Academic Journal
Layer‑by‑layer biointerface engineering of biopolymer-nanoparticle hybrid coatings for durable antifouling and antibiofilm performance.
| Τίτλος: | Layer‑by‑layer biointerface engineering of biopolymer-nanoparticle hybrid coatings for durable antifouling and antibiofilm performance. |
|---|---|
| Συγγραφείς: | Ghasemi H; Department of Fisheries, Faculty of Marine Science and Technology, University of Hormozgan, Bandar Abbas, Iran., Kamrani E; Department of Fisheries, Faculty of Marine Science and Technology, University of Hormozgan, Bandar Abbas, Iran., Homaei A; Department of Marine Biology, Faculty of Marine Science and Technology, University of Hormozgan, Bandar Abbas, Iran. Electronic address: a.homaei@hormozgan.ac.ir., Zarei M; Department of Chemistry, Faculty of Sciences, University of Hormozgan, Bandar Abbas, Iran., Fernandes P; Faculty of Engineering, Lusofona University, Campo Grande, 376, Lisbon 1749-024, Portugal; iBB-Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, Lisboa 1049-001, Portugal; Associate Laboratory i4HB-Institute for Health and Bioeconomy at Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, Lisboa 1049-001, Portugal. |
| Πηγή: | Colloids and surfaces. B, Biointerfaces [Colloids Surf B Biointerfaces] 2026 Nov; Vol. 267, pp. 115912. Date of Electronic Publication: 2026 Jun 15. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 9315133 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-4367 (Electronic) Linking ISSN: 09277765 NLM ISO Abbreviation: Colloids Surf B Biointerfaces Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Amsterdam ; New York : Elsevier, c1993- |
| Ιατρικοί όροι (MeSH): | Biofilms*/drug effects , Biofouling*/prevention & control , Nanoparticles*/chemistry, Staphylococcus aureus/drug effects ; Staphylococcus aureus/physiology ; Escherichia coli/drug effects ; Escherichia coli/physiology ; Biopolymers/chemistry ; Biopolymers/pharmacology ; Layer-by-Layer Nanoparticles/chemistry ; Alginates/chemistry ; Bacterial Adhesion/drug effects ; Graphite/chemistry ; Cellulose/chemistry ; Penaeidae/enzymology ; Animals ; Surface Properties ; Particle Size |
| Περίληψη: | The layer-by-layer (LbL) assembly method using nanomaterials and naturally derived cationic and anionic polysaccharides is an actively researched field, driven to meet the demand for eco-friendly antifouling coatings and to improve current available alternatives. In this work, we present a strategy involving the activation of a polyethylene substrate to construct a four-layered coating consisting of an alginate-iron oxide nanoparticle hybrid (layer 1), bacterial cellulose (layer 2), an alginate-zinc sulfide nanoparticle hybrid (layer 3), and a protease extracted from Penaeus vannamei shrimp waste immobilized on graphene oxide nanosheets (layer 4). This assembly was deposited onto the polyethylene substrate using a polyethyleneimine adhesive sublayer via LbL self-assembly. The time required to deposit the layers in the optimal pH was measured using a design of experiments approach, which was based on atomic force microscopy and contact angle measurements. Our results indicate that layers 1 and 4 maintained their antifouling performance over time, due to the stability and low solubility of iron oxide nanoparticles and graphene oxide nanosheets. Moreover, we show that enhanced contact among each constructed layer progressively improved antifouling efficacy against bacterial adhesion (Escherichia coli and Staphylococcus aureus) and microalgal attachment (Nannochloropsis oculata). Our findings unequivocally indicate that the substantial water-binding capacity of biopolymers such as alginate and cellulose enables the fabrication of highly impermeable multilayer coatings when hybridized with nanoparticles. Overall, this study provides a straightforward and scalable approach for preparing antifouling and antimicrobial coatings from natural materials, aimed at significantly reducing biofilm growth and fouling in medical and marine applications. (Copyright © 2026 Elsevier B.V. All rights reserved.) |
| Competing Interests: | Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Biopolymer; Fouling; Layer-by-layer; Nanoparticles; Protease |
| Substance Nomenclature: | 0 (Biopolymers) 0 (Alginates) 7782-42-5 (Graphite) 9004-34-6 (Cellulose) 0 (graphene oxide) |
| Entry Date(s): | Date Created: 20260615 Date Completed: 20260811 Latest Revision: 20260811 |
| Update Code: | 20260812 |
| DOI: | 10.1016/j.colsurfb.2026.115912 |
| PMID: | 42296943 |
| Βάση Δεδομένων: | MEDLINE |
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