Academic Journal
Headgroup and acyl composition determine lipid recovery from silica in preparative HPTLC workflows.
| Τίτλος: | Headgroup and acyl composition determine lipid recovery from silica in preparative HPTLC workflows. |
|---|---|
| Συγγραφείς: | Schubarth JW; Leipzig University, Medical Department, Institute for Medical Physics and Biophysics, Härtelstr. 16/18, D-04107 Leipzig, Germany., Koller H; Leipzig University, Faculty of Chemistry, Institute of Analytical Chemistry, Mass Spectrometry Research Group, Linnéstraße 3, D-04103 Leipzig, Germany., Wiesner C; Leipzig University, Faculty of Chemistry, Institute of Analytical Chemistry, Mass Spectrometry Research Group, Linnéstraße 3, D-04103 Leipzig, Germany., Schiller J; Leipzig University, Medical Department, Institute for Medical Physics and Biophysics, Härtelstr. 16/18, D-04107 Leipzig, Germany. Electronic address: juergen.schiller@medizin.uni-leipzig.de. |
| Πηγή: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [J Chromatogr B Analyt Technol Biomed Life Sci] 2026 Sep 01; Vol. 1281, pp. 125191. Date of Electronic Publication: 2026 Jun 15. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101139554 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-376X (Electronic) Linking ISSN: 15700232 NLM ISO Abbreviation: J Chromatogr B Analyt Technol Biomed Life Sci Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Amsterdam ; New York : Elsevier, c2002- |
| Ιατρικοί όροι (MeSH): | Silicon Dioxide*/chemistry , Lipids*/isolation & purification , Lipids*/chemistry , Lipids*/analysis , Phospholipids*/chemistry , Phospholipids*/isolation & purification , Phospholipids*/analysis, Chromatography, Thin Layer/methods ; Chromatography, High Pressure Liquid |
| Περίληψη: | Thin-layer chromatography (TLC) is a widely used, cost-effective and convenient method for separating moderately complex mixtures. TLC is well suited for small, apolar compounds such as lipids and offers several advantages over high-performance liquid chromatography (HPLC). In contrast to HPLC, which commonly employs reversed-phase materials, TLC of (phospho)lipids traditionally relies on normal-phase separation using unmodified silica gel. Although TLC is well established for the analysis of (phospho)lipids, in particular when coupled to mass spectrometry (MS), details of the interactions between lipids and the stationary phase are still not completely understood. In this study, we investigate the extent to which the headgroup, the chain length and the number of double bonds of a dedicated phospholipid influences its extractability from the developed TLC plate. The results indicate that lipid recovery from HPTLC silica is influenced mainly by headgroup charge, while fatty acyl chain length and the degree of unsaturation contribute to a lesser extent. The impact of these effects on quantitative data analysis is discussed. (Copyright © 2026 The Authors. Published by Elsevier B.V. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Glycero-(phospho)-lipids; HPTLC; Lipids; Preparative TLC; TLC; TLC/MS coupling |
| Substance Nomenclature: | 7631-86-9 (Silicon Dioxide) 0 (Lipids) 0 (Phospholipids) |
| Entry Date(s): | Date Created: 20260618 Date Completed: 20260731 Latest Revision: 20260731 |
| Update Code: | 20260801 |
| DOI: | 10.1016/j.jchromb.2026.125191 |
| PMID: | 42314401 |
| Βάση Δεδομένων: | MEDLINE |
καταχωρήστε σχόλιο πρώτοι!