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Denoising-Assisted Rapid Multiphoton Imaging for Analysing Traumatic Penumbra Microenvironment in Rat Brain.

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Τίτλος: Denoising-Assisted Rapid Multiphoton Imaging for Analysing Traumatic Penumbra Microenvironment in Rat Brain.
Συγγραφείς: Guo P; Chongqing Key Laboratory of Optical Fiber Sensor and Photoelectric Detection, Chongqing University of Technology, Chongqing, China., Chen L; Chongqing Key Laboratory of Optical Fiber Sensor and Photoelectric Detection, Chongqing University of Technology, Chongqing, China., Jiang S; Chongqing Key Laboratory of Optical Fiber Sensor and Photoelectric Detection, Chongqing University of Technology, Chongqing, China., Ai L; Postdoctoral Workstation, The Central Hospital Affiliated to Chongqing University of Technology, Chongqing University of Technology, Chongqing, China., Shi S; Chongqing Key Laboratory of Optical Fiber Sensor and Photoelectric Detection, Chongqing University of Technology, Chongqing, China., Yang D; Postdoctoral Workstation, The Central Hospital Affiliated to Chongqing University of Technology, Chongqing University of Technology, Chongqing, China., Xu J; Chaohu University, Chaohu, China., Chen J; Key Laboratory of Opto-Electronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Normal University, Fuzhou, China., Lu H; Postdoctoral Workstation, The Central Hospital Affiliated to Chongqing University of Technology, Chongqing University of Technology, Chongqing, China.
Πηγή: Journal of biophotonics [J Biophotonics] 2026 Jun; Vol. 19 (6), pp. e70303.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 101318567 Publication Model: Print Cited Medium: Internet ISSN: 1864-0648 (Electronic) Linking ISSN: 1864063X NLM ISO Abbreviation: J Biophotonics Subsets: MEDLINE
Imprint Name(s): Original Publication: Weinheim : Wiley-VCH
Ιατρικοί όροι (MeSH): Brain Injuries, Traumatic*/diagnostic imaging , Brain Injuries, Traumatic*/pathology , Microscopy, Fluorescence, Multiphoton*/methods , Image Processing, Computer-Assisted*/methods , Brain*/diagnostic imaging , Signal-To-Noise Ratio* , Cellular Microenvironment*, Animals ; Rats ; Time Factors ; Rats, Sprague-Dawley ; Male
Περίληψη: Accurate identification of the traumatic penumbra (TP) is essential for understanding secondary injury after traumatic brain injury. However, conventional imaging cannot capture microenvironmental alterations at subcellular resolution. In this study, denoising-assisted multiphoton microscopy (MPM) was used to investigate the microstructural features of the TP in rat brain tissue. Image processing and quantitative analysis were performed to characterize normal tissue, penumbra, and core regions. To accelerate high-quality imaging, we proposed a Dual-Branch Collaborative Network (DBCNet) to restore low-noise images from single-scan data using multi-scan references. The results show that MPM reveals key pathological features such as intracellular edema, vasogenic edema, and cytoplasmic matrix disruption. DBCNet effectively suppresses noise, enhances fine structures, and reduces imaging time to one-fourth without hardware modification. These findings demonstrate that denoising-assisted MPM provides a rapid and efficient strategy for high-resolution imaging and quantitative assessment of the TP microenvironment.
(© 2026 Wiley‐VCH GmbH.)
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Grant Information: 2023BM07 the Anhui Provincial Engineering Technology Research Center for Biomedical Instrument; 2024AH051341 the Natural Science Foundation of the Higher Education Institutions of Anhui Province; KJQN202501117 the Science and Technology Research Program of Chongqing Municipal Education Commission; KJZD-K202301105 the Science and Technology Research Program of Chongqing Municipal Education Commission
Contributed Indexing: Keywords: collaborative network; image denoising; multiphoton imaging; traumatic penumbra
Entry Date(s): Date Created: 20260602 Date Completed: 20260607 Latest Revision: 20260607
Update Code: 20260607
DOI: 10.1002/jbio.70303
PMID: 42227888
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:1864-0648
DOI:10.1002/jbio.70303