Academic Journal
Enhancing Soluble Expression and Post-Translational Modification of Mussel Foot Protein in E. coli.
| Τίτλος: | Enhancing Soluble Expression and Post-Translational Modification of Mussel Foot Protein in E. coli. |
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| Συγγραφείς: | Zhang R; School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, P. R. China.; Institute of Future Food Technology, JITRI, Yixing 214200, P. R. China., Xue D; School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, P. R. China.; Wuxi Children's Hospital, Wuxi 214023, P. R. China., Chen JP; School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, P. R. China.; Institute of Future Food Technology, JITRI, Yixing 214200, P. R. China., Su C; School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, P. R. China.; Institute of Future Food Technology, JITRI, Yixing 214200, P. R. China., Li H; School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, P. R. China., Gong JS; School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, P. R. China.; Institute of Future Food Technology, JITRI, Yixing 214200, P. R. China., Xu ZH; National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, School of Biotechnology, Jiangnan University, Wuxi 214122, P. R. China.; College of Biomass Science and Engineering, Sichuan University, Chengdu 610065, P. R. China.; Institute of Future Food Technology, JITRI, Yixing 214200, P. R. China., Shi JS; School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, P. R. China.; Institute of Future Food Technology, JITRI, Yixing 214200, P. R. China. |
| Πηγή: | Journal of agricultural and food chemistry [J Agric Food Chem] 2026 Jun 17; Vol. 74 (23), pp. 18179-18188. Date of Electronic Publication: 2026 Jun 02. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 0374755 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-5118 (Electronic) Linking ISSN: 00218561 NLM ISO Abbreviation: J Agric Food Chem Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Washington, American Chemical Society. |
| Ιατρικοί όροι (MeSH): | Escherichia coli*/genetics , Escherichia coli*/metabolism , Proteins*/genetics , Proteins*/chemistry , Proteins*/metabolism , Bivalvia*/genetics , Bivalvia*/metabolism , Bivalvia*/chemistry, Recombinant Proteins/chemistry ; Recombinant Proteins/genetics ; Recombinant Proteins/metabolism ; Monophenol Monooxygenase/metabolism ; Monophenol Monooxygenase/genetics ; Animals ; Solubility ; Protein Processing, Post-Translational ; Gene Expression |
| Περίληψη: | Due to intrinsically low solubility and stability, recombinant mussel foot proteins (Mfps) require an efficient system that supports large-scale production of dopanized-Mfps. Here, we engineered a 39-aa peptide tag, termed T7B9M3, to increase the solubility of Mfps in Escherichia coli. The absence of net charged residues, rather than the copy number or the predicted α-helical structure of the T7B9M3 tag, contributes to the solubility enhancement. T7B9M3-fp-3B achieved a 365.96 mg/L soluble protein titer with a 98.75% soluble rate in a shake flask. Coupling the tyrosinase coexpression to endow Mfps with a dopaminization capability, the tyrosine hydroxylation efficiency reached 19.82%. Moreover, dopanized-Mfps tended to form stronger interactions with various substrates, including glass, polypropylene, and polyethylene, than the unmodified version. Our findings suggest that the combined strategy may contribute to establishing a scalable and low-cost protein expression platform with the potential for batch production of functional Mfps. |
| Contributed Indexing: | Keywords: adhesion performance; mussel foot protein; recombinant fp-3B; soluble expression; tyrosinase |
| Substance Nomenclature: | 0 (Recombinant Proteins) EC 1.14.18.1 (Monophenol Monooxygenase) 0 (Proteins) |
| Entry Date(s): | Date Created: 20260602 Date Completed: 20260617 Latest Revision: 20260617 |
| Update Code: | 20260617 |
| DOI: | 10.1021/acs.jafc.6c03491 |
| PMID: | 42227823 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1520-5118 |
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| DOI: | 10.1021/acs.jafc.6c03491 |