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Clarin-1 mRNA expression in the central auditory system of Fischer Brown Norway rats.

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Title: Clarin-1 mRNA expression in the central auditory system of Fischer Brown Norway rats.
Authors: Almassri LS; Department of Biomedical Sciences, Northeast Ohio Medical University, Rootstown, OH USA; University Hospitals | NEOMED Hearing Research Center, Northeast Ohio Medical University, Rootstown, OH USA. Electronic address: lalmassri@neomed.edu., Singh G; Department of Biomedical Sciences, Northeast Ohio Medical University, Rootstown, OH USA. Electronic address: gs169@uakron.edu., Barach GL; Department of Biomedical Sciences, Northeast Ohio Medical University, Rootstown, OH USA; University Hospitals | NEOMED Hearing Research Center, Northeast Ohio Medical University, Rootstown, OH USA. Electronic address: gbarach@neomed.edu., Fernandez M; Department of Biomedical Sciences, Northeast Ohio Medical University, Rootstown, OH USA. Electronic address: mef97@uakron.edu., Hamed H; Department of Biomedical Sciences, Northeast Ohio Medical University, Rootstown, OH USA; University Hospitals | NEOMED Hearing Research Center, Northeast Ohio Medical University, Rootstown, OH USA. Electronic address: hhamed@neomed.edu., Young JW; Department of Biomedical Sciences, Northeast Ohio Medical University, Rootstown, OH USA. Electronic address: jwyoung@neomed.edu., Alagramam KN; University Hospitals | NEOMED Hearing Research Center, Northeast Ohio Medical University, Rootstown, OH USA; Department of Otolaryngology-Head and Neck Surgery, Case Western Reserve University School of Medicine, Cleveland, OH USA. Electronic address: kna3@case.edu., Mellott JG; Department of Biomedical Sciences, Northeast Ohio Medical University, Rootstown, OH USA; University Hospitals | NEOMED Hearing Research Center, Northeast Ohio Medical University, Rootstown, OH USA. Electronic address: jmellott@neomed.edu.
Source: Hearing research [Hear Res] 2026 Sep; Vol. 479, pp. 109713. Date of Electronic Publication: 2026 Jun 25.
Publication Type: Journal Article; Research Support, N.I.H., Extramural
Language: English
Journal Info: Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 7900445 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-5891 (Electronic) Linking ISSN: 03785955 NLM ISO Abbreviation: Hear Res Subsets: MEDLINE
Imprint Name(s): Original Publication: Amsterdam, Elsevier/North-Holland Biomedical Press.
MeSH Terms: RNA, Messenger*/metabolism , RNA, Messenger*/genetics , Auditory Cortex*/metabolism , Cochlear Nucleus*/metabolism , Auditory Pathways*/metabolism , Membrane Proteins*/genetics , Membrane Proteins*/metabolism, Inferior Colliculi/metabolism ; Geniculate Bodies/metabolism ; Usher Syndromes/genetics ; Usher Syndromes/metabolism ; Usher Syndromes/physiopathology ; Animals ; Rats, Inbred F344 ; Rats, Inbred BN ; In Situ Hybridization, Fluorescence ; Male
Abstract: Hereditary hearing loss is frequently linked to hair cell defects, even when the associated genes are expressed in the inner ear and other tissues including the brain. An example of this scenario is the clain-1 gene, associated with progressive loss of hearing in Usher syndrome type IIIA (USH3A). While mouse studies demonstrate that clarin-1 is an essential hair cell gene and early cochlear gene therapy shows promise, a comprehensive understanding of clarin-1's role in the auditory system may lead to better therapeutic and rehabilitation strategies for USH3A. To target future research effectively, we first mapped clarin-1 expression across central auditory system (CAS). We used small molecule fluorescent in situ hybridization (smFISH) to map clarin-1 mRNA expression in the central auditory nuclei of the rat brain. Our data reveal two key findings: clarin-1 mRNA is expressed in the cochlear nucleus (CN), inferior colliculus (IC), medial geniculate body (MG), and auditory cortex (AC). Also, the proportion of cells expressing clarin-1 varies across these regions, with the highest concentration in the IC. These results merit investigation of the role of clarin-1 in the nuclei of the CAS.
(Copyright © 2026 The Author(s). Published by Elsevier B.V. All rights reserved.)
Competing Interests: Declaration of competing interest The authors declare that they have no conflict of interest.
Contributed Indexing: Keywords: Central auditory system; Clarin-1; Clarin-1 mRNA; smFISH
Substance Nomenclature: 0 (RNA, Messenger)
0 (Membrane Proteins)
Entry Date(s): Date Created: 20260625 Date Completed: 20260729 Latest Revision: 20260807
Update Code: 20260808
DOI: 10.1016/j.heares.2026.109713
PMID: 42349043
Database: MEDLINE
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  Data: Clarin-1 mRNA expression in the central auditory system of Fischer Brown Norway rats.
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  Data: Hereditary hearing loss is frequently linked to hair cell defects, even when the associated genes are expressed in the inner ear and other tissues including the brain. An example of this scenario is the clain-1 gene, associated with progressive loss of hearing in Usher syndrome type IIIA (USH3A). While mouse studies demonstrate that clarin-1 is an essential hair cell gene and early cochlear gene therapy shows promise, a comprehensive understanding of clarin-1's role in the auditory system may lead to better therapeutic and rehabilitation strategies for USH3A. To target future research effectively, we first mapped clarin-1 expression across central auditory system (CAS). We used small molecule fluorescent in situ hybridization (smFISH) to map clarin-1 mRNA expression in the central auditory nuclei of the rat brain. Our data reveal two key findings: clarin-1 mRNA is expressed in the cochlear nucleus (CN), inferior colliculus (IC), medial geniculate body (MG), and auditory cortex (AC). Also, the proportion of cells expressing clarin-1 varies across these regions, with the highest concentration in the IC. These results merit investigation of the role of clarin-1 in the nuclei of the CAS.<br /> (Copyright © 2026 The Author(s). Published by Elsevier B.V. All rights reserved.)
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  Data: Declaration of competing interest The authors declare that they have no conflict of interest.
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