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Sulfide-mediated anammox performance under antibiotics stress: Linking antibiotic resistance genes, functional microbes and nitrogen-sulfur metabolism.

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Τίτλος: Sulfide-mediated anammox performance under antibiotics stress: Linking antibiotic resistance genes, functional microbes and nitrogen-sulfur metabolism.
Συγγραφείς: Wang J; National and Local Joint Engineering Laboratory of Municipal Sewage Resource Utilization Technology, Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment, School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, PR China., Wen J; National and Local Joint Engineering Laboratory of Municipal Sewage Resource Utilization Technology, Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment, School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, PR China., Zhang X; National and Local Joint Engineering Laboratory of Municipal Sewage Resource Utilization Technology, Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment, School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, PR China., Zhang X; Collaborative Innovation Center of Atmospheric Environment and Equipment Technology, Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control, School of Environmental Science and Engineering, Nanjing University of Information Science and Technology, Nanjing 210044, PR China., Wu P; National and Local Joint Engineering Laboratory of Municipal Sewage Resource Utilization Technology, Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment, School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, PR China. Electronic address: wupengniu@126.com.
Πηγή: Bioresource technology [Bioresour Technol] 2026 Nov; Vol. 459, pp. 135244. Date of Electronic Publication: 2026 Jun 25.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Elsevier Applied Science Country of Publication: England NLM ID: 9889523 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2976 (Electronic) Linking ISSN: 09608524 NLM ISO Abbreviation: Bioresour Technol Subsets: MEDLINE
Imprint Name(s): Original Publication: Barking, Essex, England : New York, N.Y. : Elsevier Applied Science ; Elsevier Science Pub. Co., 1991-
Ιατρικοί όροι (MeSH): Anti-Bacterial Agents*/pharmacology , Sulfides*/pharmacology , Nitrogen*/metabolism , Sulfur*/metabolism , Genes, Bacterial*/genetics , Stress, Physiological*/drug effects , Drug Resistance, Microbial*/genetics , Drug Resistance, Microbial*/drug effects , Anaerobic Ammonia Oxidation*/drug effects , Bacteria*/metabolism , Bacteria*/genetics , Bacteria*/drug effects , Ammonium Compounds*/metabolism, Sulfamethoxazole/pharmacology ; Oxytetracycline/pharmacology ; Oxidation-Reduction
Περίληψη: Anaerobic ammonium oxidation (anammox), a sustainable and energy-efficient biological nitrogen removal process, is vulnerable to antibiotic stress during stable operation, while the mechanism of functional recovery mediated by sulfides remains unclear. This study systematically analyzed the response characteristics of the anammox process under sustained high-concentration oxytetracycline (OTC) and sulfamethoxazole (SMX) exposure, and further evaluated the potential of sulfide as an exogenous regulatory factor to mitigate antibiotic stress. Results indicate that sustained high concentration OTC and SMX exposure impaired the system's nitrogen removal performance, while the removal efficiencies of ammonium and nitrite successfully recovered to 85% and 83%, respectively, following sulfide addition. Metagenomic analysis suggested that the addition of sulfide was accompanied by an increased abundance of potential genes related to sulfur and nitrogen metabolism. Moreover, sulfide may alleviate antibiotic stress by facilitating metabolic interactions related to electron transfer and increasing the potential for SMX degradation. Furthermore, under OTC and SMX stress, a 20% increase in the abundance of Brocadia sapporoensis harboring ARGs was closely associated with the addition of sulfide. This study elucidates the biological mechanisms by which sulfides mitigate antibiotic stress, providing a theoretical basis for recovery strategies of anammox under an intensified stress model.
(Copyright © 2026 Elsevier Ltd. All rights reserved.)
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Contributed Indexing: Keywords: Anammox; Antibiotic stress; Brocadia sapporoensis; Metabolism; Sulfide
Substance Nomenclature: 0 (Anti-Bacterial Agents)
0 (Sulfides)
N762921K75 (Nitrogen)
70FD1KFU70 (Sulfur)
JE42381TNV (Sulfamethoxazole)
X20I9EN955 (Oxytetracycline)
0 (Ammonium Compounds)
Entry Date(s): Date Created: 20260625 Date Completed: 20260821 Latest Revision: 20260821
Update Code: 20260821
DOI: 10.1016/j.biortech.2026.135244
PMID: 42349567
Βάση Δεδομένων: MEDLINE
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  Data: Sulfide-mediated anammox performance under antibiotics stress: Linking antibiotic resistance genes, functional microbes and nitrogen-sulfur metabolism.
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  Data: <searchLink fieldCode="AU" term="%22Wang+J%22">Wang J</searchLink>; National and Local Joint Engineering Laboratory of Municipal Sewage Resource Utilization Technology, Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment, School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, PR China.<br /><searchLink fieldCode="AU" term="%22Wen+J%22">Wen J</searchLink>; National and Local Joint Engineering Laboratory of Municipal Sewage Resource Utilization Technology, Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment, School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, PR China.<br /><searchLink fieldCode="AU" term="%22Zhang+X%22">Zhang X</searchLink>; National and Local Joint Engineering Laboratory of Municipal Sewage Resource Utilization Technology, Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment, School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, PR China.<br /><searchLink fieldCode="AU" term="%22Zhang+X%22">Zhang X</searchLink>; Collaborative Innovation Center of Atmospheric Environment and Equipment Technology, Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control, School of Environmental Science and Engineering, Nanjing University of Information Science and Technology, Nanjing 210044, PR China.<br /><searchLink fieldCode="AU" term="%22Wu+P%22">Wu P</searchLink>; National and Local Joint Engineering Laboratory of Municipal Sewage Resource Utilization Technology, Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment, School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, PR China. Electronic address: wupengniu@126.com.
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  Data: <i>Original Publication</i>: Barking, Essex, England : New York, N.Y. : Elsevier Applied Science ; Elsevier Science Pub. Co., 1991-
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  Label: Abstract
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  Data: Anaerobic ammonium oxidation (anammox), a sustainable and energy-efficient biological nitrogen removal process, is vulnerable to antibiotic stress during stable operation, while the mechanism of functional recovery mediated by sulfides remains unclear. This study systematically analyzed the response characteristics of the anammox process under sustained high-concentration oxytetracycline (OTC) and sulfamethoxazole (SMX) exposure, and further evaluated the potential of sulfide as an exogenous regulatory factor to mitigate antibiotic stress. Results indicate that sustained high concentration OTC and SMX exposure impaired the system's nitrogen removal performance, while the removal efficiencies of ammonium and nitrite successfully recovered to 85% and 83%, respectively, following sulfide addition. Metagenomic analysis suggested that the addition of sulfide was accompanied by an increased abundance of potential genes related to sulfur and nitrogen metabolism. Moreover, sulfide may alleviate antibiotic stress by facilitating metabolic interactions related to electron transfer and increasing the potential for SMX degradation. Furthermore, under OTC and SMX stress, a 20% increase in the abundance of Brocadia sapporoensis harboring ARGs was closely associated with the addition of sulfide. This study elucidates the biological mechanisms by which sulfides mitigate antibiotic stress, providing a theoretical basis for recovery strategies of anammox under an intensified stress model.<br /> (Copyright © 2026 Elsevier Ltd. All rights reserved.)
– Name: Abstract
  Label: Competing Interests
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  Data: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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  Data: <i>Keywords: </i>Anammox; Antibiotic stress; Brocadia sapporoensis; Metabolism; Sulfide
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  Data: 0 (Anti-Bacterial Agents)<br />0 (Sulfides)<br />N762921K75 (Nitrogen)<br />70FD1KFU70 (Sulfur)<br />JE42381TNV (Sulfamethoxazole)<br />X20I9EN955 (Oxytetracycline)<br />0 (Ammonium Compounds)
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        Value: 10.1016/j.biortech.2026.135244
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      – Code: eng
        Text: English
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        StartPage: 135244
    Subjects:
      – SubjectFull: Sulfamethoxazole pharmacology
        Type: general
      – SubjectFull: Oxytetracycline pharmacology
        Type: general
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      – SubjectFull: Stress, Physiological drug effects
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      – SubjectFull: Anaerobic Ammonia Oxidation drug effects
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      – SubjectFull: Bacteria genetics
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      – SubjectFull: Bacteria drug effects
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      – SubjectFull: Ammonium Compounds metabolism
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      – TitleFull: Sulfide-mediated anammox performance under antibiotics stress: Linking antibiotic resistance genes, functional microbes and nitrogen-sulfur metabolism.
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              Text: 2026 Nov
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