Academic Journal
PmPPO-mediated drought adaptation mechanism in Prunus mira Koehne: Interaction with PmRad23d and regulation of ROS homeostasis.
| Τίτλος: | PmPPO-mediated drought adaptation mechanism in Prunus mira Koehne: Interaction with PmRad23d and regulation of ROS homeostasis. |
|---|---|
| Συγγραφείς: | Li J; College of Horticulture, College of Forestry and Grassland Science, Jilin Provincial Key Laboratory of Tree and Grass Genetics and Breeding, Jilin Agricultural University, Changchun, 130118, China., Bao X; College of Science, Baicheng Normal University, Baicheng, 137099, China., Zhao Y; College of Science, Baicheng Normal University, Baicheng, 137099, China., Meng F; College of Horticulture, College of Forestry and Grassland Science, Jilin Provincial Key Laboratory of Tree and Grass Genetics and Breeding, Jilin Agricultural University, Changchun, 130118, China. Electronic address: mfjtougao@163.com. |
| Πηγή: | Plant physiology and biochemistry : PPB [Plant Physiol Biochem] 2026 Jul; Vol. 236, pp. 111458. Date of Electronic Publication: 2026 Jun 06. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Science Country of Publication: France NLM ID: 9882449 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2690 (Electronic) Linking ISSN: 09819428 NLM ISO Abbreviation: Plant Physiol Biochem Subsets: MEDLINE |
| Imprint Name(s): | Publication: Amsterdam : Elsevier Science Original Publication: Paris : Gauthier-Villars, c1987- |
| Ιατρικοί όροι (MeSH): | Catechol Oxidase*/metabolism , Catechol Oxidase*/genetics , Plant Proteins*/metabolism , Plant Proteins*/genetics , Prunus*/genetics , Prunus*/metabolism , Prunus*/physiology , Prunus*/enzymology , Reactive Oxygen Species*/metabolism , Drought Resistance*, Anthocyanins/metabolism ; Adaptation, Physiological ; Gene Expression Regulation, Plant ; Homeostasis ; Plants, Genetically Modified ; Stress, Physiological |
| Περίληψη: | Global warming has resulted in frequent droughts worldwide, significantly affecting plant normal growth and development. Polyphenol oxidase (PPO), a copper-containing redox enzyme in plants, serves multiple functions. However, limited research has investigated the role of PPO in regulating plant drought adaptation. In this study, PmPPO was isolated from Prunus mira Koehne, the wild ancestor of cultivated peaches, which is a rare tree species known for its high stress tolerance under extreme conditions. Our results indicated that PmPPO was preferentially expressed in young roots, with its expression significantly induced by drought and abscisic acid (ABA). Overexpression of PmPPO in plants demonstrated enhanced tolerance to drought stress. Under drought stress, transgenic plants exhibited increased antioxidant enzyme activity, improved reactive oxygen species (ROS) scavenging capacity, and reduced cellular damage compared to wild-type (WT). Moreover, PmPPO overexpression facilitated anthocyanin accumulation by regulating the expression of genes involved in anthocyanin biosynthesis following drought treatment. Additionally, yeast two-hybrid (Y2H) and Luciferase complementation imaging (LCI) assays confirmed interactions between PmPPO and both PmRad23d and PmRGLG2. These findings introduce new molecular targets for genetic manipulation in peach germplasm improvement and present potential candidate genes for screening stress tolerance through molecular breeding. (Copyright © 2026 The Authors. Published by Elsevier Masson SAS.. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Drought stress; PmPPO; PmRad23d; Prunus mira Koehne; ROS homeostasis |
| Substance Nomenclature: | 0 (Anthocyanins) EC 1.10.3.1 (Catechol Oxidase) 0 (Plant Proteins) 0 (Reactive Oxygen Species) |
| Entry Date(s): | Date Created: 20260607 Date Completed: 20260617 Latest Revision: 20260625 |
| Update Code: | 20260626 |
| DOI: | 10.1016/j.plaphy.2026.111458 |
| PMID: | 42251827 |
| Βάση Δεδομένων: | MEDLINE |
καταχωρήστε σχόλιο πρώτοι!