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Reduction and resistance of vanadate by Lysinibacillus fusiformis isolated from vanadium contaminated groundwater.

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Τίτλος: Reduction and resistance of vanadate by Lysinibacillus fusiformis isolated from vanadium contaminated groundwater.
Συγγραφείς: Wang F; State Key Laboratory of Geomicrobiology and Environmental Changes, Frontiers Science Center for Deep-time Digital Earth, China University of Geosciences Beijing, Beijing 100083, PR China; MOE Key Laboratory of Groundwater Circulation and Environmental Evolution, School of Water Resources and Environment, China University of Geosciences Beijing, Beijing 100083, PR China., Wang S; State Key Laboratory of Geomicrobiology and Environmental Changes, Frontiers Science Center for Deep-time Digital Earth, China University of Geosciences Beijing, Beijing 100083, PR China; MOE Key Laboratory of Groundwater Circulation and Environmental Evolution, School of Water Resources and Environment, China University of Geosciences Beijing, Beijing 100083, PR China. Electronic address: sowang@cugb.edu.cn., Song C; State Key Laboratory of Geomicrobiology and Environmental Changes, Frontiers Science Center for Deep-time Digital Earth, China University of Geosciences Beijing, Beijing 100083, PR China; MOE Key Laboratory of Groundwater Circulation and Environmental Evolution, School of Water Resources and Environment, China University of Geosciences Beijing, Beijing 100083, PR China., Zhang B; State Key Laboratory of Geomicrobiology and Environmental Changes, Frontiers Science Center for Deep-time Digital Earth, China University of Geosciences Beijing, Beijing 100083, PR China; MOE Key Laboratory of Groundwater Circulation and Environmental Evolution, School of Water Resources and Environment, China University of Geosciences Beijing, Beijing 100083, PR China. Electronic address: baogangzhang@cugb.edu.cn.
Πηγή: Journal of hazardous materials [J Hazard Mater] 2026 Aug 01; Vol. 514, pp. 142745. Date of Electronic Publication: 2026 Jun 18.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 9422688 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3336 (Electronic) Linking ISSN: 03043894 NLM ISO Abbreviation: J Hazard Mater Subsets: MEDLINE
Imprint Name(s): Original Publication: Amsterdam : Elsevier
Ιατρικοί όροι (MeSH): Groundwater*/microbiology , Groundwater*/chemistry , Bacillaceae*/metabolism , Bacillaceae*/genetics , Bacillaceae*/isolation & purification , Water Pollutants, Chemical*/metabolism , Vanadates*/metabolism , Vanadium*/metabolism, RNA, Ribosomal, 16S/genetics ; Biodegradation, Environmental ; Phylogeny ; Oxidation-Reduction
Περίληψη: Vanadium is widely detected in groundwater and poses significant risks to human health at elevated concentrations. Microbial remediation offers a promising and effective approach for vanadium treatment. However, vanadate [V(V)]-reducing microorganisms have mainly been characterized using pure metal-reducing strains, leaving the tolerance and reduction potential of indigenous groundwater microorganisms unclear. In this study, an efficient V(V)-reducing bacterium was isolated from vanadium-contaminated groundwater using an enrichment culture method. The isolated strain was then identified as Lysinibacillus fusiformis CUGB1 by combining 16S rDNA phylogenetic analysis with its biological features. Whole-gene sequencing was further performed to characterize the function of this strain. Up to 89.6% V(V) was removed in 120 h, producing the amorphous tetravalent vanadium precipitates. Microbial transcriptomic analysis under 10 mg/L V(V) revealed that functional genes related to carbohydrate synthesis, peptidoglycan biosynthesis, and spore coat formation (e.g., rpmD, cotD, cotY_Z) were upregulated under vanadium stress, suggesting a strategy of sporulation to resist vanadium. The elevated levels of membrane interfacial electron transfer compounds (e.g., cytochrome c, nicotinamide adenine dinucleotide, glutathione, extracellular polymeric substances) along with transmembrane electron shuttles (e.g., riboflavin and quinones) promoted electron transfer, thereby supporting V(V) reduction. This species was widely distributed in natural environments on Earth, displaying great bioremediation potential. This study expanded the family of known V(V) reducing microorganisms and contributed to the sustainable bioremediation of vanadium-contaminated groundwater.
(Copyright © 2026 Elsevier B.V. All rights reserved.)
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Contributed Indexing: Keywords: Bioremediation; Electron transfer; Groundwater; Spore; Transcriptomic analysis
Substance Nomenclature: 0 (Water Pollutants, Chemical)
3WHH0066W5 (Vanadates)
00J9J9XKDE (Vanadium)
0 (RNA, Ribosomal, 16S)
Entry Date(s): Date Created: 20260620 Date Completed: 20260725 Latest Revision: 20260725
Update Code: 20260725
DOI: 10.1016/j.jhazmat.2026.142745
PMID: 42322837
Βάση Δεδομένων: MEDLINE
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  Data: Reduction and resistance of vanadate by Lysinibacillus fusiformis isolated from vanadium contaminated groundwater.
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  Data: <searchLink fieldCode="AU" term="%22Wang+F%22">Wang F</searchLink>; State Key Laboratory of Geomicrobiology and Environmental Changes, Frontiers Science Center for Deep-time Digital Earth, China University of Geosciences Beijing, Beijing 100083, PR China; MOE Key Laboratory of Groundwater Circulation and Environmental Evolution, School of Water Resources and Environment, China University of Geosciences Beijing, Beijing 100083, PR China.<br /><searchLink fieldCode="AU" term="%22Wang+S%22">Wang S</searchLink>; State Key Laboratory of Geomicrobiology and Environmental Changes, Frontiers Science Center for Deep-time Digital Earth, China University of Geosciences Beijing, Beijing 100083, PR China; MOE Key Laboratory of Groundwater Circulation and Environmental Evolution, School of Water Resources and Environment, China University of Geosciences Beijing, Beijing 100083, PR China. Electronic address: sowang@cugb.edu.cn.<br /><searchLink fieldCode="AU" term="%22Song+C%22">Song C</searchLink>; State Key Laboratory of Geomicrobiology and Environmental Changes, Frontiers Science Center for Deep-time Digital Earth, China University of Geosciences Beijing, Beijing 100083, PR China; MOE Key Laboratory of Groundwater Circulation and Environmental Evolution, School of Water Resources and Environment, China University of Geosciences Beijing, Beijing 100083, PR China.<br /><searchLink fieldCode="AU" term="%22Zhang+B%22">Zhang B</searchLink>; State Key Laboratory of Geomicrobiology and Environmental Changes, Frontiers Science Center for Deep-time Digital Earth, China University of Geosciences Beijing, Beijing 100083, PR China; MOE Key Laboratory of Groundwater Circulation and Environmental Evolution, School of Water Resources and Environment, China University of Geosciences Beijing, Beijing 100083, PR China. Electronic address: baogangzhang@cugb.edu.cn.
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  Data: <i>Original Publication</i>: Amsterdam : Elsevier
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  Data: <searchLink fieldCode="MM" term="%22Groundwater%22">Groundwater*</searchLink>/<searchLink fieldCode="MM" term="%22Groundwater+microbiology%22">microbiology</searchLink> <br /><searchLink fieldCode="MM" term="%22Groundwater%22">Groundwater*</searchLink>/<searchLink fieldCode="MM" term="%22Groundwater+chemistry%22">chemistry</searchLink> <br /><searchLink fieldCode="MM" term="%22Bacillaceae%22">Bacillaceae*</searchLink>/<searchLink fieldCode="MM" term="%22Bacillaceae+metabolism%22">metabolism</searchLink> <br /><searchLink fieldCode="MM" term="%22Bacillaceae%22">Bacillaceae*</searchLink>/<searchLink fieldCode="MM" term="%22Bacillaceae+genetics%22">genetics</searchLink> <br /><searchLink fieldCode="MM" term="%22Bacillaceae%22">Bacillaceae*</searchLink>/<searchLink fieldCode="MM" term="%22Bacillaceae+isolation+%26+purification%22">isolation & purification</searchLink> <br /><searchLink fieldCode="MM" term="%22Water+Pollutants%2C+Chemical%22">Water Pollutants, Chemical*</searchLink>/<searchLink fieldCode="MM" term="%22Water+Pollutants%2C+Chemical+metabolism%22">metabolism</searchLink> <br /><searchLink fieldCode="MM" term="%22Vanadates%22">Vanadates*</searchLink>/<searchLink fieldCode="MM" term="%22Vanadates+metabolism%22">metabolism</searchLink> <br /><searchLink fieldCode="MM" term="%22Vanadium%22">Vanadium*</searchLink>/<searchLink fieldCode="MM" term="%22Vanadium+metabolism%22">metabolism</searchLink><br /><searchLink fieldCode="MH" term="%22RNA%2C+Ribosomal%2C+16S%22">RNA, Ribosomal, 16S</searchLink>/<searchLink fieldCode="MH" term="%22RNA%2C+Ribosomal%2C+16S+genetics%22">genetics</searchLink> ; <searchLink fieldCode="MH" term="%22Biodegradation%2C+Environmental%22">Biodegradation, Environmental</searchLink> ; <searchLink fieldCode="MH" term="%22Phylogeny%22">Phylogeny</searchLink> ; <searchLink fieldCode="MH" term="%22Oxidation-Reduction%22">Oxidation-Reduction</searchLink>
– Name: Abstract
  Label: Abstract
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  Data: Vanadium is widely detected in groundwater and poses significant risks to human health at elevated concentrations. Microbial remediation offers a promising and effective approach for vanadium treatment. However, vanadate [V(V)]-reducing microorganisms have mainly been characterized using pure metal-reducing strains, leaving the tolerance and reduction potential of indigenous groundwater microorganisms unclear. In this study, an efficient V(V)-reducing bacterium was isolated from vanadium-contaminated groundwater using an enrichment culture method. The isolated strain was then identified as Lysinibacillus fusiformis CUGB1 by combining 16S rDNA phylogenetic analysis with its biological features. Whole-gene sequencing was further performed to characterize the function of this strain. Up to 89.6% V(V) was removed in 120 h, producing the amorphous tetravalent vanadium precipitates. Microbial transcriptomic analysis under 10 mg/L V(V) revealed that functional genes related to carbohydrate synthesis, peptidoglycan biosynthesis, and spore coat formation (e.g., rpmD, cotD, cotY_Z) were upregulated under vanadium stress, suggesting a strategy of sporulation to resist vanadium. The elevated levels of membrane interfacial electron transfer compounds (e.g., cytochrome c, nicotinamide adenine dinucleotide, glutathione, extracellular polymeric substances) along with transmembrane electron shuttles (e.g., riboflavin and quinones) promoted electron transfer, thereby supporting V(V) reduction. This species was widely distributed in natural environments on Earth, displaying great bioremediation potential. This study expanded the family of known V(V) reducing microorganisms and contributed to the sustainable bioremediation of vanadium-contaminated groundwater.<br /> (Copyright © 2026 Elsevier B.V. All rights reserved.)
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  Data: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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  Data: <i>Keywords: </i>Bioremediation; Electron transfer; Groundwater; Spore; Transcriptomic analysis
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RecordInfo BibRecord:
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        Value: 10.1016/j.jhazmat.2026.142745
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        StartPage: 142745
    Subjects:
      – SubjectFull: RNA, Ribosomal, 16S genetics
        Type: general
      – SubjectFull: Biodegradation, Environmental
        Type: general
      – SubjectFull: Phylogeny
        Type: general
      – SubjectFull: Oxidation-Reduction
        Type: general
      – SubjectFull: Groundwater microbiology
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        Type: general
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        Type: general
      – SubjectFull: Water Pollutants, Chemical metabolism
        Type: general
      – SubjectFull: Vanadates metabolism
        Type: general
      – SubjectFull: Vanadium metabolism
        Type: general
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      – TitleFull: Reduction and resistance of vanadate by Lysinibacillus fusiformis isolated from vanadium contaminated groundwater.
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              Text: 2026 Aug 01
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