Academic Journal
Impact of Helicobacter pylori transformation from bacillary to coccoid form on ureC gene detectability by conventional PCR: A comparative study and in silico analysis.
| Τίτλος: | Impact of Helicobacter pylori transformation from bacillary to coccoid form on ureC gene detectability by conventional PCR: A comparative study and in silico analysis. |
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| Συγγραφείς: | Oussi M; Laboratory of Helicobacter pylori and Gastric Pathologies, Institut Pasteur du Maroc, Casablanca 20360, Morocco; Biotechnology and Bioresources Valorization Laboratory, Biology Department, Faculty of Sciences, Moulay Ismail University, Meknes 11201, Morocco., Baalala A; Laboratory of Helicobacter pylori and Gastric Pathologies, Institut Pasteur du Maroc, Casablanca 20360, Morocco; Laboratory of Anthropogenetics, Biotechnology and Health, Faculty of Sciences, University Chouaib Doukkali, El Jadida 24000, Morocco., Kheir FZ; Laboratory of Helicobacter pylori and Gastric Pathologies, Institut Pasteur du Maroc, Casablanca 20360, Morocco; Biotechnology and Valorization of Plant Resources/Algae and Plants Team, Faculty of Sciences, University Chouaib Doukkali, El Jadida 24000, Morocco., Badre W; Gastroenterology and Hepatology, Ibn Rochd University Hospital Center, Casablanca 20100, Morocco., Boukhal Z; Gastroenterology and Hepatology, Ibn Rochd University Hospital Center, Casablanca 20100, Morocco., Abdelhak M; Gastro-enterologist in Private Practice, Casablanca 20340, Morocco., Harich N; Laboratory of Anthropogenetics, Biotechnology and Health, Faculty of Sciences, University Chouaib Doukkali, El Jadida 24000, Morocco., Lasky M; Biotechnology and Valorization of Plant Resources/Algae and Plants Team, Faculty of Sciences, University Chouaib Doukkali, El Jadida 24000, Morocco., Moumni M; Biotechnology and Bioresources Valorization Laboratory, Biology Department, Faculty of Sciences, Moulay Ismail University, Meknes 11201, Morocco., Boura H; Laboratory of Helicobacter pylori and Gastric Pathologies, Institut Pasteur du Maroc, Casablanca 20360, Morocco. Electronic address: hasna.boura@pasteur.ma. |
| Πηγή: | Diagnostic microbiology and infectious disease [Diagn Microbiol Infect Dis] 2026 Nov; Vol. 116 (3), pp. 117511. Date of Electronic Publication: 2026 Jun 10. |
| Τύπος έκδοσης: | Journal Article; Comparative Study |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Biomedical Country of Publication: United States NLM ID: 8305899 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0070 (Electronic) Linking ISSN: 07328893 NLM ISO Abbreviation: Diagn Microbiol Infect Dis Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: [New York, NY] : Elsevier Biomedical, [c1983- |
| Ιατρικοί όροι (MeSH): | Helicobacter pylori*/genetics , Helicobacter pylori*/isolation & purification , Polymerase Chain Reaction*/methods , Helicobacter Infections*/microbiology , Helicobacter Infections*/diagnosis , Urease*/genetics , Bacterial Proteins*/genetics , Transformation, Bacterial*, DNA, Bacterial/genetics ; Humans ; Computer Simulation |
| Περίληψη: | Helicobacter pylori is a bacterium that infects the stomach lining in half of the world's population and is responsible for 1 to 3% of stomach cancer cases. Colonization occurs through various morphologies, including the spiral bacillary and the coccoid form. The latter is often associated with environmental stress and poses significant challenges for molecular diagnosis, although it has been metabolically active. These coccoid forms contribute to increased antibiotic resistance, compromising the effectiveness of treatments and promoting persistent recurrent infections. The objective of our study is to compare the detectability of the ureC gene by conventional PCR in bacillary and coccoid forms isolated from gastric biopsy cultures on Helicobacter pylori Selective Agar. Our results confirmed the presence of the ureC gene in all bacillary forms (100%), whereas no amplification of this gene was observed in coccoid forms (0%). These results were corroborated by an in silico analysis of the complete genomes of coccoid forms, confirming the presence of the ureC gene with substitutions at the hybridization sites of the reverse primers. This study suggests that the failure to detect the ureC gene in coccoid forms may be associated with nucleotide variations in the regions targeted by standard primers, potentially affecting the efficiency of PCR amplification. These results highlight the need to evaluate more stable diagnostic targets for the detection of persistent forms of H. pylori and suggest that the transformation from the bacillary form to the coccoid form may be accompanied by genetic variability in certain regions of the ureC gene. (Copyright © 2026 Elsevier Inc. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Bacillary form; Coccoid form; Helicobacter pylori; In silico; PCR; ureC |
| Substance Nomenclature: | EC 3.5.1.5 (Urease) 0 (Bacterial Proteins) 0 (DNA, Bacterial) |
| Entry Date(s): | Date Created: 20260612 Date Completed: 20260807 Latest Revision: 20260807 |
| Update Code: | 20260808 |
| DOI: | 10.1016/j.diagmicrobio.2026.117511 |
| PMID: | 42284773 |
| Βάση Δεδομένων: | MEDLINE |
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