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Optimizing phage elution for biopanning of plastic materials.

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Title: Optimizing phage elution for biopanning of plastic materials.
Authors: Lyngsø NM; Interdisciplinary Nanoscience Center, Aarhus University, Gustav Wieds Vej 14, Aarhus, 8000, Denmark., Otzen DE; Interdisciplinary Nanoscience Center, Aarhus University, Gustav Wieds Vej 14, Aarhus, 8000, Denmark. Electronic address: dao@inano.au.dk.
Source: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2026 Sep 03; Vol. 829, pp. 154187. Date of Electronic Publication: 2026 Jun 22.
Publication Type: Journal Article
Language: English
Journal Info: Publisher: Elsevier Country of Publication: United States NLM ID: 0372516 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2104 (Electronic) Linking ISSN: 0006291X NLM ISO Abbreviation: Biochem Biophys Res Commun Subsets: MEDLINE
Imprint Name(s): Publication: <2002- >: San Diego, CA : Elsevier
Original Publication: New York, Academic Press.
MeSH Terms: Plastics*/chemistry , Peptides*/chemistry , Peptides*/isolation & purification , Peptide Library* , Bacteriophages*, Surface-Active Agents/chemistry ; Ethanol/chemistry ; Hydrogen-Ion Concentration
Abstract: Biopanning of phage-displayed peptides is a useful method for peptide probe discovery and has been applied to multiple plastic targets. The most widely used elution method on plastic targets, acidic glycine buffer, is well-established as a general binding disrupter for protein targets, but a comparative study of elution buffers for plastic targets has never been published. Here we demonstrate how elution from plastics with mild surfactant C12E8 or ethanol increases phage yield up to 1000-fold compared to the commonly used acidic elution while also yielding more diverse phage populations. This implies that the classical acidic elution method fails to recover a substantial fraction of plastic binding phage clones. The alternative elution methods described here should therefore be considered when applying biopanning to non-protein targets, where acidic elution is unlikely to disrupt binding through pH-induced conformational changes.
(Copyright © 2026 The Authors. Published by Elsevier Inc. All rights reserved.)
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Comments: Erratum in: Biochem Biophys Res Commun. 2026 Aug 10:154401. doi: 10.1016/j.bbrc.2026.154401.. (PMID: 42575822)
Substance Nomenclature: 0 (Plastics)
0 (Peptide Library)
0 (Surface-Active Agents)
3K9958V90M (Ethanol)
0 (Peptides)
Entry Date(s): Date Created: 20260623 Date Completed: 20260716 Latest Revision: 20260810
Update Code: 20260811
DOI: 10.1016/j.bbrc.2026.154187
PMID: 42335658
Database: MEDLINE
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  Data: Optimizing phage elution for biopanning of plastic materials.
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  Data: Biopanning of phage-displayed peptides is a useful method for peptide probe discovery and has been applied to multiple plastic targets. The most widely used elution method on plastic targets, acidic glycine buffer, is well-established as a general binding disrupter for protein targets, but a comparative study of elution buffers for plastic targets has never been published. Here we demonstrate how elution from plastics with mild surfactant C12E8 or ethanol increases phage yield up to 1000-fold compared to the commonly used acidic elution while also yielding more diverse phage populations. This implies that the classical acidic elution method fails to recover a substantial fraction of plastic binding phage clones. The alternative elution methods described here should therefore be considered when applying biopanning to non-protein targets, where acidic elution is unlikely to disrupt binding through pH-induced conformational changes.&lt;br /&gt; (Copyright &#169; 2026 The Authors. Published by Elsevier Inc. All rights reserved.)
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  Data: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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        Value: 10.1016/j.bbrc.2026.154187
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        Text: English
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        StartPage: 154187
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        Type: general
      – SubjectFull: Ethanol chemistry
        Type: general
      – SubjectFull: Hydrogen-Ion Concentration
        Type: general
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