Defiance of stable lysogeny reveals hidden infection dynamics of phage Lambda.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος:	Defiance of stable lysogeny reveals hidden infection dynamics of phage Lambda.
Συγγραφείς:	Broux K; Department of Microbial and Molecular Systems, KU Leuven, Leuven 3001, Belgium., Bäcker LE; Department of Microbial and Molecular Systems, KU Leuven, Leuven 3001, Belgium., Oome D; Department of Microbial and Molecular Systems, KU Leuven, Leuven 3001, Belgium.; Department of Chemical Engineering, KU Leuven, Leuven 3001, Belgium., Baert F; Department of Microbial and Molecular Systems, KU Leuven, Leuven 3001, Belgium.; Department of Chemical Engineering, KU Leuven, Leuven 3001, Belgium., Vandenborre J; Department of Microbial and Molecular Systems, KU Leuven, Leuven 3001, Belgium.; Department of Chemical Engineering, KU Leuven, Leuven 3001, Belgium., Schoeters S; Department of Microbial and Molecular Systems, KU Leuven, Leuven 3001, Belgium., Tran NHL; Department of Chemical Engineering, KU Leuven, Leuven 3001, Belgium., Simoens K; Department of Chemical Engineering, KU Leuven, Leuven 3001, Belgium., Waldherr S; Department of Chemical Engineering, KU Leuven, Leuven 3001, Belgium.; Department of Functional and Evolutionary Ecology, University of Vienna, Vienna 1030, Austria., Bernaerts K; Department of Chemical Engineering, KU Leuven, Leuven 3001, Belgium., Aertsen A; Department of Microbial and Molecular Systems, KU Leuven, Leuven 3001, Belgium.
Πηγή:	Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2026 Jun 23; Vol. 123 (25), pp. e2600726123. Date of Electronic Publication: 2026 Jun 17.
Τύπος έκδοσης:	Journal Article
Γλώσσα:	English
Στοιχεία περιοδικού:	Publisher: National Academy of Sciences Country of Publication: United States NLM ID: 7505876 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1091-6490 (Electronic) Linking ISSN: 00278424 NLM ISO Abbreviation: Proc Natl Acad Sci U S A Subsets: MEDLINE
Imprint Name(s):	Original Publication: Washington, DC : National Academy of Sciences
Ιατρικοί όροι (MeSH):	Lysogeny/genetics , Bacteriophage lambda/genetics , Bacteriophage lambda*/physiology, Prophages/genetics
Περίληψη:	Stable prophage establishment is considered a quintessential response of temperate phages when faced with host cell depletion. Scrutinizing this behavior in model phage λ, however, revealed that many λ-chromosomes avoid stable integration in the host chromosome, and rather assume a nonintegrated prophage-like (or pλ) state inside the cell that keeps expressing CI-based immunity and becomes asymmetrically segregated. This creates a heterogeneous population of host cells that are either i) immune by stably carrying an integrated λ prophage, ii) immune by carrying a pλ-episome, or iii) λ-free and transiently immune by segregating from a pλ-carrier. Superinfection of these immune cells drains the number of λ-virions and creates more pλ-episomes that prolong the immunity and expansion of the λ-free subpopulation. The subsequent decline in λ-virions causes cytoplasmically inherited CI-levels in λ-free siblings to dilute out, poising them for renewed lytic consumption by λ. These overlooked pλ-dynamics attenuate the importance of prophage establishment and permit a more productive phage-host coexistence.
Competing Interests:	Competing interests statement:The authors declare no competing interest.
Grant Information:	11Q4T24N Fonds Wetenschappelijk Onderzoek (FWO); 11H0323N Fonds Wetenschappelijk Onderzoek (FWO); G0C5322N Fonds Wetenschappelijk Onderzoek (FWO); C14/20/087 Onderzoeksraad, KU Leuven (Research Council, KU Leuven)
Contributed Indexing:	Keywords: Escherichia coli; Lambda; infection dynamics; temperate phage
Entry Date(s):	Date Created: 20260617 Date Completed: 20260617 Latest Revision: 20260726
Update Code:	20260726
PubMed Central ID:	PMC13291595
DOI:	10.1073/pnas.2600726123
PMID:	42308043
Βάση Δεδομένων:	MEDLINE

Περιγραφή
ISSN:	1091-6490
DOI:	10.1073/pnas.2600726123