Λεπτομέρειες βιβλιογραφικής εγγραφής
| Τίτλος: |
Dietary α-Tocopherol Deficiency Disrupts Hepatic Circadian Clock and Lipid Metabolism in Association with Gut Microbiota Dysbiosis. |
| Συγγραφείς: |
Peng L; Yunnan Key Laboratory of Precision Nutrition and Personalized Food Manufacturing, Yunnan Agricultural University, Kunming 650201, China.; Engineering Research Center of Development and Utilization of Food and Drug Homologous Resources, Ministry of Education, Yunnan Agricultural University, Kunming 650201, China.; College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China., Zhao Y; Division of Science and Technology, Yunnan Agricultural University, Kunming 650201, China., Fan Y; Yunnan Key Laboratory of Precision Nutrition and Personalized Food Manufacturing, Yunnan Agricultural University, Kunming 650201, China.; Engineering Research Center of Development and Utilization of Food and Drug Homologous Resources, Ministry of Education, Yunnan Agricultural University, Kunming 650201, China.; College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China., Peng Q; Yunnan Key Laboratory of Precision Nutrition and Personalized Food Manufacturing, Yunnan Agricultural University, Kunming 650201, China.; Engineering Research Center of Development and Utilization of Food and Drug Homologous Resources, Ministry of Education, Yunnan Agricultural University, Kunming 650201, China.; College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China., Sheng J; College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China., Tian Y; Yunnan Key Laboratory of Precision Nutrition and Personalized Food Manufacturing, Yunnan Agricultural University, Kunming 650201, China.; Engineering Research Center of Development and Utilization of Food and Drug Homologous Resources, Ministry of Education, Yunnan Agricultural University, Kunming 650201, China.; College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China., Gao X; Yunnan Key Laboratory of Precision Nutrition and Personalized Food Manufacturing, Yunnan Agricultural University, Kunming 650201, China.; Engineering Research Center of Development and Utilization of Food and Drug Homologous Resources, Ministry of Education, Yunnan Agricultural University, Kunming 650201, China.; College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China. |
| Πηγή: |
Nutrients [Nutrients] 2026 Jun 09; Vol. 18 (12). Date of Electronic Publication: 2026 Jun 09. |
| Τύπος έκδοσης: |
Journal Article |
| Γλώσσα: |
English |
| Στοιχεία περιοδικού: |
Publisher: MDPI Publishing Country of Publication: Switzerland NLM ID: 101521595 Publication Model: Electronic Cited Medium: Internet ISSN: 2072-6643 (Electronic) Linking ISSN: 20726643 NLM ISO Abbreviation: Nutrients Subsets: MEDLINE |
| Imprint Name(s): |
Original Publication: Basel, Switzerland : MDPI Publishing |
| Ιατρικοί όροι (MeSH): |
Lipid Metabolism*/genetics , alpha-Tocopherol*/administration & dosage , Liver*/metabolism , Dysbiosis*/metabolism , Circadian Clocks*/physiology , Vitamin E Deficiency*/metabolism , Vitamin E Deficiency*/complications , Gastrointestinal Microbiome*, Animals ; Mice, Inbred C57BL ; Mice ; Male ; Diet |
| Περίληψη: |
Background/Objectives As a fat-soluble vitamin, vitamin E (VE) is prone to suboptimal intake in the general population. Alpha-tocopherol (α-TE) represents the most biologically significant form of VE in vivo. Nevertheless, the potential detrimental effects of α-TE deficiency on health remain unclear. This study was conducted to investigate the effect of α-TE deficiency on hepatic metabolism and gut microbiota. Methods C57BL/6J mice were randomly assigned to receive one of three dietary regimens: a α-TE-deficient diet, a control diet with normal α-TE, or a high-dose diet containing four times the normal α-TE level. Histopathology, serum biochemistry, RNA-Seq, RT-qPCR, Western blot, and 16S rRNA gene sequencing with correlation analysis were used to assess metabolic phenotypes, hepatic circadian, hepatic lipid metabolism, and cecal microbiota, respectively. Results The results demonstrated that α-TE deficiency induced hepatic steatosis and lipid metabolic disturbances. α-TE deficiency significantly decreased Arntl and Clock expression, but increased Per2. Additionally, it upregulated the expression of lipogenic genes such as Scd1, Elovl6, and Elovl3 and simultaneously downregulated fatty acid oxidation genes such as Cyp4a10, Cyp4a14, and Acot1, bringing about imbalance in lipid homeostasis. In addition, α-TE deficiency greatly changed the structure and composition of gut microbiota. Bacterial genera like Alistipes, norank_f__Muribaculaceae, Muribaculum, Odoribacter, and Dubosiella were significantly correlated with hepatic circadian and lipid metabolism gene expression with the strongest correlation being Alistipes. Conclusions This work is the first to reveal that short term α-TE deficiency could cause lipid metabolic disorder via the "gut microbiota-liver circadian clock" axis, which provides novel insights into the etiology of nutrition-related metabolic diseases and targets for nutritional intervention. |
| Grant Information: |
Key Project, 202301BD070001-026 Yunnan Agricultural Joint Special Project; Young talents project, 2022-0255 Xingdian talent plan of Yunnan Province; 202401AT070217 Yunnan Fundamental Research Projects |
| Contributed Indexing: |
Keywords: Alistipes; Bmal1; Per2; Scd1; alpha-tocopherol; circadian clock; clock; lipid metabolism; vitamin |
| Substance Nomenclature: |
H4N855PNZ1 (alpha-Tocopherol) |
| Entry Date(s): |
Date Created: 20260626 Date Completed: 20260626 Latest Revision: 20260726 |
| Update Code: |
20260726 |
| PubMed Central ID: |
PMC13305107 |
| DOI: |
10.3390/nu18121853 |
| PMID: |
42356241 |
| Βάση Δεδομένων: |
MEDLINE |