Academic Journal
Yam glycoprotein mediated the immunomodulation of macrophages by targeting TLR4.
| Τίτλος: | Yam glycoprotein mediated the immunomodulation of macrophages by targeting TLR4. |
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| Συγγραφείς: | Luo Y; School of Pharmacy, Xi'an Jiaotong University, No. 76 Western Yanta Road, Xi'an City, 710061, Shaanxi Province, P.R. China., Wang S; School of Pharmacy, Xi'an Jiaotong University, No. 76 Western Yanta Road, Xi'an City, 710061, Shaanxi Province, P.R. China., Liu L; School of Pharmacy, Xi'an Jiaotong University, No. 76 Western Yanta Road, Xi'an City, 710061, Shaanxi Province, P.R. China., Zhu R; School of Pharmacy, Xi'an Jiaotong University, No. 76 Western Yanta Road, Xi'an City, 710061, Shaanxi Province, P.R. China., Wang X; School of Pharmacy, Xi'an Jiaotong University, No. 76 Western Yanta Road, Xi'an City, 710061, Shaanxi Province, P.R. China., Ma Y; School of Pharmacy, Xi'an Jiaotong University, No. 76 Western Yanta Road, Xi'an City, 710061, Shaanxi Province, P.R. China., Song H; School of Pharmacy, Xi'an Jiaotong University, No. 76 Western Yanta Road, Xi'an City, 710061, Shaanxi Province, P.R. China., Wen S; School of Pharmacy, Xi'an Jiaotong University, No. 76 Western Yanta Road, Xi'an City, 710061, Shaanxi Province, P.R. China., Li L; School of Pharmacy, Xi'an Jiaotong University, No. 76 Western Yanta Road, Xi'an City, 710061, Shaanxi Province, P.R. China., Xia S; School of Pharmacy, Xi'an Jiaotong University, No. 76 Western Yanta Road, Xi'an City, 710061, Shaanxi Province, P.R. China., Li W; School of Pharmacy, Xi'an Jiaotong University, No. 76 Western Yanta Road, Xi'an City, 710061, Shaanxi Province, P.R. China. liwf@mail.xjtu.edu.cn., Niu X; School of Pharmacy, Xi'an Jiaotong University, No. 76 Western Yanta Road, Xi'an City, 710061, Shaanxi Province, P.R. China. niuxf@mail.xjtu.edu.cn. |
| Πηγή: | Molecular biology reports [Mol Biol Rep] 2026 Jun 18; Vol. 53 (1). Date of Electronic Publication: 2026 Jun 18. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Reidel Country of Publication: Netherlands NLM ID: 0403234 Publication Model: Electronic Cited Medium: Internet ISSN: 1573-4978 (Electronic) Linking ISSN: 03014851 NLM ISO Abbreviation: Mol Biol Rep Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Dordrecht, Boston, Reidel. |
| Ιατρικοί όροι (MeSH): | Macrophages*/drug effects , Macrophages*/immunology , Macrophages*/metabolism , Toll-Like Receptor 4*/metabolism , Dioscorea*/chemistry , Dioscorea*/metabolism , Immunomodulation*/drug effects , Glycoproteins*/pharmacology , Glycoproteins*/metabolism , Plant Proteins*/pharmacology, Reactive Oxygen Species/metabolism ; NF-kappa B/metabolism ; Signal Transduction/drug effects ; Cell Proliferation/drug effects ; Immunologic Factors/pharmacology ; Animals ; Mice ; RAW 264.7 Cells ; Toll-Like Receptor Agonists |
| Περίληψη: | Background: Yam is a medicinal plant and previous studies have demonstrated that the yam glycoprotein (GLP1) possessed immunomodulatory bioactivity. However, how GLP1 exerted immunomodulatory effect and the molecular mechanism is unclear. To investigate the underlying mechanisms, the immunoregulation of GLP1 on macrophages are examined. Methods and Results: The composition of GLP1 was investigated using high-performance liquid chromatography (HPLC) and UV visible spectrophotometer (UV-Vis). The activity of macrophages was detected, and the effects of GLP1 on macrophages were studied using immunofluorescence, flow cytometry, Enzyme-linked immunosorbent assay (ELISA), and Western blotting. Results indicated that GLP1, as a biomolecule, contained abundant monosaccharides and possessed O-glycopeptide bonds. GLP1 bound to pattern recognition receptors (PRRs) and was internalized by macrophages via toll-like receptor 4 (TLR4), thereby exerting its immunomodulatory effects. Further experimental studies revealed that GLP1 promoted RAW264.7 cells proliferation by advancing cells from G0/G1 phase to S phase. Additionally, GLP1 stimulated the secretion of reactive oxygen species (ROS) from mitochondria and induced macrophage polarization toward the M1 phenotype. Conclusion: GLP1 is a functional protein and has the capacity to regulate macrophages immune responses via the TLR4/NF-κB pathway. The findings highlighted the potential of GLP1 might as a dietary immunostimulant at the molecular level. (© 2026. The Author(s), under exclusive licence to Springer Nature B.V.) |
| Competing Interests: | Declarations. Competing interests: The authors declare no competing interests. |
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| Contributed Indexing: | Keywords: Immunomodulation; Macrophages; NF-κB signaling pathway; TLR4; Yam glycoprotein |
| Substance Nomenclature: | 0 (Toll-Like Receptor 4) 0 (Glycoproteins) 0 (Reactive Oxygen Species) 0 (NF-kappa B) 0 (Tlr4 protein, mouse) 0 (Immunologic Factors) 0 (Plant Proteins) 0 (Toll-Like Receptor Agonists) |
| Entry Date(s): | Date Created: 20260618 Date Completed: 20260618 Latest Revision: 20261010 |
| Update Code: | 20261010 |
| DOI: | 10.1007/s11033-026-12114-0 |
| PMID: | 42313284 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1573-4978 |
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| DOI: | 10.1007/s11033-026-12114-0 |