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Production and Structural/Physicochemical Characteristics of Exopolysaccharides from the Halotolerant Strain Oceanobacillus sp. DT-Z6.

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Title: Production and Structural/Physicochemical Characteristics of Exopolysaccharides from the Halotolerant Strain Oceanobacillus sp. DT-Z6.
Authors: Huang Y; Institute of Fungus Resources, Guizhou Key Laboratory of Agricultural Microbiology/Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences, Guizhou University, Guiyang, Guizhou Province, 550025, China., Wang C; Institute of Fungus Resources, Guizhou Key Laboratory of Agricultural Microbiology/Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences, Guizhou University, Guiyang, Guizhou Province, 550025, China., Gan L; Institute of Fungus Resources, Guizhou Key Laboratory of Agricultural Microbiology/Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences, Guizhou University, Guiyang, Guizhou Province, 550025, China. lzgan@gzu.edu.cn., Dong C; Institute of Fungus Resources, Guizhou Key Laboratory of Agricultural Microbiology/Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences, Guizhou University, Guiyang, Guizhou Province, 550025, China., Tian Y; Key Laboratory of Leather Chemistry and Engineering (Ministry of Education), College of Biomass Science and Engineering, Sichuan University, Chengdu, Sichuan Province, 610065, China., Zou X; Institute of Fungus Resources, Guizhou Key Laboratory of Agricultural Microbiology/Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences, Guizhou University, Guiyang, Guizhou Province, 550025, China. xzou@gzu.edu.cn.
Source: Applied biochemistry and biotechnology [Appl Biochem Biotechnol] 2026 Sep; Vol. 198 (9), pp. 7117-7137. Date of Electronic Publication: 2026 Jun 11.
Publication Type: Journal Article
Language: English
Journal Info: Publisher: Humana Press Country of Publication: United States NLM ID: 8208561 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1559-0291 (Electronic) Linking ISSN: 02732289 NLM ISO Abbreviation: Appl Biochem Biotechnol Subsets: MEDLINE
Imprint Name(s): Original Publication: Clifton, N.J. : Humana Press, c1981-
MeSH Terms: Polysaccharides, Bacterial*/chemistry , Polysaccharides, Bacterial*/biosynthesis , Bacillaceae*/metabolism, Fructans/chemistry ; Spectroscopy, Fourier Transform Infrared ; Molecular Weight ; Fermentation
Abstract: Halotolerant strains isolated from extreme environments rely on exopolysaccharides (EPSs) to maintain cellular integrity under high salinity and ensure survival under harsh conditions. Although continuous screening efforts have identified numerous EPS-producing halotolerant strains, research on the structural characteristics and physicochemical properties of their EPSs remains limited. In this study, an EPS-producing strain yielding 2.59 ± 0.15 g/L was isolated from saline soil and identified as Oceanobacillus sp. DT-Z6. The optimal conditions for key fermentation parameters were determined using a one-factor-at-a-time approach, resulting in a maximum EPS yield of 6.65 ± 0.06 g/L. EPSs produced under optimized conditions were extracted by ethanol precipitation and further purified using anion-exchange chromatography and gel filtration chromatography, yielding two novel EPS fractions, EPS6-0 and EPS6-1. The purified EPSs were characterized using monosaccharide composition analysis, Fourier-transform infrared spectroscopy, and nuclear magnetic resonance spectroscopy. The results indicated that both EPS6-0 and EPS6-1 were levan-type fructans containing β-(2, 6) linkages, with molecular weights of 1.17 × 108 g/mol and 5.93 × 107 g/mol, respectively. Physicochemical analysis revealed that EPS6-0 and EPS6-1 exhibit narrow size distributions in solutions, diverse micro-morphologies, and excellent thermal stability. Collectively, these findings provide a foundation for understanding structure-function relationships in EPSs from this halotolerant strain and support their potential industrial applications.
(© 2026. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Competing Interests: Declarations. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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Grant Information: No. 32460033 National Natural Science Foundation of China; No. Qianjiaoji (2024) 35 Guizhou Provincial Department of Education Youth Science and Technology Talent Development Project; No. Qiankehe Foundation-ZK (2024) General 089 Guizhou Provincial Basic Research Program (Natural Science)
Contributed Indexing: Keywords: Oceanobacillus sp; Biopolymer production; Exopolysaccharides; Extremophiles; Levan
Substance Nomenclature: 0 (Polysaccharides, Bacterial)
0 (Fructans)
9013-95-0 (levan)
Entry Date(s): Date Created: 20260611 Date Completed: 20260912 Latest Revision: 20260912
Update Code: 20260913
DOI: 10.1007/s12010-026-05781-z
PMID: 42274876
Database: MEDLINE
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  – Url: https://resolver.ebsco.com/c/fiv2js/result?sid=EBSCO:cmedm&genre=article&issn=15590291&ISBN=&volume=198&issue=9&date=20260901&spage=7117&pages=7117-7137&title=Applied biochemistry and biotechnology&atitle=Production%20and%20Structural%2FPhysicochemical%20Characteristics%20of%20Exopolysaccharides%20from%20the%20Halotolerant%20Strain%20Oceanobacillus%20sp.%20DT-Z6.&aulast=Huang%20Y&id=DOI:10.1007/s12010-026-05781-z
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Items – Name: Title
  Label: Title
  Group: Ti
  Data: Production and Structural/Physicochemical Characteristics of Exopolysaccharides from the Halotolerant Strain Oceanobacillus sp. DT-Z6.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AU" term="%22Huang+Y%22">Huang Y</searchLink>; Institute of Fungus Resources, Guizhou Key Laboratory of Agricultural Microbiology/Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences, Guizhou University, Guiyang, Guizhou Province, 550025, China.<br /><searchLink fieldCode="AU" term="%22Wang+C%22">Wang C</searchLink>; Institute of Fungus Resources, Guizhou Key Laboratory of Agricultural Microbiology/Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences, Guizhou University, Guiyang, Guizhou Province, 550025, China.<br /><searchLink fieldCode="AU" term="%22Gan+L%22">Gan L</searchLink>; Institute of Fungus Resources, Guizhou Key Laboratory of Agricultural Microbiology/Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences, Guizhou University, Guiyang, Guizhou Province, 550025, China. lzgan@gzu.edu.cn.<br /><searchLink fieldCode="AU" term="%22Dong+C%22">Dong C</searchLink>; Institute of Fungus Resources, Guizhou Key Laboratory of Agricultural Microbiology/Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences, Guizhou University, Guiyang, Guizhou Province, 550025, China.<br /><searchLink fieldCode="AU" term="%22Tian+Y%22">Tian Y</searchLink>; Key Laboratory of Leather Chemistry and Engineering (Ministry of Education), College of Biomass Science and Engineering, Sichuan University, Chengdu, Sichuan Province, 610065, China.<br /><searchLink fieldCode="AU" term="%22Zou+X%22">Zou X</searchLink>; Institute of Fungus Resources, Guizhou Key Laboratory of Agricultural Microbiology/Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences, Guizhou University, Guiyang, Guizhou Province, 550025, China. xzou@gzu.edu.cn.
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%228208561%22">Applied biochemistry and biotechnology</searchLink> [Appl Biochem Biotechnol] 2026 Sep; Vol. 198 (9), pp. 7117-7137. <i>Date of Electronic Publication: </i>2026 Jun 11.
– Name: TypePub
  Label: Publication Type
  Group: TypPub
  Data: Journal Article
– Name: Language
  Label: Language
  Group: Lang
  Data: English
– Name: TitleSource
  Label: Journal Info
  Group: Src
  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22Humana+Press%22">Humana Press </searchLink><i>Country of Publication: </i>United States <i>NLM ID: </i>8208561 <i>Publication Model: </i>Print-Electronic <i>Cited Medium: </i>Internet <i>ISSN: </i>1559-0291 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2202732289%22">02732289 </searchLink><i>NLM ISO Abbreviation: </i>Appl Biochem Biotechnol <i>Subsets: </i>MEDLINE
– Name: PublisherInfo
  Label: Imprint Name(s)
  Group: PubInfo
  Data: <i>Original Publication</i>: Clifton, N.J. : Humana Press, c1981-
– Name: SubjectMESH
  Label: MeSH Terms
  Group: Su
  Data: <searchLink fieldCode="MM" term="%22Polysaccharides%2C+Bacterial%22">Polysaccharides, Bacterial*</searchLink>/<searchLink fieldCode="MM" term="%22Polysaccharides%2C+Bacterial+chemistry%22">chemistry</searchLink> <br /><searchLink fieldCode="MM" term="%22Polysaccharides%2C+Bacterial%22">Polysaccharides, Bacterial*</searchLink>/<searchLink fieldCode="MM" term="%22Polysaccharides%2C+Bacterial+biosynthesis%22">biosynthesis</searchLink> <br /><searchLink fieldCode="MM" term="%22Bacillaceae%22">Bacillaceae*</searchLink>/<searchLink fieldCode="MM" term="%22Bacillaceae+metabolism%22">metabolism</searchLink><br /><searchLink fieldCode="MH" term="%22Fructans%22">Fructans</searchLink>/<searchLink fieldCode="MH" term="%22Fructans+chemistry%22">chemistry</searchLink> ; <searchLink fieldCode="MH" term="%22Spectroscopy%2C+Fourier+Transform+Infrared%22">Spectroscopy, Fourier Transform Infrared</searchLink> ; <searchLink fieldCode="MH" term="%22Molecular+Weight%22">Molecular Weight</searchLink> ; <searchLink fieldCode="MH" term="%22Fermentation%22">Fermentation</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Halotolerant strains isolated from extreme environments rely on exopolysaccharides (EPSs) to maintain cellular integrity under high salinity and ensure survival under harsh conditions. Although continuous screening efforts have identified numerous EPS-producing halotolerant strains, research on the structural characteristics and physicochemical properties of their EPSs remains limited. In this study, an EPS-producing strain yielding 2.59 ± 0.15 g/L was isolated from saline soil and identified as Oceanobacillus sp. DT-Z6. The optimal conditions for key fermentation parameters were determined using a one-factor-at-a-time approach, resulting in a maximum EPS yield of 6.65 ± 0.06 g/L. EPSs produced under optimized conditions were extracted by ethanol precipitation and further purified using anion-exchange chromatography and gel filtration chromatography, yielding two novel EPS fractions, EPS6-0 and EPS6-1. The purified EPSs were characterized using monosaccharide composition analysis, Fourier-transform infrared spectroscopy, and nuclear magnetic resonance spectroscopy. The results indicated that both EPS6-0 and EPS6-1 were levan-type fructans containing β-(2, 6) linkages, with molecular weights of 1.17 × 10<superscript>8</superscript> g/mol and 5.93 × 10<superscript>7</superscript> g/mol, respectively. Physicochemical analysis revealed that EPS6-0 and EPS6-1 exhibit narrow size distributions in solutions, diverse micro-morphologies, and excellent thermal stability. Collectively, these findings provide a foundation for understanding structure-function relationships in EPSs from this halotolerant strain and support their potential industrial applications.<br /> (© 2026. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
– Name: Abstract
  Label: Competing Interests
  Group: Ab
  Data: Declarations. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
– Name: Ref
  Label: References
  Group: RefInfo
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  Data: No. 32460033 National Natural Science Foundation of China; No. Qianjiaoji (2024) 35 Guizhou Provincial Department of Education Youth Science and Technology Talent Development Project; No. Qiankehe Foundation-ZK (2024) General 089 Guizhou Provincial Basic Research Program (Natural Science)
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  Data: <i>Keywords: </i>Oceanobacillus sp; Biopolymer production; Exopolysaccharides; Extremophiles; Levan
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              Text: 2026 Sep
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