reservoirs. This study aimed to characterize the resistome, mobilome, and their associations in E. coli isolates from water buffaloes in southern Italy, evaluating their contribution to AMR dissemination.
Methods: Thirty E. coli isolates were collected from water buffaloes on 16 farms in the Campania region between 2016 and 2023. Whole-genome sequencing was performed, and assemblies were analysed to determine sequence types (STs), serotypes, virulence factors, mobile genetic elements, and ARGs using bioinformatic tools. Statistical analyses were applied to assess associations between resistance gene and isolation data.
Results: Genomic analysis identified 18 STs, with ST10, ST29, and ST58 most frequent. The mobilome included 155 plasmids, 1175 prophages, 29 integrons, and over 700 insertion sequences, reflecting high genomic plasticity. A total of 87 ARGs, conferring resistance to β-lactams, aminoglycosides, tetracyclines, and sulphonamides, were detected, and all isolates were multidrug-resistant. About 30% of resistance genes were linked to plasmids or integrons. Significant associations between specific ARGs and farm or sampling year suggest environmental and temporal influences.
Conclusions: E. coli from water buffaloes in Campania harbour a complex resistome and mobilome, supporting their role as reservoirs of AMR. The co-localization of multiple resistance genes on mobile elements highlights the risk of interspecies and zoonotic transmission, emphasizing the need for genomic surveillance within a One Health framework.
(Copyright © 2026 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
Contributed Indexing: Keywords: Antimicrobial resistance; Escherichia coli; Mobile genetic element; One Health; Water buffalo
Substance Nomenclature: 0 (Anti-Bacterial Agents)
0 (Virulence Factors)
Entry Date(s): Date Created: 20260701 Date Completed: 20260923 Latest Revision: 20260923
Update Code: 20260924
DOI: 10.1016/j.jgar.2026.06.015
PMID: 42385981
Βάση Δεδομένων: MEDLINE
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  Data: Genomic characterization of antimicrobial resistance genes, mobile genetic elements, sequence types, and serotypes of Escherichia coli derived from water buffalo in Italy.
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  Data: <searchLink fieldCode="AU" term="%22Rinaldi+A%22">Rinaldi A</searchLink>; Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, Naples, Italy.<br /><searchLink fieldCode="AU" term="%22Paradiso+R%22">Paradiso R</searchLink>; Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, Naples, Italy. Electronic address: rubina.paradiso@izsmportici.it.<br /><searchLink fieldCode="AU" term="%22Sorbo+LD%22">Sorbo LD</searchLink>; Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, Naples, Italy.<br /><searchLink fieldCode="AU" term="%22Minerva+M%22">Minerva M</searchLink>; Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, Naples, Italy.<br /><searchLink fieldCode="AU" term="%22Cuomo+MC%22">Cuomo MC</searchLink>; Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, Naples, Italy.<br /><searchLink fieldCode="AU" term="%22Juliano+S%22">Juliano S</searchLink>; Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, Naples, Italy.<br /><searchLink fieldCode="AU" term="%22Paciello+O%22">Paciello O</searchLink>; Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, Naples, Italy.<br /><searchLink fieldCode="AU" term="%22Iovane+G%22">Iovane G</searchLink>; Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, Naples, Italy.<br /><searchLink fieldCode="AU" term="%22Borriello+G%22">Borriello G</searchLink>; Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, Naples, Italy.
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  Data: <i>Original Publication</i>: Amsterdam : Published by Elsevier Ltd. on behalf of International Society of Chemotherapy for Infection and Cancer
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  Data: Objective: The spread of antimicrobial resistance (AMR) in Escherichia coli is driven by the acquisition of antibiotic resistance genes (ARGs) via mobile genetic elements in animal and environmental reservoirs. This study aimed to characterize the resistome, mobilome, and their associations in E. coli isolates from water buffaloes in southern Italy, evaluating their contribution to AMR dissemination.<br />Methods: Thirty E. coli isolates were collected from water buffaloes on 16 farms in the Campania region between 2016 and 2023. Whole-genome sequencing was performed, and assemblies were analysed to determine sequence types (STs), serotypes, virulence factors, mobile genetic elements, and ARGs using bioinformatic tools. Statistical analyses were applied to assess associations between resistance gene and isolation data.<br />Results: Genomic analysis identified 18 STs, with ST10, ST29, and ST58 most frequent. The mobilome included 155 plasmids, 1175 prophages, 29 integrons, and over 700 insertion sequences, reflecting high genomic plasticity. A total of 87 ARGs, conferring resistance to β-lactams, aminoglycosides, tetracyclines, and sulphonamides, were detected, and all isolates were multidrug-resistant. About 30% of resistance genes were linked to plasmids or integrons. Significant associations between specific ARGs and farm or sampling year suggest environmental and temporal influences.<br />Conclusions: E. coli from water buffaloes in Campania harbour a complex resistome and mobilome, supporting their role as reservoirs of AMR. The co-localization of multiple resistance genes on mobile elements highlights the risk of interspecies and zoonotic transmission, emphasizing the need for genomic surveillance within a One Health framework.<br /> (Copyright © 2026 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
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