Academic Journal
Influence of crude extracts from different thermally treated Hysterothylacium thalassini L3 larvae on liver pathology and immune responses in C57BL/6 mice.
| Τίτλος: | Influence of crude extracts from different thermally treated Hysterothylacium thalassini L3 larvae on liver pathology and immune responses in C57BL/6 mice. |
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| Συγγραφείς: | Abdel-Gaber R; Department of Zoology, College of Science, https://ror.org/02f81g417King Saud University, P.O. 2455, Riyadh 11451, Saudi Arabia., Alotaibi M; Department of Zoology, College of Science, https://ror.org/02f81g417King Saud University, P.O. 2455, Riyadh 11451, Saudi Arabia., Albeshr M; Department of Zoology, College of Science, https://ror.org/02f81g417King Saud University, P.O. 2455, Riyadh 11451, Saudi Arabia., Dkhil MA; Department of Zoology and Entomology, Faculty of Science, https://ror.org/00h55v928Capital University (formerly Helwan University), Cairo, Egypt., Santourlidis S; Epigenetics Core Laboratory, Institute of Transplantation Diagnostics and Cell Therapeutics, Medical Faculty, https://ror.org/024z2rq82Heinrich-Heine-Universitat Düsseldorf, Düsseldorf, Germany., Elghamdy AO; Faculty of Arts and Science, https://ror.org/0403jak37Al-Baha University, Al-Baha Province, Saudi Arabia., Al-Quraishy S; Department of Zoology, College of Science, https://ror.org/02f81g417King Saud University, P.O. 2455, Riyadh 11451, Saudi Arabia., Al-Shaebi EM; Department of Zoology, College of Science, https://ror.org/02f81g417King Saud University, P.O. 2455, Riyadh 11451, Saudi Arabia. |
| Πηγή: | Journal of helminthology [J Helminthol] 2026 Jun 15; Vol. 100, pp. e64. Date of Electronic Publication: 2026 Jun 15. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: London School Of Hygiene And Tropical Medicine Country of Publication: England NLM ID: 2985115R Publication Model: Electronic Cited Medium: Internet ISSN: 1475-2697 (Electronic) Linking ISSN: 0022149X NLM ISO Abbreviation: J Helminthol Subsets: MEDLINE |
| Imprint Name(s): | Publication: London : London School Of Hygiene And Tropical Medicine Original Publication: London [etc.] |
| Ιατρικοί όροι (MeSH): | Liver*/pathology , Liver*/drug effects , Liver*/immunology , Ascaridoidea*/chemistry , Complex Mixtures*/pharmacology, Larva/chemistry ; Oxidative Stress/drug effects ; Animals ; Male ; Mice, Inbred C57BL ; Mice ; Hot Temperature |
| Περίληψη: | Hysterothylacium thalassini third-stage larvae (L3), commonly found in marine fish, are known to induce liver injury, oxidative stress, and immune disturbances when transmitted to mammalian hosts. This study investigated the pathological effects of purified crude extracts derived from fresh, heat-treated, and frozen L3 larvae on liver function, oxidative status, and immune responses in a murine model. Thirty Saurida tumbil fish were examined for L3 larvae, which were processed under three conditions: fresh (24 ± 2 °C for 60 min), heat-treated (100 °C for 60 min), and frozen (-20 °C for 24 h). Twenty male C57BL/6 mice were randomly allocated into four groups and inoculated with larval extracts at a 14-day interval. Mice exposed to fresh larval extracts exhibited significant weight loss, elevated liver enzymes (ALT, AST, ALP), hyperglycemia, and reduced total protein levels, alongside severe hepatic damage characterized by necrosis and inflammatory infiltration. Antioxidant enzyme activities (CAT and SOD) were markedly decreased, whereas oxidative stress markers (NO and H₂O₂) were increased. Additionally, there was significant upregulation of iNOS, TNF-α, and IL-10 gene expression, accompanied by elevated iNOS, IL-10, and IgE protein levels, indicating strong inflammatory and Th2-type immune responses. In contrast, extracts from heat-treated and frozen larvae resulted in attenuated pathological effects, including improved liver function, reduced oxidative stress, and moderated immune responses. These findings demonstrate that untreated L3 larval extracts exert pronounced hepatotoxic and immunomodulatory effects, while thermal and freezing treatments significantly reduce their pathogenicity and support immune homeostasis, highlighting potential strategies to mitigate parasite-induced host damage. |
| Σχόλια: | Erratum in: J Helminthol. 2026 Sep 21;100:e119. doi: 10.1017/S0022149X26102284.. (PMID: 42764779) |
| Contributed Indexing: | Keywords: Hysterothylacium thalassini; Saurida tumbil; immune modulation; liver injury; oxidative stress |
| Substance Nomenclature: | 0 (Complex Mixtures) |
| Entry Date(s): | Date Created: 20260615 Date Completed: 20260615 Latest Revision: 20260921 |
| Update Code: | 20260921 |
| DOI: | 10.1017/S0022149X26101497 |
| PMID: | 42290263 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1475-2697 |
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| DOI: | 10.1017/S0022149X26101497 |