Academic Journal
Thiolated Chitosan-Stearic Acid Thiol-Linked Biomimetic Hydrogels for Axonal Regrowth and Neuronal Tissue Regeneration.
| Title: | Thiolated Chitosan-Stearic Acid Thiol-Linked Biomimetic Hydrogels for Axonal Regrowth and Neuronal Tissue Regeneration. |
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| Authors: | Bhatt M; Department of Biomedical Engineering, Indian Institute of Technology Ropar, Rupnagar, Punjab 140001, India., Das B; Department of Biomedical Engineering, Indian Institute of Technology Ropar, Rupnagar, Punjab 140001, India. |
| Source: | ACS applied bio materials [ACS Appl Bio Mater] 2026 Jul 06; Vol. 9 (13), pp. 5557-5573. Date of Electronic Publication: 2026 Jun 08. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: ACS Publications Country of Publication: United States NLM ID: 101729147 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2576-6422 (Electronic) Linking ISSN: 25766422 NLM ISO Abbreviation: ACS Appl Bio Mater Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Washington, DC : ACS Publications, [2018]- |
| MeSH Terms: | Chitosan*/chemistry , Chitosan*/pharmacology , Hydrogels*/chemistry , Hydrogels*/pharmacology , Hydrogels*/chemical synthesis , Sulfhydryl Compounds*/chemistry , Sulfhydryl Compounds*/pharmacology , Biocompatible Materials*/chemistry , Biocompatible Materials*/pharmacology , Biocompatible Materials*/chemical synthesis , Stearic Acids*/chemistry , Stearic Acids*/pharmacology , Biomimetic Materials*/chemistry , Biomimetic Materials*/pharmacology , Biomimetic Materials*/chemical synthesis , Axons*/drug effects , Nerve Regeneration*/drug effects, Cell Proliferation/drug effects ; Neurons/drug effects ; Animals ; Materials Testing ; Mice ; Particle Size |
| Abstract: | Neuronal disorders like spinal cord injury, stroke, peripheral neuropathy, and neurodegenerative diseases like Alzheimer's disease, Parkinson's disease, etc., need regenerative approaches and therapeutics like tissue engineering and cell therapy for neuronal systems. Significant factors such as neuronal damage, impaired immune response, and glial scar formation create a hostile environment for neural repair and functional recovery. To overcome these challenges, the development of biocompatible and bioactive biomaterials is essential for therapeutic applications. In this work, a thiolated chitosan-stearic acid (Tch-Sa) hydrogel was prepared by modifying chitosan and stearic acid with free thiol (SH) groups, mimicking the high lipid content and GaGs in neuronal tissue. The hydrogel was crosslinked via disulfide linkages. The hydrogels were characterized using FTIR, 1H NMR, Raman spectroscopy, and TGA. Rheological evaluation demonstrated stable mechanical behavior, with storage and loss moduli showing structural consistency. In vitro cytocompatibility studies with N2a neuronal cells showed good adhesion, proliferation, and gradual morphological development. By day 7, cells displayed extended axonal projections. Immunofluorescence staining revealed increased expression of neuronal proteins, supporting the hydrogel's role in promoting neuronal differentiation and maturation. Moreover, the introduction of thiol groups enhanced cell permeability, mucoadhesive properties, and axonal outgrowth, suggesting that this material may be helpful in neuronal tissue engineering applications. |
| Contributed Indexing: | Keywords: hydrogel; neuronal cells; neuronal recovery; thiolated chitosan; thiolated stearic acid |
| Substance Nomenclature: | 9012-76-4 (Chitosan) 0 (Hydrogels) 0 (Sulfhydryl Compounds) 0 (Biocompatible Materials) 0 (Stearic Acids) 4ELV7Z65AP (stearic acid) |
| Entry Date(s): | Date Created: 20260608 Date Completed: 20260706 Latest Revision: 20260706 |
| Update Code: | 20260706 |
| DOI: | 10.1021/acsabm.5c02580 |
| PMID: | 42253176 |
| Database: | MEDLINE |
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