Academic Journal
The genomic resource of Lysinibacillus fusiformis KBD-5, a biocontrol agent with antifungal activity against Botrytis cinerea.
| Τίτλος: | The genomic resource of Lysinibacillus fusiformis KBD-5, a biocontrol agent with antifungal activity against Botrytis cinerea. |
|---|---|
| Συγγραφείς: | Gao Y; MARA Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co- construction by Ministry and Province), Yangtze University, Jingzhou, 434025, China., Zhu Q; Anhui Wannan Tobacco Leaf Co., Ltd, Xuancheng, 242000, China., Yuan L; Key Laboratory of Tobacco Pest Monitoring Controlling and Integrated Management, State Tobacco Monopoly Administration (China National Tobacco Corporation), Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, 266101, China., Jiao Y; Key Laboratory of Tobacco Pest Monitoring Controlling and Integrated Management, State Tobacco Monopoly Administration (China National Tobacco Corporation), Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, 266101, China., Wang Y; Key Laboratory of Tobacco Pest Monitoring Controlling and Integrated Management, State Tobacco Monopoly Administration (China National Tobacco Corporation), Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, 266101, China., Pei Z; Anhui Wannan Tobacco Leaf Co., Ltd, Xuancheng, 242000, China., Shen L; Key Laboratory of Tobacco Pest Monitoring Controlling and Integrated Management, State Tobacco Monopoly Administration (China National Tobacco Corporation), Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, 266101, China., Li Y; Key Laboratory of Tobacco Pest Monitoring Controlling and Integrated Management, State Tobacco Monopoly Administration (China National Tobacco Corporation), Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, 266101, China., Shen S; Anhui Wannan Tobacco Leaf Co., Ltd, Xuancheng, 242000, China., Zhang S; MARA Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co- construction by Ministry and Province), Yangtze University, Jingzhou, 434025, China., Yang J; Key Laboratory of Tobacco Pest Monitoring Controlling and Integrated Management, State Tobacco Monopoly Administration (China National Tobacco Corporation), Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, 266101, China., Lv B; Key Laboratory of Tobacco Pest Monitoring Controlling and Integrated Management, State Tobacco Monopoly Administration (China National Tobacco Corporation), Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, 266101, China. lvbinna@caas.cn. |
| Πηγή: | BMC genomics [BMC Genomics] 2026 Jun 22; Vol. 27 (1). Date of Electronic Publication: 2026 Jun 22. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: BioMed Central Country of Publication: England NLM ID: 100965258 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2164 (Electronic) Linking ISSN: 14712164 NLM ISO Abbreviation: BMC Genomics Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: London : BioMed Central, [2000- |
| Ιατρικοί όροι (MeSH): | Botrytis*/drug effects , Botrytis*/growth & development , Antifungal Agents*/pharmacology , Antifungal Agents*/metabolism , Bacillaceae*/genetics , Bacillaceae*/metabolism , Genome, Bacterial* , Biological Control Agents* , Genomics*, Multigene Family ; Whole Genome Sequencing |
| Περίληψη: | Lysinibacillus fusiformis strain KBD-5, previously known for its antiviral activity against Tobacco mosaic virus, was investigated for its biocontrol potential against the fungal pathogen Botrytis cinerea. In plate assays, conducted with three independent biological replicates and incubated at 28 °C for 5 days, KBD-5 significantly inhibited the mycelial growth of B. cinerea by 76.42%. Whole-genome sequencing revealed a 4.69 Mb genome with a GC content of 37.28%, encoding 4719 proteins. Bioinformatics analysis identified genes involved in antimicrobial functions, including 195 carbohydrate-active enzymes (potentially aiding in fungal cell wall degradation) and 8 gene clusters for secondary metabolite synthesis (e.g., T3PKS with 30% similarity to bacillibactin biosynthetic clusters and NRPS), indicating the production of antifungal metabolites like bacillibactin-like polyketides. The strain also showed a high safety profile with no significant virulence or drug resistance risks. These findings indicate that genomic analysis of KBD-5 reveals the potential for multiple biocontrol mechanisms, supporting its potential development as a biocontrol agent. The draft genome sequence is available under NCBI accession PRJNA1335659. (© 2026. The Author(s).) |
| Competing Interests: | Declarations. Ethics approval and consent to participate: Not applicable. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. |
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| Grant Information: | WNYY-0563-202502013 Tobacco Green Pest Control Project of Anhui Wannan Tobacco Leaf Co., Ltd.; 32502575 National Natural Science Foundation of China; ZR2025QC247 Shandong Provincial Natural Science Foundation; ASTIP-TRIC-QH-2025B02 The Agricultural Science and Technology Innovation Program |
| Contributed Indexing: | Keywords: Lysinibacillus fusiformis; Biological control; Carbohydrate-active enzymes; Secondary metabolites; Whole-genome sequencing |
| Substance Nomenclature: | 0 (Antifungal Agents) 0 (Biological Control Agents) |
| SCR Organism: | Botrytis cinerea |
| Entry Date(s): | Date Created: 20260623 Date Completed: 20260903 Latest Revision: 20260903 |
| Update Code: | 20260903 |
| DOI: | 10.1186/s12864-026-13100-3 |
| PMID: | 42332540 |
| Βάση Δεδομένων: | MEDLINE |
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