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Optimized SPE-HPLC-FLD Method for the Simultaneous Determination of Olaparib, Propranolol, and Furosemide in Human Urine.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος: Optimized SPE-HPLC-FLD Method for the Simultaneous Determination of Olaparib, Propranolol, and Furosemide in Human Urine.
Συγγραφείς: Kamaris G; Laboratory of Pharmaceutical Analysis, Department of Pharmacy, Aristotle University of Thessaloniki, Thessaloniki, Greece., Kalagia A; Laboratory of Pharmaceutical Analysis, Department of Pharmacy, Aristotle University of Thessaloniki, Thessaloniki, Greece., Markopoulou CK; Laboratory of Pharmaceutical Analysis, Department of Pharmacy, Aristotle University of Thessaloniki, Thessaloniki, Greece.
Πηγή: Biomedical chromatography : BMC [Biomed Chromatogr] 2026 Aug; Vol. 40 (8), pp. e70529.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Wiley Country of Publication: England NLM ID: 8610241 Publication Model: Print Cited Medium: Internet ISSN: 1099-0801 (Electronic) Linking ISSN: 02693879 NLM ISO Abbreviation: Biomed Chromatogr Subsets: MEDLINE
Imprint Name(s): Publication: 1990- : Chichester : Wiley
Original Publication: London : Heyden & Son, c1986-1990
Ιατρικοί όροι (MeSH): Solid Phase Extraction*/methods , Furosemide*/urine , Furosemide*/isolation & purification , Furosemide*/chemistry , Propranolol*/urine , Propranolol*/isolation & purification , Propranolol*/chemistry , Piperazines*/urine , Piperazines*/isolation & purification , Piperazines*/chemistry , Phthalazines*/urine , Phthalazines*/isolation & purification , Phthalazines*/chemistry, Chromatography, High Pressure Liquid/methods ; Humans ; Reproducibility of Results ; Linear Models ; Limit of Detection
Περίληψη: Olaparib belongs to the PARP inhibitors and is widely used as an anticancer drug. Due to frequent side effects, it is usually co-administered with an antihypertensive, such as propranolol and/or an antidiuretic, such as furosemide. In this study, a highly sensitive HPLC-FLD method was developed for the simultaneous determination of the three APIs in urine. For their analysis, a Nucleosil C8 column (250 mm × 4.6 mm, 5 μm) at 50°C was used as the stationary phase and an isocratic system of methanol-acetonitrile-20 mM NaH2PO4 at pH 3.6, 12:20:68 v/v/v as the mobile phase. The calibration curves for the analytes were generated both in urine and in the pure diluent. The reliability of the method was evaluated according to ICH guidelines in terms of accuracy and repeatability. For sample purification, protein precipitation techniques involving the addition of organic solvents, acids, or salts, as well as the freezing-out technique, were studied. Liquid-liquid extraction, using various solvents, and solid-phase extraction (SPE) were also tested, where the latter was found more suitable. SPE optimization was achieved using experimental design and a D-optimal mixture design. Reliable analyte recoveries demonstrate the capability of the proposed method, making it a suitable tool for biological studies.
(© 2026 The Author(s). Biomedical Chromatography published by John Wiley & Sons Ltd.)
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Contributed Indexing: Keywords: furosemide; olaparib; propranolol; solid‐phase extraction (SPE); urine
Substance Nomenclature: 7LXU5N7ZO5 (Furosemide)
9Y8NXQ24VQ (Propranolol)
0 (Piperazines)
WOH1JD9AR8 (olaparib)
0 (Phthalazines)
Entry Date(s): Date Created: 20260626 Date Completed: 20260626 Latest Revision: 20260726
Update Code: 20260726
PubMed Central ID: PMC13306917
DOI: 10.1002/bmc.70529
PMID: 42359709
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:1099-0801
DOI:10.1002/bmc.70529