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The genomic resource of Lysinibacillus fusiformis KBD-5, a biocontrol agent with antifungal activity against Botrytis cinerea.

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Τίτλος: The genomic resource of Lysinibacillus fusiformis KBD-5, a biocontrol agent with antifungal activity against Botrytis cinerea.
Συγγραφείς: Gao Y; MARA Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co- construction by Ministry and Province), Yangtze University, Jingzhou, 434025, China., Zhu Q; Anhui Wannan Tobacco Leaf Co., Ltd, Xuancheng, 242000, China., Yuan L; Key Laboratory of Tobacco Pest Monitoring Controlling and Integrated Management, State Tobacco Monopoly Administration (China National Tobacco Corporation), Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, 266101, China., Jiao Y; Key Laboratory of Tobacco Pest Monitoring Controlling and Integrated Management, State Tobacco Monopoly Administration (China National Tobacco Corporation), Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, 266101, China., Wang Y; Key Laboratory of Tobacco Pest Monitoring Controlling and Integrated Management, State Tobacco Monopoly Administration (China National Tobacco Corporation), Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, 266101, China., Pei Z; Anhui Wannan Tobacco Leaf Co., Ltd, Xuancheng, 242000, China., Shen L; Key Laboratory of Tobacco Pest Monitoring Controlling and Integrated Management, State Tobacco Monopoly Administration (China National Tobacco Corporation), Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, 266101, China., Li Y; Key Laboratory of Tobacco Pest Monitoring Controlling and Integrated Management, State Tobacco Monopoly Administration (China National Tobacco Corporation), Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, 266101, China., Shen S; Anhui Wannan Tobacco Leaf Co., Ltd, Xuancheng, 242000, China., Zhang S; MARA Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co- construction by Ministry and Province), Yangtze University, Jingzhou, 434025, China., Yang J; Key Laboratory of Tobacco Pest Monitoring Controlling and Integrated Management, State Tobacco Monopoly Administration (China National Tobacco Corporation), Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, 266101, China., Lv B; Key Laboratory of Tobacco Pest Monitoring Controlling and Integrated Management, State Tobacco Monopoly Administration (China National Tobacco Corporation), Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, 266101, China. lvbinna@caas.cn.
Πηγή: BMC genomics [BMC Genomics] 2026 Jun 22; Vol. 27 (1). Date of Electronic Publication: 2026 Jun 22.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: BioMed Central Country of Publication: England NLM ID: 100965258 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2164 (Electronic) Linking ISSN: 14712164 NLM ISO Abbreviation: BMC Genomics Subsets: MEDLINE
Imprint Name(s): Original Publication: London : BioMed Central, [2000-
Ιατρικοί όροι (MeSH): Botrytis*/drug effects , Botrytis*/growth & development , Antifungal Agents*/pharmacology , Antifungal Agents*/metabolism , Bacillaceae*/genetics , Bacillaceae*/metabolism , Genome, Bacterial* , Biological Control Agents* , Genomics*, Multigene Family ; Whole Genome Sequencing
Περίληψη: Lysinibacillus fusiformis strain KBD-5, previously known for its antiviral activity against Tobacco mosaic virus, was investigated for its biocontrol potential against the fungal pathogen Botrytis cinerea. In plate assays, conducted with three independent biological replicates and incubated at 28 °C for 5 days, KBD-5 significantly inhibited the mycelial growth of B. cinerea by 76.42%. Whole-genome sequencing revealed a 4.69 Mb genome with a GC content of 37.28%, encoding 4719 proteins. Bioinformatics analysis identified genes involved in antimicrobial functions, including 195 carbohydrate-active enzymes (potentially aiding in fungal cell wall degradation) and 8 gene clusters for secondary metabolite synthesis (e.g., T3PKS with 30% similarity to bacillibactin biosynthetic clusters and NRPS), indicating the production of antifungal metabolites like bacillibactin-like polyketides. The strain also showed a high safety profile with no significant virulence or drug resistance risks. These findings indicate that genomic analysis of KBD-5 reveals the potential for multiple biocontrol mechanisms, supporting its potential development as a biocontrol agent. The draft genome sequence is available under NCBI accession PRJNA1335659.
(© 2026. The Author(s).)
Competing Interests: Declarations. Ethics approval and consent to participate: Not applicable. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests.
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Vitorino LC, da Silva EJ, Oliveira MS, et al. Effect of a Bacillus velezensis and Lysinibacillus fusiformis-based biofertilizer on phosphorus acquisition and grain yield of soybean [J]. Front Plant Sci. 2024;15:1433828. (PMID: 10.3389/fpls.2024.14338283924681011378753)
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Grant Information: WNYY-0563-202502013 Tobacco Green Pest Control Project of Anhui Wannan Tobacco Leaf Co., Ltd.; 32502575 National Natural Science Foundation of China; ZR2025QC247 Shandong Provincial Natural Science Foundation; ASTIP-TRIC-QH-2025B02 The Agricultural Science and Technology Innovation Program
Contributed Indexing: Keywords: Lysinibacillus fusiformis; Biological control; Carbohydrate-active enzymes; Secondary metabolites; Whole-genome sequencing
Substance Nomenclature: 0 (Antifungal Agents)
0 (Biological Control Agents)
SCR Organism: Botrytis cinerea
Entry Date(s): Date Created: 20260623 Date Completed: 20260903 Latest Revision: 20260903
Update Code: 20260903
DOI: 10.1186/s12864-026-13100-3
PMID: 42332540
Βάση Δεδομένων: MEDLINE
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  Label: Title
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  Data: The genomic resource of Lysinibacillus fusiformis KBD-5, a biocontrol agent with antifungal activity against Botrytis cinerea.
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  Data: <searchLink fieldCode="AU" term="%22Gao+Y%22">Gao Y</searchLink>; MARA Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co- construction by Ministry and Province), Yangtze University, Jingzhou, 434025, China.<br /><searchLink fieldCode="AU" term="%22Zhu+Q%22">Zhu Q</searchLink>; Anhui Wannan Tobacco Leaf Co., Ltd, Xuancheng, 242000, China.<br /><searchLink fieldCode="AU" term="%22Yuan+L%22">Yuan L</searchLink>; Key Laboratory of Tobacco Pest Monitoring Controlling and Integrated Management, State Tobacco Monopoly Administration (China National Tobacco Corporation), Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, 266101, China.<br /><searchLink fieldCode="AU" term="%22Jiao+Y%22">Jiao Y</searchLink>; Key Laboratory of Tobacco Pest Monitoring Controlling and Integrated Management, State Tobacco Monopoly Administration (China National Tobacco Corporation), Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, 266101, China.<br /><searchLink fieldCode="AU" term="%22Wang+Y%22">Wang Y</searchLink>; Key Laboratory of Tobacco Pest Monitoring Controlling and Integrated Management, State Tobacco Monopoly Administration (China National Tobacco Corporation), Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, 266101, China.<br /><searchLink fieldCode="AU" term="%22Pei+Z%22">Pei Z</searchLink>; Anhui Wannan Tobacco Leaf Co., Ltd, Xuancheng, 242000, China.<br /><searchLink fieldCode="AU" term="%22Shen+L%22">Shen L</searchLink>; Key Laboratory of Tobacco Pest Monitoring Controlling and Integrated Management, State Tobacco Monopoly Administration (China National Tobacco Corporation), Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, 266101, China.<br /><searchLink fieldCode="AU" term="%22Li+Y%22">Li Y</searchLink>; Key Laboratory of Tobacco Pest Monitoring Controlling and Integrated Management, State Tobacco Monopoly Administration (China National Tobacco Corporation), Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, 266101, China.<br /><searchLink fieldCode="AU" term="%22Shen+S%22">Shen S</searchLink>; Anhui Wannan Tobacco Leaf Co., Ltd, Xuancheng, 242000, China.<br /><searchLink fieldCode="AU" term="%22Zhang+S%22">Zhang S</searchLink>; MARA Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co- construction by Ministry and Province), Yangtze University, Jingzhou, 434025, China.<br /><searchLink fieldCode="AU" term="%22Yang+J%22">Yang J</searchLink>; Key Laboratory of Tobacco Pest Monitoring Controlling and Integrated Management, State Tobacco Monopoly Administration (China National Tobacco Corporation), Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, 266101, China.<br /><searchLink fieldCode="AU" term="%22Lv+B%22">Lv B</searchLink>; Key Laboratory of Tobacco Pest Monitoring Controlling and Integrated Management, State Tobacco Monopoly Administration (China National Tobacco Corporation), Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, 266101, China. lvbinna@caas.cn.
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  Data: <searchLink fieldCode="JN" term="%22100965258%22">BMC genomics</searchLink> [BMC Genomics] 2026 Jun 22; Vol. 27 (1). <i>Date of Electronic Publication: </i>2026 Jun 22.
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  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22BioMed+Central%22">BioMed Central </searchLink><i>Country of Publication: </i>England <i>NLM ID: </i>100965258 <i>Publication Model: </i>Electronic <i>Cited Medium: </i>Internet <i>ISSN: </i>1471-2164 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2214712164%22">14712164 </searchLink><i>NLM ISO Abbreviation: </i>BMC Genomics <i>Subsets: </i>MEDLINE
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  Data: <searchLink fieldCode="MM" term="%22Botrytis%22">Botrytis*</searchLink>/<searchLink fieldCode="MM" term="%22Botrytis+drug+effects%22">drug effects</searchLink> <br /><searchLink fieldCode="MM" term="%22Botrytis%22">Botrytis*</searchLink>/<searchLink fieldCode="MM" term="%22Botrytis+growth+%26+development%22">growth & development</searchLink> <br /><searchLink fieldCode="MM" term="%22Antifungal+Agents%22">Antifungal Agents*</searchLink>/<searchLink fieldCode="MM" term="%22Antifungal+Agents+pharmacology%22">pharmacology</searchLink> <br /><searchLink fieldCode="MM" term="%22Antifungal+Agents%22">Antifungal Agents*</searchLink>/<searchLink fieldCode="MM" term="%22Antifungal+Agents+metabolism%22">metabolism</searchLink> <br /><searchLink fieldCode="MM" term="%22Bacillaceae%22">Bacillaceae*</searchLink>/<searchLink fieldCode="MM" term="%22Bacillaceae+genetics%22">genetics</searchLink> <br /><searchLink fieldCode="MM" term="%22Bacillaceae%22">Bacillaceae*</searchLink>/<searchLink fieldCode="MM" term="%22Bacillaceae+metabolism%22">metabolism</searchLink> <br /><searchLink fieldCode="MM" term="%22Genome%2C+Bacterial%22">Genome, Bacterial*</searchLink> <br /><searchLink fieldCode="MM" term="%22Biological+Control+Agents%22">Biological Control Agents*</searchLink> <br /><searchLink fieldCode="MM" term="%22Genomics%22">Genomics*</searchLink><br /><searchLink fieldCode="MH" term="%22Multigene+Family%22">Multigene Family</searchLink> ; <searchLink fieldCode="MH" term="%22Whole+Genome+Sequencing%22">Whole Genome Sequencing</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Lysinibacillus fusiformis strain KBD-5, previously known for its antiviral activity against Tobacco mosaic virus, was investigated for its biocontrol potential against the fungal pathogen Botrytis cinerea. In plate assays, conducted with three independent biological replicates and incubated at 28 °C for 5 days, KBD-5 significantly inhibited the mycelial growth of B. cinerea by 76.42%. Whole-genome sequencing revealed a 4.69 Mb genome with a GC content of 37.28%, encoding 4719 proteins. Bioinformatics analysis identified genes involved in antimicrobial functions, including 195 carbohydrate-active enzymes (potentially aiding in fungal cell wall degradation) and 8 gene clusters for secondary metabolite synthesis (e.g., T3PKS with 30% similarity to bacillibactin biosynthetic clusters and NRPS), indicating the production of antifungal metabolites like bacillibactin-like polyketides. The strain also showed a high safety profile with no significant virulence or drug resistance risks. These findings indicate that genomic analysis of KBD-5 reveals the potential for multiple biocontrol mechanisms, supporting its potential development as a biocontrol agent. The draft genome sequence is available under NCBI accession PRJNA1335659.<br /> (© 2026. The Author(s).)
– Name: Abstract
  Label: Competing Interests
  Group: Ab
  Data: Declarations. Ethics approval and consent to participate: Not applicable. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests.
– Name: Ref
  Label: References
  Group: RefInfo
  Data: Li J, Xiang C, Wang X, et al. Current situation and prospect of tomato industry during the 13th Five-Year Plan period [J]. Chin Veg. 2021;1(02):13–20.<br />Jing Z, Guo Z, Xu S. Screening and identification of Bacillus amyloliquefaciens HZ-6-3 and evaluation of its effect on controlling tomato gray mold [J]. Acta Pratacult Sin. 2020;29(02):31–41.<br />Zhang T, Gao X, Tian R, et al. Research progress on the control of tomato gray mold [J]. Hortic Seed. 2022;42(02):28–9.<br />Bu Y, Zhang S, Zhang X et al. Study on nano-selenium application of Lysinibacillus fusiformis KBD-5 [J]. J Gansu Agric Univ. 2025.<br />Huang H, Luo K. Research progress on the combination of Bacillus and fungicides for controlling plant diseases [J]. Microbiol. 2021;48(03):938–47.<br />Tuipulotu DE, Mathur A, Ngo C. Bacillus cereus: epidemiology, virulence factors, and host-pathogen interactions [J]. Trends Microbiol. 2021;29(5):458–71. (PMID: <searchLink fieldCode="PM" term="%2210%2E1016%2Fj%2Etim%2E2020%2E09%2E003%22">10.1016/j.tim.2020.09.003)</searchLink><br />Xie J, Chen M, Wang B, et al. Control effect of Bacillus velezensis EEAM 10B on peanut southern blight and analysis of whole genome sequencing [J]. Chin J Biol Control. 2025;41(02):347–61.<br />Li H, He M, Gao X, et al. Whole genome sequencing analysis of biocontrol bacterium Bacillus velezensis MC2-1 [J]. J South Agric. 2022;53(12):3420–32.<br />Cao X, Ye Y, Ma T, et al. Whole genome sequencing and analysis of biocontrol strain Bacillus velezensis HNU24 [J]. Chin J Trop Crops. 2023;44(05):905–13.<br />Rabbee MF, Ali MS, Choi J, et al. Bacillus velezensis: A Valuable Member of Bioactive Molecules within Plant Microbiomes [J]. Molecules. 2019;24(6):1046. (PMID: <searchLink fieldCode="PM" term="%2210%2E3390%2Fmolecules24061046308848576470737%22">10.3390/molecules24061046308848576470737)</searchLink><br />Liu Y, Chen M, Pang X. Isolation and identification of an endophytic bacterium Lysinibacillus fusiformis S-1 producing extracellular polysaccharides from yam [J]. Chin J Appl Environ Biol. 2014;20(03):382–8. (PMID: <searchLink fieldCode="PM" term="%2210%2E3724%2FSP%2EJ%2E1145%2E2014%2E09040%22">10.3724/SP.J.1145.2014.09040)</searchLink><br />Ishaq S, Belduz AO, Ceylan E, et al. Plant growth-promoting bacteria from Uzungöl forest stimulate rice growth via seed biopriming and root inoculation: isolation and functional characterization of potent PGPR strains from rhizosphere soils of different trees [J]. Front Plant Sci. 2025;16:1622951. (PMID: <searchLink fieldCode="PM" term="%2210%2E3389%2Ffpls%2E2025%2E16229514077706012328432%22">10.3389/fpls.2025.16229514077706012328432)</searchLink><br />Vitorino LC, da Silva EJ, Oliveira MS, et al. Effect of a Bacillus velezensis and Lysinibacillus fusiformis-based biofertilizer on phosphorus acquisition and grain yield of soybean [J]. Front Plant Sci. 2024;15:1433828. 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– Name: GrantInfo
  Label: Grant Information
  Group: Grant
  Data: WNYY-0563-202502013 Tobacco Green Pest Control Project of Anhui Wannan Tobacco Leaf Co., Ltd.; 32502575 National Natural Science Foundation of China; ZR2025QC247 Shandong Provincial Natural Science Foundation; ASTIP-TRIC-QH-2025B02 The Agricultural Science and Technology Innovation Program
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  Data: <i>Keywords: </i>Lysinibacillus fusiformis; Biological control; Carbohydrate-active enzymes; Secondary metabolites; Whole-genome sequencing
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  Data: Botrytis cinerea
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  Data: <i>Date Created: </i>20260623 <i>Date Completed: </i>20260903 <i>Latest Revision: </i>20260903
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PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=cmedm&AN=42332540
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      – SubjectFull: Whole Genome Sequencing
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      – SubjectFull: Botrytis drug effects
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              Text: 2026 Jun 22
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