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Ecological and evolutionary implications of a mobile genetic element-rich haloarchaeon with unique osmotic resilience.

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Τίτλος: Ecological and evolutionary implications of a mobile genetic element-rich haloarchaeon with unique osmotic resilience.
Συγγραφείς: Ni Y; College of Food Science and Technology, Shanghai Ocean University, Shanghai, China.; Laboratory for Marine Biology and Biotechnology, Qingdao Marine Science and Technology Center, Qingdao, China., Bi W; College of Food Science and Technology, Shanghai Ocean University, Shanghai, China., Yu H; College of Food Science and Technology, Shanghai Ocean University, Shanghai, China., Chen L; College of Food Science and Technology, Shanghai Ocean University, Shanghai, China., Yu Y; College of Food Science and Technology, Shanghai Ocean University, Shanghai, China., Han J; Shanghai Wildlife and Protected Natural Areas Research Center, Shanghai, China., Wang Y; College of Food Science and Technology, Shanghai Ocean University, Shanghai, China.; Laboratory for Marine Biology and Biotechnology, Qingdao Marine Science and Technology Center, Qingdao, China.
Πηγή: Microbiology spectrum [Microbiol Spectr] 2026 Sep; Vol. 14 (9), pp. e0340325. Date of Electronic Publication: 2026 Jun 30.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: ASM Press Country of Publication: United States NLM ID: 101634614 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2165-0497 (Electronic) Linking ISSN: 21650497 NLM ISO Abbreviation: Microbiol Spectr Subsets: MEDLINE
Imprint Name(s): Original Publication: Washington, DC : ASM Press, 2013-
Ιατρικοί όροι (MeSH): Halobacteriaceae*/genetics , Halobacteriaceae*/classification , Halobacteriaceae*/isolation & purification , Interspersed Repetitive Sequences*, Lakes/microbiology ; Plasmids/genetics ; Phylogeny ; Gene Transfer, Horizontal ; China ; Genome, Archaeal ; Salinity ; Osmotic Pressure ; Evolution, Molecular ; Adaptation, Physiological
Περίληψη: We isolated a novel halophilic archaeon, strain DSL9, representing the proposed new species Haloliberatus hailidukes gen. nov., sp. nov., from Dishui Lake, China. Unlike most obligate halophiles, DSL9 survives in low salinity, even distilled water, without lysis. Genomic analysis revealed dual salinity adaptation strategies: salt-in and compatible solutes, including a complete trehalose biosynthesis pathway. The strain harbors multiple plasmids, notably a 111,311 bp large plasmid (pHdsl9-3) encoding replication (Orc1/Cdc6, SSB), transcription (TFIIB), transmission (T4SS cluster, ArdC-like protein), and recombination (XerA) modules. pHdsl9-3 provides auxiliary functions such as defense, genome diversification, ion detoxification, and suggests active horizontal gene transfer. Similar elements are widespread in Halobacteriales, highlighting their role in haloarchaeal genetic diversity and plasticity. The encoded XerA hinted at a function beyond DNA dimer resolution, suggesting it may have been adapted by other archaeal mobile genetic elements. These findings underscore the need to investigate plasmid-driven evolution and environmental adaptation mechanisms in haloarchaea.IMPORTANCEThis study reports the isolation and characterization of DSL9, a novel halophilic archaeon from a freshwater lake. Remarkably, DSL9 defies the typical obligate halophilic lifestyle by surviving in low-salinity environments, including distilled water, without cell lysis. A key discovery is the identification of a 111,311 bp large plasmid harboring essential modules for replication, transcription, transmission, and integration. Widespread distribution of similar elements across Halobacteriales suggests their crucial role in haloarchaeal genetic diversity and plasticity, warranting further study of plasmid-mediated evolution and adaptation strategies.
Competing Interests: The authors declare no conflict of interest.
References: J Bacteriol. 2006 Dec;188(23):8136-44. (PMID: 16997958)
Environ Microbiol Rep. 2024 Dec;16(6):e70039. (PMID: 39568122)
PLoS Comput Biol. 2017 Jun 8;13(6):e1005595. (PMID: 28594827)
Biochem Soc Trans. 2004 Apr;32(Pt 2):222-6. (PMID: 15046576)
Antonie Van Leeuwenhoek. 2021 Jan;114(1):83-94. (PMID: 33389352)
Nucleic Acids Res. 2008 Jul 1;36(Web Server issue):W5-9. (PMID: 18440982)
Nat Rev Microbiol. 2021 Jun;19(6):347-359. (PMID: 33469168)
Trends Biotechnol. 2024 Aug;42(8):970-985. (PMID: 38443218)
Int J Syst Evol Microbiol. 2007 Feb;57(Pt 2):387-392. (PMID: 17267984)
Int J Syst Evol Microbiol. 2008 Aug;58(Pt 8):1896-902. (PMID: 18676476)
Subcell Biochem. 2019;92:471-493. (PMID: 31214995)
Biochim Biophys Acta. 2009 Aug;1791(8):719-29. (PMID: 19303051)
Chem Rev. 2016 Oct 26;116(20):12785-12820. (PMID: 27163859)
BMC Bioinformatics. 2019 Sep 14;20(1):473. (PMID: 31521110)
PLoS Genet. 2020 Apr 29;16(4):e1008750. (PMID: 32348296)
Nat Biotechnol. 2017 Nov;35(11):1026-1028. (PMID: 29035372)
Front Microbiol. 2019 Aug 27;10:1928. (PMID: 31507553)
Sci Rep. 2020 Feb 24;10(1):3298. (PMID: 32094390)
Microbiol Spectr. 2014 Dec;2(6):. (PMID: 26104461)
Microbiol Mol Biol Rev. 2007 Mar;71(1):121-57. (PMID: 17347521)
Nucleic Acids Res. 2021 Nov 8;49(19):10868-10878. (PMID: 34606606)
Microbiologyopen. 2014 Oct;3(5):688-701. (PMID: 25060695)
Nat Biotechnol. 2024 Feb;42(2):243-246. (PMID: 37156916)
Nat Rev Microbiol. 2024 Mar;22(3):170-185. (PMID: 37814112)
Curr Opin Microbiol. 2015 Jun;25:120-6. (PMID: 26066288)
Extremophiles. 2012 Nov;16(6):895-901. (PMID: 23052830)
Nature. 2013 Nov 28;503(7477):544-547. (PMID: 24185008)
Nucleic Acids Res. 2023 Apr 24;51(7):3270-3287. (PMID: 36864746)
Nat Rev Microbiol. 2014 Sep;12(9):635-45. (PMID: 25118885)
Bioinformatics. 2017 Nov 01;33(21):3340-3347. (PMID: 29077810)
Nucleic Acids Res. 2021 Jul 2;49(W1):W293-W296. (PMID: 33885785)
Nature. 2022 Jul;607(7917):191-196. (PMID: 35732732)
Microbiol Mol Biol Rev. 2012 Sep;76(3):565-84. (PMID: 22933560)
Saline Syst. 2008 Aug 29;4:13. (PMID: 18759987)
Int J Syst Evol Microbiol. 2012 Aug;62(Pt 8):1932-1936. (PMID: 22003044)
Front Microbiol. 2023 Sep 18;14:1274068. (PMID: 37789858)
Int J Syst Evol Microbiol. 2024 Mar;74(3):. (PMID: 38456846)
J Bacteriol. 2006 Oct;188(19):6915-23. (PMID: 16980494)
Syst Appl Microbiol. 2022 Jul;45(4):126336. (PMID: 35644061)
EcoSal Plus. 2016 Oct;7(1):. (PMID: 27735785)
Int J Syst Evol Microbiol. 2008 Sep;58(Pt 9):2031-5. (PMID: 18768599)
FEMS Microbiol Rev. 2018 May 1;42(3):353-375. (PMID: 29529204)
Microbiol Spectr. 2014 Dec;2(6):. (PMID: 26104463)
Nucleic Acids Res. 2022 Jan 7;50(D1):D785-D794. (PMID: 34520557)
Nat Microbiol. 2017 Oct;2(10):1446-1455. (PMID: 28827601)
FEMS Microbiol Rev. 2021 Aug 17;45(4):. (PMID: 33524101)
PLoS Genet. 2017 Jun 19;13(6):e1006847. (PMID: 28628615)
Int J Syst Evol Microbiol. 2019 May;69(5):1306-1312. (PMID: 30789324)
Bacteriol Rev. 1974 Sep;38(3):272-90. (PMID: 4607500)
Int J Syst Evol Microbiol. 2007 Jan;57(Pt 1):81-91. (PMID: 17220447)
Mar Life Sci Technol. 2021 Jan 11;3(2):243-251. (PMID: 37073340)
Nat Methods. 2015 Jan;12(1):59-60. (PMID: 25402007)
Plasmid. 2016 Jan;83:1-7. (PMID: 26601892)
Int J Syst Evol Microbiol. 2023 Nov;73(11):. (PMID: 37917544)
Saline Syst. 2007 Mar 09;3:2. (PMID: 17346353)
Microbiol Spectr. 2015 Apr;3(2):MDNA3-0030-2014. (PMID: 26104715)
Int J Syst Evol Microbiol. 2010 May;60(Pt 5):1196-1199. (PMID: 19667389)
Microorganisms. 2023 Dec 19;12(1):. (PMID: 38276173)
PLoS Genet. 2010 Oct 21;6(10):e1001166. (PMID: 20975945)
Front Microbiol. 2017 Mar 20;8:453. (PMID: 28373867)
BMC Bioinformatics. 2010 Mar 08;11:119. (PMID: 20211023)
Mol Biol Evol. 2020 Jun 1;37(6):1727-1743. (PMID: 32068866)
Nucleic Acids Res. 2021 Sep 20;49(16):9077-9096. (PMID: 34417604)
Life Sci. 2024 Aug 1;350:122672. (PMID: 38705456)
Bioinformatics. 2021 Aug 25;37(16):2473-2475. (PMID: 33459763)
Int J Syst Evol Microbiol. 2023 May;73(5):. (PMID: 37252776)
Nature. 2014 Oct 23;514(7523):518-22. (PMID: 25132545)
Nat Commun. 2022 May 10;13(1):2561. (PMID: 35538097)
Can J Microbiol. 1978 Jun;24(6):710-5. (PMID: 667737)
Mol Biol Evol. 2009 Jul;26(7):1641-50. (PMID: 19377059)
Genomics. 2008 Apr;91(4):335-46. (PMID: 18313895)
Grant Information: 32370151 National Natural Science Foundation of China
Contributed Indexing: Keywords: halophilic archaeon; horizontal gene transfer; low salinity; megaplasmid
Entry Date(s): Date Created: 20260630 Date Completed: 20260902 Latest Revision: 20260903
Update Code: 20260903
PubMed Central ID: PMC13532300
DOI: 10.1128/spectrum.03403-25
PMID: 42379816
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:2165-0497
DOI:10.1128/spectrum.03403-25