Academic Journal
LncRNA MIAT Protects Against Sevoflurane-Induced Cognitive Dysfunction in Neonatal Rats via the miR-15b-5p/Ccnd1 Axis.
| Title: | LncRNA MIAT Protects Against Sevoflurane-Induced Cognitive Dysfunction in Neonatal Rats via the miR-15b-5p/Ccnd1 Axis. |
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| Authors: | Lu X; Anesthesiology Department, Zhongda Hospital (Jiangbei), Southeast University, Nanjing, Jiangsu, China., He X; Anesthesiology Department, First Affiliated Hospital of Kunming Medical University, Kunming, China., Zhan Z; Anesthesiology Department, The First Hospital of Putian City, Putian, China., Chen H; Anesthesiology Department, The First Hospital of Putian City, Putian, China., Chen J; Department of Cardiovascular Medicine, The First Hospital of Putian City, Putian, China., Fan Z; Department of Anesthesiology, Caidian District People's Hospital of Wuhan, Wuhan, China. |
| Source: | Synapse (New York, N.Y.) [Synapse] 2026 Jul; Vol. 80 (4), pp. e70050. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Country of Publication: United States NLM ID: 8806914 Publication Model: Print Cited Medium: Internet ISSN: 1098-2396 (Electronic) Linking ISSN: 08874476 NLM ISO Abbreviation: Synapse Subsets: MEDLINE |
| Imprint Name(s): | Publication: <2005-> : Hoboken, N.J. : Wiley Original Publication: New York : Alan R. Liss, Inc., c1987- |
| MeSH Terms: | Sevoflurane*/toxicity , MicroRNAs*/metabolism , MicroRNAs*/genetics , RNA, Long Noncoding*/metabolism , RNA, Long Noncoding*/genetics , Cyclin D1*/metabolism , Cyclin D1*/genetics , Cognitive Dysfunction*/chemically induced , Cognitive Dysfunction*/metabolism , Cognitive Dysfunction*/prevention & control , Anesthetics, Inhalation*/toxicity, Hippocampus/metabolism ; Hippocampus/drug effects ; Animals ; Rats, Sprague-Dawley ; Animals, Newborn ; Rats ; Male |
| Abstract: | This study aimed to investigate the role and underlying mechanism of lncRNA MIAT in sevoflurane (Sev)-induced cognitive dysfunction in neonatal rats, thereby offering theoretical basis for clinical intervention. Seven-day-old Sprague-Dawley (SD) rats were chosen to construct a Sev-induced cognitive dysfunction model. Anxiety-like behavior and locomotor activity were assessed by the open field test (OFT), whereas cognitive function was evaluated using novel object recognition (NOR) test and Morris water maze (MWM) test. The expression levels of lncRNA MIAT, miR-15b-5p, and Ccnd1 were detected by RT-qPCR. The dual-luciferase reporter gene assay was carried out to validate the targeted binding relationships. Sev exposure led to a downregulation of lncRNA MIAT expression in rat hippocampus. lncRNA MIAT alleviated Sev-induced cognitive impairment, as manifested by increased central zone residence time in OFT, elevated recognition index (RI) in NOR test, shortened escape latency, increased platform crossings, and prolonged target quadrant residence time in MWM test. Mechanistically, lncRNA MIAT directly targeted miR-15b-5p and inhibited its expression. miR-15b-5p targeted Ccnd1 and suppressed its expression. Overexpression of miR-15b-5p or silencing of Ccnd1 reversed the neuroprotective effect of lncRNA MIAT. LncRNA MIAT ameliorates Sev-induced cognitive dysfunction in neonatal rats by sponging miR-15b-5p to upregulate Ccnd1 expression, which may serve as a potential target for preventing Sev-related neurotoxicity. (© 2026 Wiley Periodicals LLC.) |
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| Contributed Indexing: | Keywords: Ccnd1; LncRNA MIAT; cognitive dysfunction; miR‐15b‐5p; sevoflurane |
| Substance Nomenclature: | 38LVP0K73A (Sevoflurane) 0 (MicroRNAs) 0 (RNA, Long Noncoding) 136601-57-5 (Cyclin D1) 0 (Miat long non-coding RNA) 0 (mirn15 microRNA, rat) 0 (Ccnd1 protein, rat) 0 (Anesthetics, Inhalation) |
| Entry Date(s): | Date Created: 20260630 Date Completed: 20260630 Latest Revision: 20260630 |
| Update Code: | 20260630 |
| DOI: | 10.1002/syn.70050 |
| PMID: | 42374622 |
| Database: | MEDLINE |
| ISSN: | 1098-2396 |
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| DOI: | 10.1002/syn.70050 |