Academic Journal
Green extraction of RuBisCO-rich leaf protein for sustainable food systems: process efficiency, functional quality, and sustainability assessment.
| Τίτλος: | Green extraction of RuBisCO-rich leaf protein for sustainable food systems: process efficiency, functional quality, and sustainability assessment. |
|---|---|
| Συγγραφείς: | Krishnan H; Department of Food Science and Nutrition, Amrita School of Physical Sciences, Coimbatore Amrita Vishwa Vidyapeetham, 641112, India., Lakshmipathy B; Department of Food Science and Nutrition, Amrita School of Physical Sciences, Coimbatore Amrita Vishwa Vidyapeetham, 641112, India. Electronic address: l_banupriya@cb.amrita.edu., Ramaswamy J; Department of Food Science and Nutrition, Amrita School of Physical Sciences, Coimbatore Amrita Vishwa Vidyapeetham, 641112, India., Nallamuthu I; Nutrition, Biochemistry and Toxicology Division, Defence Institute of Biodefence Technologies (Formerly Defence Food Research Laboratory), Mysore 570011, India., Dubey M; Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences, 75007 Uppsala, Sweden. Electronic address: Mukesh.Dubey@slu.se., Xu B; Food Science and Technology Program, Department of Life Sciences, BNU-HKBU United International College, Zhuhai, Guangdong 519087, China., Reddy CK; Department of Life Sciences, GITAM (Deemed to be University), Visakhapatnam 530045, India., Kumar M; Food Engineering Department, Agriculture Faculty, Atatürk University, 25240, Erzurum, Türkiye. |
| Πηγή: | Food chemistry [Food Chem] 2026 Aug 30; Vol. 521, pp. 149914. Date of Electronic Publication: 2026 Jun 02. |
| Τύπος έκδοσης: | Journal Article; Review |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Applied Science Publishers Country of Publication: England NLM ID: 7702639 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-7072 (Electronic) Linking ISSN: 03088146 NLM ISO Abbreviation: Food Chem Subsets: MEDLINE |
| Imprint Name(s): | Publication: Barking : Elsevier Applied Science Publishers Original Publication: Barking, Eng., Applied Science Publishers. |
| Ιατρικοί όροι (MeSH): | Plant Leaves*/chemistry , Plant Leaves*/enzymology , Ribulose-Bisphosphate Carboxylase*/chemistry , Ribulose-Bisphosphate Carboxylase*/isolation & purification , Ribulose-Bisphosphate Carboxylase*/analysis , Plant Proteins*/isolation & purification , Plant Proteins*/chemistry , Green Chemistry Technology*/methods , Plant Extracts*/isolation & purification , Plant Extracts*/chemistry |
| Περίληψη: | Leaf biomass, particularly ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO)-enriched fractions, represents a viable secondary source of protein for sustainable food systems; this review presents a focused, comparing green extraction approaches deep eutectic solvents, enzymatic pretreatments, ultrasound- and microwave-assisted methods, and hybrid combinations with respect to protein yield, purity and functional properties. Enzymatic pretreatment most consistently enhances protein recovery while preserving solubility and emulsification capacity; ultrasound and microwave assistance reduce processing time but require careful parameter control to avoid denaturation. Key barriers to scale-up include inconsistent reporting of performance metrics, solvent-recovery inefficiencies, limited pilot-scale validation, and gaps in techno-economic and safety data. Finally, a forward-looking roadmap proposed to overcome persistent challenges such as solvent recovery and the absence of standardized protocols, highlighting the promise of artificial intelligence (AI)-guided process optimization and tailored solvent design. Collectively, this review provides a distinct framework for advancing leaf protein valorization toward sustainable food system transformation. (Copyright © 2026 The Authors. Published by Elsevier Ltd.. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Circular bioeconomy; Deep eutectic solvents; Green extraction; Leaf protein; Life cycle assessment; RuBisCO; Sustainable food systems |
| Substance Nomenclature: | EC 4.1.1.39 (Ribulose-Bisphosphate Carboxylase) 0 (Plant Proteins) 0 (Plant Extracts) |
| Entry Date(s): | Date Created: 20260605 Date Completed: 20260703 Latest Revision: 20260703 |
| Update Code: | 20260703 |
| DOI: | 10.1016/j.foodchem.2026.149914 |
| PMID: | 42248027 |
| Βάση Δεδομένων: | MEDLINE |
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