Academic Journal
Comparative Effects of Laparotomy and Laparoscopy-Assisted Embryo Transfer on Oxidative Stress and Thiol-Disulphide Homeostasis in Tuj Ewes.
| Τίτλος: | Comparative Effects of Laparotomy and Laparoscopy-Assisted Embryo Transfer on Oxidative Stress and Thiol-Disulphide Homeostasis in Tuj Ewes. |
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| Συγγραφείς: | Demir MC; Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Kafkas University, Kars, Türkiye., Demir MS; Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Kafkas University, Kars, Türkiye., Makav M; Department of Physiology, Faculty of Veterinary Medicine, Kafkas University, Kars, Türkiye., Kaya S; Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Kafkas University, Kars, Türkiye., Gökdemir T; Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Kafkas University, Kars, Türkiye., Önder NT; Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Kafkas University, Kars, Türkiye., Kuru M; Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Kafkas University, Kars, Türkiye., Kaçar C; Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Kafkas University, Kars, Türkiye. |
| Πηγή: | Veterinary medicine and science [Vet Med Sci] 2026 Jul; Vol. 12 (4), pp. e71023. |
| Τύπος έκδοσης: | Journal Article; Comparative Study |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: John Wiley & Sons Ltd Country of Publication: England NLM ID: 101678837 Publication Model: Print Cited Medium: Internet ISSN: 2053-1095 (Electronic) Linking ISSN: 20531095 NLM ISO Abbreviation: Vet Med Sci Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: [Oxford] : John Wiley & Sons Ltd., [2015]- |
| Ιατρικοί όροι (MeSH): | Laparoscopy*/veterinary , Disulfides*/blood , Disulfides*/metabolism , Sulfhydryl Compounds*/blood , Sulfhydryl Compounds*/metabolism , Embryo Transfer*/veterinary , Embryo Transfer*/methods , Laparotomy*/veterinary , Oxidative Stress*, Sheep/physiology ; Glutathione/blood ; Animals ; Female ; Homeostasis |
| Περίληψη: | Objectives: In this study, it was aimed to compare the effects of embryo transfer performed via laparotomy and laparoscopy on thiol-disulphide homeostasis and some oxidative stress markers in Tuj ewes. Methods: In the study, 10 Tuj ewes were randomly divided into two groups (laparotomy, n = 5; laparoscopy, n = 5). A progesterone-based synchronization protocol was applied to all animals. Blood samples were collected immediately before surgery, immediately after surgery, and at 24 and 48 h postoperatively. Serum levels of glutathione (GSH), malondialdehyde (MDA), total thiol (TT), native thiol (NT), disulphide (Ds) and thiol-disulphide ratios were determined. Results: Group, time, and group × time interactions were found to be statistically significant for GSH, MDA, TT, and NT parameters (p < 0.05). In the laparotomy group, GSH, TT, and NT levels markedly decreased during the postoperative period (p < 0.05), whereas MDA levels showed a significant increase, particularly at 48 h (p < 0.05). In the laparoscopy group, changes in oxidative stress markers were found to be more limited. Although no significant differences were observed between the groups in terms of disulphide levels and thiol-disulphide ratios (p > 0.05), time-dependent changes in these parameters were statistically significant (p < 0.05). In the laparotomy group, significant negative correlations were detected between MDA and antioxidant parameters (p < 0.05), while strong positive correlations were observed among thiol parameters (p < 0.01). Conclusions: As a result, it was concluded that the surgical approach used in embryo transfer affects the systemic oxidative stress response and that thiol-disulphide homeostasis can be considered a sensitive biomarker for revealing these differences. It was determined that the laparoscopic embryo transfer method disrupts redox balance to a lesser extent and better preserves the antioxidant defence system compared with laparotomy. (© 2026 The Author(s). Veterinary Medicine and Science published by John Wiley & Sons Ltd.) |
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| Contributed Indexing: | Keywords: embryo transfer; laparoscopy; laparotomy; oxidative stress; sheep; thiol–disulphide homeostasis |
| Substance Nomenclature: | 0 (Disulfides) 0 (Sulfhydryl Compounds) GAN16C9B8O (Glutathione) |
| Entry Date(s): | Date Created: 20260612 Date Completed: 20260612 Latest Revision: 20260813 |
| Update Code: | 20260813 |
| PubMed Central ID: | PMC13261301 |
| DOI: | 10.1002/vms3.71023 |
| PMID: | 42281509 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 2053-1095 |
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| DOI: | 10.1002/vms3.71023 |