Academic Journal
Integrating molecular and physiological approaches to quantify genetic controls for wheat development and improve phenotyping.
| Τίτλος: | Integrating molecular and physiological approaches to quantify genetic controls for wheat development and improve phenotyping. |
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| Συγγραφείς: | Brown H; The New Zealand Institute for Bioeconomy Science, Christchurch 7608, New Zealand., McCallum J; The New Zealand Institute for Bioeconomy Science, Christchurch 7608, New Zealand.; Department of Biochemistry, University of Otago, Dunedin 9054, New Zealand., Johnston P; The New Zealand Institute for Bioeconomy Science, Christchurch 7608, New Zealand., Pither-Joyce M; The New Zealand Institute for Bioeconomy Science, Christchurch 7608, New Zealand., Macknight R; Department of Biochemistry, University of Otago, Dunedin 9054, New Zealand., Huth N; CSIRO Agriculture and Food, Forest Hill, Queensland 4342, Australia., Moot D; Faculty of Agriculture and Life Sciences, Lincoln University, Canterbury 7647, New Zealand., Zheng B; CSIRO Agriculture and Food, St Lucia, Queensland 4067, Australia., Zhao Z; CSIRO Agriculture and Food, Black Mountain, Australian Capital Territory 2601, Australia., Hunt J; School of Agriculture, Food and Ecosystem Sciences, University of Melbourne, Parkville, Victoria 3010, Australia., Wang E; CSIRO Agriculture and Food, Black Mountain, Australian Capital Territory 2601, Australia. |
| Πηγή: | Journal of experimental botany [J Exp Bot] 2026 Sep 16; Vol. 77 (17), pp. 5874-5889. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Oxford University Press Country of Publication: England NLM ID: 9882906 Publication Model: Print Cited Medium: Internet ISSN: 1460-2431 (Electronic) Linking ISSN: 00220957 NLM ISO Abbreviation: J Exp Bot Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Oxford, UK : Oxford University Press |
| Ιατρικοί όροι (MeSH): | Triticum*/genetics , Triticum*/growth & development , Triticum*/physiology , Plant Proteins*/genetics , Plant Proteins*/metabolism , Gene Expression Regulation, Plant*, Flowers/genetics ; Flowers/growth & development ; Flowers/physiology ; Plant Leaves/growth & development ; Plant Leaves/genetics ; Plant Leaves/physiology ; Phenotype ; Photoperiod ; Temperature ; Genotype ; Gene Expression Profiling |
| Περίληψη: | Disentangling genotype×environment (G×E) controls of flowering time requires phenotypes that link molecular regulation, developmental physiology, and environment. Here, we integrated time-resolved measurements of apical development, final leaf number, and expression of the flowering-time genes VRN1, VRN2, and VRN3 across contrasting temperature and photoperiod regimes in six wheat genotypes spanning a wide range of developmental sensitivities. By combining controlled-environment phenotyping with concurrent gene-expression profiling, we show that environmentally driven variation in final leaf number is coherently explained by shifts in the timing of key apical transitions and associated VRN gene-expression dynamics. These integrated datasets were used to parameterize and interrogate the Cereal Anthesis Molecular Phenology (CAMP) model, enabling direct comparison between observed foliar gene-expression time courses and modelled gene activity. While overall developmental responses were well captured by the model, systematic differences between observed and modelled gene-expression patterns highlight the importance of distinguishing foliar expression from apical regulatory activity, as well as differences in temporal scaling. Building on this framework, we present a phenotyping protocol based on final leaf number responses to defined temperature and photoperiod treatments that delivers unconfounded developmental phenotypes explicitly linked to underlying genetic regulation. (© The Author(s) 2026. Published by Oxford University Press on behalf of the Society for Experimental Biology.) |
| Competing Interests: | Conflict of interest: The authors declare they have no conflicts of interest. |
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| Grant Information: | New Zealand Institute for Plant and Food Research Capability Fund; ULA00011 Grains Research and Development Corporation |
| Contributed Indexing: | Keywords: Triticum aestivum; VRN genes; Final leaf number; flowering time; gene expression; phenology; photoperiod; vernalization |
| Substance Nomenclature: | 0 (Plant Proteins) |
| Entry Date(s): | Date Created: 20260606 Date Completed: 20260915 Latest Revision: 20260917 |
| Update Code: | 20260917 |
| PubMed Central ID: | PMC13577759 |
| DOI: | 10.1093/jxb/erag278 |
| PMID: | 42249571 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1460-2431 |
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| DOI: | 10.1093/jxb/erag278 |