Academic Journal
Multi-wavelength laser-induced fluorescence spectroscopy with KLDA and Fisher score feature selection for pollen classification.
| Τίτλος: | Multi-wavelength laser-induced fluorescence spectroscopy with KLDA and Fisher score feature selection for pollen classification. |
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| Συγγραφείς: | Guo P, You Y, Chen S, Chen H, Jiang Y, Shi X, Gong J, Gong W |
| Πηγή: | Applied optics [Appl Opt] 2026 Jun 01; Vol. 65 (16), pp. 5259-5269. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Optica Publishing Group Country of Publication: United States NLM ID: 0247660 Publication Model: Print Cited Medium: Internet ISSN: 1539-4522 (Electronic) Linking ISSN: 1559128X NLM ISO Abbreviation: Appl Opt Subsets: MEDLINE |
| Imprint Name(s): | Publication: Washington, DC : Optica Publishing Group Original Publication: Easton, Pa., Optical Society of America. |
| Ιατρικοί όροι (MeSH): | Pollen*/classification , Pollen*/chemistry , Classification Algorithms* , Lasers*, Spectrometry, Fluorescence/instrumentation ; Spectrometry, Fluorescence/methods ; Discriminant Analysis ; Random Forest |
| Περίληψη: | This study proposes a method for pollen classification using three-wavelength laser-induced fluorescence (LIF) spectroscopy combined with kernelized linear discriminant analysis (KLDA). Fluorescence spectra were acquired from eight pollen species using LIF with excitation wavelengths of 266, 360, and 405 nm. Fisher score was applied to remove redundant features and identify representative spectral information. The selected features were then subjected to KLDA for dimensionality reduction, and classification was performed using a Random Forest algorithm. The proposed method achieved a classification accuracy of 100% across all pollen species tested. Among the three excitation wavelengths, the 405 nm wavelength provided the most distinct spectral features. Integrating spectral data from all three wavelengths further enhanced classification performance. The results demonstrate that combining multi-wavelength LIF spectroscopy with KLDA effectively differentiates pollen types based on fluorescence spectral characteristics. |
| Entry Date(s): | Date Created: 20260610 Date Completed: 20260612 Latest Revision: 20260710 |
| Update Code: | 20260711 |
| DOI: | 10.1364/AO.585306 |
| PMID: | 42267993 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1539-4522 |
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| DOI: | 10.1364/AO.585306 |