Academic Journal
Bacillus subtilis expressing double-stranded RNA induced RNA interference in Tribolium castaneum.
| Title: | Bacillus subtilis expressing double-stranded RNA induced RNA interference in Tribolium castaneum. |
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| Authors: | He L; School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.; Shanghai Yangtze River Delta Eco-Environmental Change and Management Observation and Research Station (Shanghai Urban Ecosystem Research Station), Ministry of Science and Technology, National Forestry and Grassland Administration, Shanghai, China., Zhou Y; School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.; Shanghai Yangtze River Delta Eco-Environmental Change and Management Observation and Research Station (Shanghai Urban Ecosystem Research Station), Ministry of Science and Technology, National Forestry and Grassland Administration, Shanghai, China., Xie J; School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.; Shanghai Yangtze River Delta Eco-Environmental Change and Management Observation and Research Station (Shanghai Urban Ecosystem Research Station), Ministry of Science and Technology, National Forestry and Grassland Administration, Shanghai, China., Gu Y; Syngenta Jealott's Hill International Research Centre, Berkshire, UK., Huang Y; School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.; Shanghai Yangtze River Delta Eco-Environmental Change and Management Observation and Research Station (Shanghai Urban Ecosystem Research Station), Ministry of Science and Technology, National Forestry and Grassland Administration, Shanghai, China., Tang X; School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.; Shanghai Yangtze River Delta Eco-Environmental Change and Management Observation and Research Station (Shanghai Urban Ecosystem Research Station), Ministry of Science and Technology, National Forestry and Grassland Administration, Shanghai, China. |
| Source: | Pest management science [Pest Manag Sci] 2026 Oct; Vol. 82 (10), pp. 10073-10080. Date of Electronic Publication: 2026 Jun 24. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Published for SCI by Wiley Country of Publication: England NLM ID: 100898744 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1526-4998 (Electronic) Linking ISSN: 1526498X NLM ISO Abbreviation: Pest Manag Sci Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: West Sussex, UK : Published for SCI by Wiley, c2000- |
| MeSH Terms: | Bacillus subtilis*/genetics , Bacillus subtilis*/metabolism , RNA, Double-Stranded*/genetics , RNA, Double-Stranded*/metabolism , Tribolium*/genetics , Tribolium*/growth & development , Pest Control, Biological*/methods , RNA Interference*, Larva/growth & development ; Larva/genetics ; Animals |
| Abstract: | Background: RNA interference represents a promising and eco-friendly approach for pest management. As the core component of RNA biopesticides, the synthesis of double-stranded RNA (dsRNA) is the cornerstone for the further development and practical application of these biopesticides. Bacillus subtilis, with favorable characteristics (e.g., excellent safety, strong heterologous expression capability, and easy industrial fermentation), is an attractive dsRNA bio-factory for large-scale production of RNA biopesticides. Results: An RNase-III-deficient B. subtilis strain was successfully constructed. Among the promoters screened from genes with high expression levels at each growth stage, pcotY, pcgeA, and phag exhibited high potential in driving dsRNA expression. Further optimization of the promoter phag significantly improved the yield of dsRNA. Using the mutant B. subtilis strain as the host, the optimized phag derivative phag-1 was utilized to express dsRNA targeting TcSpt5 and TcPolr2A of Tribolium castaneum. Larvae fed on an artificial diet containing these recombinant strains displayed significant reductions in survival and pupation rate, accompanied by reduced target gene expression. Conclusion: Collectively, B. subtilis serves as an efficient dsRNA production platform that can be employed to control pests. © 2026 Society of Chemical Industry. (© 2026 Society of Chemical Industry.) |
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| Grant Information: | the National Key Research and Development Program of China (2023YFD1700700); the 2025Yangtze River Delta Science and Technology Innovation Community Joint Research (Basic Research) Project (2025CSJZN01300); the Syngenta PhD Scholarship; the Shanghai Agricultural Science and Technology Innovation Project (K2025007) |
| Contributed Indexing: | Keywords: Bacillus subtilis; RNAi; Tribolium castaneum; dsRNA; promoter |
| Substance Nomenclature: | 0 (RNA, Double-Stranded) |
| Entry Date(s): | Date Created: 20260624 Date Completed: 20260911 Latest Revision: 20260913 |
| Update Code: | 20260913 |
| PubMed Central ID: | PMC13569281 |
| DOI: | 10.1002/ps.71045 |
| PMID: | 42340137 |
| Database: | MEDLINE |
| ISSN: | 1526-4998 |
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| DOI: | 10.1002/ps.71045 |