Academic Journal
Production and Structural/Physicochemical Characteristics of Exopolysaccharides from the Halotolerant Strain Oceanobacillus sp. DT-Z6.
| Τίτλος: | Production and Structural/Physicochemical Characteristics of Exopolysaccharides from the Halotolerant Strain Oceanobacillus sp. DT-Z6. |
|---|---|
| Συγγραφείς: | Huang Y; Institute of Fungus Resources, Guizhou Key Laboratory of Agricultural Microbiology/Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences, Guizhou University, Guiyang, Guizhou Province, 550025, China., Wang C; Institute of Fungus Resources, Guizhou Key Laboratory of Agricultural Microbiology/Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences, Guizhou University, Guiyang, Guizhou Province, 550025, China., Gan L; Institute of Fungus Resources, Guizhou Key Laboratory of Agricultural Microbiology/Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences, Guizhou University, Guiyang, Guizhou Province, 550025, China. lzgan@gzu.edu.cn., Dong C; Institute of Fungus Resources, Guizhou Key Laboratory of Agricultural Microbiology/Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences, Guizhou University, Guiyang, Guizhou Province, 550025, China., Tian Y; Key Laboratory of Leather Chemistry and Engineering (Ministry of Education), College of Biomass Science and Engineering, Sichuan University, Chengdu, Sichuan Province, 610065, China., Zou X; Institute of Fungus Resources, Guizhou Key Laboratory of Agricultural Microbiology/Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences, Guizhou University, Guiyang, Guizhou Province, 550025, China. xzou@gzu.edu.cn. |
| Πηγή: | Applied biochemistry and biotechnology [Appl Biochem Biotechnol] 2026 Sep; Vol. 198 (9), pp. 7117-7137. Date of Electronic Publication: 2026 Jun 11. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Humana Press Country of Publication: United States NLM ID: 8208561 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1559-0291 (Electronic) Linking ISSN: 02732289 NLM ISO Abbreviation: Appl Biochem Biotechnol Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Clifton, N.J. : Humana Press, c1981- |
| Ιατρικοί όροι (MeSH): | Polysaccharides, Bacterial*/chemistry , Polysaccharides, Bacterial*/biosynthesis , Bacillaceae*/metabolism, Fructans/chemistry ; Spectroscopy, Fourier Transform Infrared ; Molecular Weight ; Fermentation |
| Περίληψη: | Halotolerant strains isolated from extreme environments rely on exopolysaccharides (EPSs) to maintain cellular integrity under high salinity and ensure survival under harsh conditions. Although continuous screening efforts have identified numerous EPS-producing halotolerant strains, research on the structural characteristics and physicochemical properties of their EPSs remains limited. In this study, an EPS-producing strain yielding 2.59 ± 0.15 g/L was isolated from saline soil and identified as Oceanobacillus sp. DT-Z6. The optimal conditions for key fermentation parameters were determined using a one-factor-at-a-time approach, resulting in a maximum EPS yield of 6.65 ± 0.06 g/L. EPSs produced under optimized conditions were extracted by ethanol precipitation and further purified using anion-exchange chromatography and gel filtration chromatography, yielding two novel EPS fractions, EPS6-0 and EPS6-1. The purified EPSs were characterized using monosaccharide composition analysis, Fourier-transform infrared spectroscopy, and nuclear magnetic resonance spectroscopy. The results indicated that both EPS6-0 and EPS6-1 were levan-type fructans containing β-(2, 6) linkages, with molecular weights of 1.17 × 108 g/mol and 5.93 × 107 g/mol, respectively. Physicochemical analysis revealed that EPS6-0 and EPS6-1 exhibit narrow size distributions in solutions, diverse micro-morphologies, and excellent thermal stability. Collectively, these findings provide a foundation for understanding structure-function relationships in EPSs from this halotolerant strain and support their potential industrial applications. (© 2026. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.) |
| Competing Interests: | Declarations. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
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| Grant Information: | No. 32460033 National Natural Science Foundation of China; No. Qianjiaoji (2024) 35 Guizhou Provincial Department of Education Youth Science and Technology Talent Development Project; No. Qiankehe Foundation-ZK (2024) General 089 Guizhou Provincial Basic Research Program (Natural Science) |
| Contributed Indexing: | Keywords: Oceanobacillus sp; Biopolymer production; Exopolysaccharides; Extremophiles; Levan |
| Substance Nomenclature: | 0 (Polysaccharides, Bacterial) 0 (Fructans) 9013-95-0 (levan) |
| Entry Date(s): | Date Created: 20260611 Date Completed: 20260912 Latest Revision: 20260912 |
| Update Code: | 20260913 |
| DOI: | 10.1007/s12010-026-05781-z |
| PMID: | 42274876 |
| Βάση Δεδομένων: | MEDLINE |
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