Academic Journal
Ultra-High-Throughput Discovery of Multifunctional Polyphenolic Coatings on Droplet Microarrays.
| Τίτλος: | Ultra-High-Throughput Discovery of Multifunctional Polyphenolic Coatings on Droplet Microarrays. |
|---|---|
| Συγγραφείς: | Widyaya VT; Institute of Biological and Chemical Systems-Functional Molecular Systems (IBCS-FMS), Karlsruhe Institute of Technology (KIT), Eggenstein-Leopoldshafen, Germany., Gómez JEU; Institute of Automation and Applied Informatics (IAI), Karlsruhe Institute of Technology (KIT), Eggenstein-Leopoldshafen, Germany., Reuß P; Institute of Biological and Chemical Systems-Functional Molecular Systems (IBCS-FMS), Karlsruhe Institute of Technology (KIT), Eggenstein-Leopoldshafen, Germany., Welle A; Institute of Functional Interfaces (IFG), Karlsruhe Institute of Technology (KIT), Eggenstein-Leopoldshafen, Germany.; Karlsruhe Nano Micro Facility (KNMF), Karlsruhe Institute of Technology (KIT), Eggenstein-Leopoldshafen, Germany., Gattung RA; Institute of Automation and Applied Informatics (IAI), Karlsruhe Institute of Technology (KIT), Eggenstein-Leopoldshafen, Germany., Mayer J; Institute of Biological and Chemical Systems-Functional Molecular Systems (IBCS-FMS), Karlsruhe Institute of Technology (KIT), Eggenstein-Leopoldshafen, Germany., Krolla P; Institute of Functional Interfaces (IFG), Karlsruhe Institute of Technology (KIT), Eggenstein-Leopoldshafen, Germany., Reischl M; Institute of Automation and Applied Informatics (IAI), Karlsruhe Institute of Technology (KIT), Eggenstein-Leopoldshafen, Germany., Friederich P; Institute of Nanotechnology (INT), Karlsruhe Institute of Technology (KIT), Eggenstein-Leopoldshafen, Germany.; Institute of Theoretical Informatics (ITI), Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany., Popova AA; Institute of Biological and Chemical Systems-Functional Molecular Systems (IBCS-FMS), Karlsruhe Institute of Technology (KIT), Eggenstein-Leopoldshafen, Germany., Schwartz T; Institute of Functional Interfaces (IFG), Karlsruhe Institute of Technology (KIT), Eggenstein-Leopoldshafen, Germany., Levkin PA; Institute of Biological and Chemical Systems-Functional Molecular Systems (IBCS-FMS), Karlsruhe Institute of Technology (KIT), Eggenstein-Leopoldshafen, Germany.; Institute of Organic Chemistry (IOC), Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany. |
| Πηγή: | Advanced materials (Deerfield Beach, Fla.) [Adv Mater] 2026 Jul; Vol. 38 (39), pp. e73612. Date of Electronic Publication: 2026 Jun 11. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 9885358 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1521-4095 (Electronic) Linking ISSN: 09359648 NLM ISO Abbreviation: Adv Mater Subsets: MEDLINE |
| Imprint Name(s): | Publication: Sept. 3, 1997- : Weinheim : Wiley-VCH Original Publication: Deerfield Beach, FL : VCH Publishers, 1989- |
| Ιατρικοί όροι (MeSH): | Polyphenols*/chemistry , Polyphenols*/pharmacology , Anti-Bacterial Agents*/chemistry , Anti-Bacterial Agents*/pharmacology , Coated Materials, Biocompatible*/chemistry , Coated Materials, Biocompatible*/pharmacology , High-Throughput Screening Assays*/methods , Microarray Analysis*/methods, Pseudomonas aeruginosa/drug effects ; Escherichia coli/drug effects ; Polyamines/chemistry ; Humans ; Surface Properties |
| Περίληψη: | The rapid discovery of functional coatings is vital for advancing technologies in healthcare, energy, and environmental protection, yet it remains limited by the lack of scalable high-throughput (HT) methods. Here, an ultra-high-throughput (UHT) combinatorial strategy is introduced for the miniaturized synthesis and screening of polyamine-polyphenolic (PaPp) coatings (formed in Tris buffer) using droplet microarrays (DMA). Approximately 30 000 coatings were generated from binary and ternary combinations of 51 polyphenols (Pp) and 12 polyamines (Pa), each produced in 160 nL volumes (<5 mL total reagent use), enabling the rapid identification of hundreds of previously unknown functional materials, including over 225 fluorescent coatings and more than 100 redox-active, metal-reducing surfaces. Antibacterial screening uncovered seven coatings with reproducible activity against Pseudomonas aeruginosa, and subsequent validation on macroscale substrates confirmed up to <1-log colony-forming unit (CFU) reduction against P. aeruginosa and E. coli. Importantly, five coatings exhibited multiple functionalities, combining surface stability, intrinsic fluorescence, metal-reducing activity, antibacterial effects, and compatibility with adherent human cells. This UHT approach yields the first comprehensive functionality map of PaPp coatings, revealing previously inaccessible multifunctional materials and demonstrating a scalable strategy for the discovery of surface chemistries with tailored optical, redox, and biological properties. (© 2026 The Author(s). Advanced Materials published by Wiley‐VCH GmbH.) |
| References: | Nat Commun. 2020 Oct 26;11(1):5391. (PMID: 33106489) Am J Infect Control. 2013 May;41(5 Suppl):S72-6. (PMID: 23622754) Nat Prod Res. 2022 Dec;36(24):6453-6458. (PMID: 35142575) Crit Rev Food Sci Nutr. 2024 Nov;64(31):11341-11355. (PMID: 37486163) ACS Omega. 2024 Feb 08;9(7):7725-7736. (PMID: 38405455) Adv Mater. 2024 Feb;36(6):e2307680. (PMID: 37997498) J Cell Physiol. 2000 Mar;182(3):311-22. (PMID: 10653597) Biotechnol Biofuels. 2019 Nov 18;12:271. (PMID: 31832094) Small. 2024 Jun;20(26):e2307215. (PMID: 38258390) Compr Rev Food Sci Food Saf. 2024 Jan;23(1):e13275. (PMID: 38284604) Materials (Basel). 2021 May 01;14(9):. (PMID: 34062785) Chem Soc Rev. 2018 Nov 26;47(23):8842-8880. (PMID: 30361725) Sci Rep. 2021 Nov 8;11(1):21836. (PMID: 34750447) Antimicrob Agents Chemother. 2017 Sep 22;61(10):. (PMID: 28784676) Small. 2022 Dec;18(48):e2204388. (PMID: 36253133) Adv Mater. 2020 Oct;32(43):e2002118. (PMID: 32844497) Chempluschem. 2024 May;89(5):e202300695. (PMID: 38251920) Microbiol Res. 2003;158(4):281-9. (PMID: 14717448) Int J Biol Macromol. 2021 May 1;178:19-28. (PMID: 33636258) ACS Omega. 2022 Jan 04;7(2):1595-1601. (PMID: 35071855) Langmuir. 2019 Feb 5;35(5):1211-1226. (PMID: 30563333) Macromol Rapid Commun. 2023 Aug;44(16):e2200946. (PMID: 36758219) Adv Healthc Mater. 2025 Jan;14(1):e2401820. (PMID: 39444094) Molecules. 2020 Oct 02;25(19):. (PMID: 33023106) Small. 2020 Mar;16(10):e1905971. (PMID: 31985878) ACS Appl Mater Interfaces. 2024 Feb 21;16(7):9182-9189. (PMID: 38343193) J Mater Chem B. 2015 Aug 14;3(30):6224-6238. (PMID: 32262741) Lancet. 2024 Sep 28;404(10459):1199-1226. (PMID: 39299261) Adv Food Nutr Res. 2021;98:281-320. (PMID: 34507645) Macromol Biosci. 2012 Sep;12(9):1279-89. (PMID: 22865776) Crit Rev Food Sci Nutr. 2012;52(3):213-48. (PMID: 22214442) Sci Rep. 2022 Mar 2;12(1):3460. (PMID: 35236912) Pharmaceuticals (Basel). 2025 Mar 15;18(3):. (PMID: 40143193) FEMS Microbiol Ecol. 2006 Jul;57(1):158-67. (PMID: 16819959) Antioxidants (Basel). 2019 Dec 21;9(1):. (PMID: 31877807) J Electron Spectros Relat Phenomena. 2009 May 1;172(1-3):2-8. (PMID: 20161353) PeerJ. 2014 Jun 19;2:e453. (PMID: 25024921) Biomed Res Int. 2018 May 9;2018:8308640. (PMID: 29854796) Small. 2024 Jan;20(4):e2304325. (PMID: 37726239) Adv Mater. 2024 Mar;36(13):e2306960. (PMID: 37718555) Carbohydr Polym. 2022 Nov 15;296:119892. (PMID: 36087967) ACS Appl Bio Mater. 2022 Apr 18;5(4):1391-1463. (PMID: 35358388) Adv Biol (Weinh). 2022 Dec;6(12):e2200166. (PMID: 35843867) Int J Biol Macromol. 2024 Jun;271(Pt 2):132511. (PMID: 38772471) BMC Bioinformatics. 2021 Sep 10;22(1):433. (PMID: 34507520) Int J Pharm. 2021 May 15;601:120525. (PMID: 33781878) ACS Biomater Sci Eng. 2019 Sep 9;5(9):4272-4284. (PMID: 33417783) Plants (Basel). 2023 Mar 07;12(6):. (PMID: 36986905) Pharmaceuticals (Basel). 2023 Jan 10;16(1):. (PMID: 36678599) Oxid Med Cell Longev. 2009 Nov-Dec;2(5):270-8. (PMID: 20716914) Antibiotics (Basel). 2022 Jan 30;11(2):. (PMID: 35203785) Angew Chem Int Ed Engl. 2012 Jul 27;51(31):7752-5. (PMID: 22744868) Adv Healthc Mater. 2022 Jun;11(12):e2102493. (PMID: 35285171) Mater Today Bio. 2021 Mar 10;10:100108. (PMID: 33912825) Int J Biol Macromol. 2024 Mar;262(Pt 1):130003. (PMID: 38325696) Adv Mater. 2024 Jan;36(3):e2307727. (PMID: 37820045) RSC Adv. 2022 Mar 10;12(13):7689-7711. (PMID: 35424749) Nature. 2024 Aug;632(8025):494-496. (PMID: 39138306) Small Methods. 2023 Sep;7(9):e2300553. (PMID: 37287430) Angew Chem Int Ed Engl. 2013 Oct 4;52(41):10766-70. (PMID: 24027124) Front Microbiol. 2019 Apr 18;10:829. (PMID: 31057527) Biomed Pharmacother. 2023 Sep;165:115170. (PMID: 37481930) Small. 2023 Mar;19(9):e2204512. (PMID: 36538723) Anal Methods. 2024 Apr 18;16(15):2198-2228. (PMID: 38567418) Biomolecules. 2022 Jul 09;12(7):. (PMID: 35883519) Curr Med Chem. 2020;27(15):2576-2606. (PMID: 30295182) J Mater Chem B. 2020 Apr 21;8(15):2951-2973. (PMID: 32159205) J Microbiol Methods. 2023 Jan;204:106656. (PMID: 36526040) Int J Nanomedicine. 2024 Nov 06;19:11379-11395. (PMID: 39524920) J Nanobiotechnology. 2024 Nov 21;22(1):725. (PMID: 39574119) Angew Chem Int Ed Engl. 2014 May 26;53(22):5546-51. (PMID: 24700671) J Nanobiotechnology. 2024 Aug 8;22(1):471. (PMID: 39118143) J Mater Chem B. 2024 Sep 18;12(36):8883-8896. (PMID: 39177039) ACS Omega. 2024 Mar 15;9(12):14475-14488. (PMID: 38559964) Macromol Rapid Commun. 2024 Dec;45(23):e2400345. (PMID: 38760014) ACS Nano. 2023 Nov 28;17(22):22355-22370. (PMID: 37930078) ACS Comb Sci. 2016 Sep 12;18(9):507-26. (PMID: 27440008) Small. 2020 Dec;16(52):e2004575. (PMID: 33216442) J Hazard Mater. 2024 Dec 5;480:135873. (PMID: 39305594) |
| Grant Information: | 501386634 Deutsche Forschungsgemeinschaft; 406232485 DFG Heisenbergprofessur grant; LE 2936/9-1 DFG Heisenbergprofessur grant |
| Contributed Indexing: | Keywords: antimicrobial resistance; droplet microarray technology; functional coatings; plant polyphenols; polyamine‐polyphenolic coatings; surface chemistry; surface functionalization; ultra‐high‐throughput combinatorial synthesis; ultra‐high‐throughput screening |
| Substance Nomenclature: | 0 (Polyphenols) 0 (Anti-Bacterial Agents) 0 (Polyamines) 0 (Coated Materials, Biocompatible) |
| Entry Date(s): | Date Created: 20260611 Date Completed: 20260713 Latest Revision: 20260813 |
| Update Code: | 20260813 |
| PubMed Central ID: | PMC13361275 |
| DOI: | 10.1002/adma.73612 |
| PMID: | 42272440 |
| Βάση Δεδομένων: | MEDLINE |
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