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Metabacillus harenarius sp. nov., an alginate lyase-producing bacterium, and reclassification of 'Bacillus weihaiensis'.

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Τίτλος: Metabacillus harenarius sp. nov., an alginate lyase-producing bacterium, and reclassification of 'Bacillus weihaiensis'.
Συγγραφείς: Liang R; Guangxi Key Laboratory for Polysaccharide Materials and Modifications, School of Marine Sciences and Biotechnology, Guangxi Minzu University, Nanning 530006, PR China.; Institute of Tropical Bioscience and Biotechnology & Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101/Sanya 572024, PR China., Mo K; Institute of Tropical Bioscience and Biotechnology & Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101/Sanya 572024, PR China.; Hainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bio-resources, Haikou 571101, PR China., Zhao Z; Guangxi Key Laboratory for Polysaccharide Materials and Modifications, School of Marine Sciences and Biotechnology, Guangxi Minzu University, Nanning 530006, PR China.; Institute of Tropical Bioscience and Biotechnology & Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101/Sanya 572024, PR China., Hu Y; Institute of Tropical Bioscience and Biotechnology & Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101/Sanya 572024, PR China.; Hainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bio-resources, Haikou 571101, PR China.; Zhanjiang Experimental Station, CATAS, Zhanjiang, 524013, PR China., Zhang Z; Guangxi Key Laboratory for Polysaccharide Materials and Modifications, School of Marine Sciences and Biotechnology, Guangxi Minzu University, Nanning 530006, PR China., Jiang M; Guangxi Key Laboratory for Polysaccharide Materials and Modifications, School of Marine Sciences and Biotechnology, Guangxi Minzu University, Nanning 530006, PR China., Huang H; Institute of Tropical Bioscience and Biotechnology & Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101/Sanya 572024, PR China.; Hainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bio-resources, Haikou 571101, PR China.
Πηγή: International journal of systematic and evolutionary microbiology [Int J Syst Evol Microbiol] 2026 Jun; Vol. 76 (6).
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Microbiology Society Country of Publication: England NLM ID: 100899600 Publication Model: Print Cited Medium: Internet ISSN: 1466-5034 (Electronic) Linking ISSN: 14665026 NLM ISO Abbreviation: Int J Syst Evol Microbiol Subsets: MEDLINE
Imprint Name(s): Publication: 2015- : London : Microbiology Society
Original Publication: Reading, UK : Society for General Microbiology, c2000-
Ιατρικοί όροι (MeSH): Polysaccharide-Lyases*/metabolism , Bacillaceae*/classification , Bacillaceae*/genetics , Bacillaceae*/isolation & purification , Phylogeny* , Soil Microbiology*, RNA, Ribosomal, 16S/genetics ; Fatty Acids/chemistry ; DNA, Bacterial/genetics ; Phospholipids/chemistry ; Vitamin K 2/analogs & derivatives ; Vitamin K 2/chemistry ; Sand/microbiology ; Bacillus/classification ; Bacillus/genetics ; Bacterial Typing Techniques ; Base Composition ; Sequence Analysis, DNA ; China ; Nucleic Acid Hybridization
Περίληψη: A Gram-stain-negative, motile, rod-shaped, alginate lyase-producing bacterium, designated HB246100T, was isolated from coastal sand sample collected from Luhuitou Scenic Area, Sanya, China. The strain grew at 15-50 ℃ (optimum, 35 ℃), pH 6.0-11.0 (optimal, pH 8.0) and 0-10% (w/v) NaCl (optimum, 3%). The predominant isoprenoid quinone was found to be menaquinone 7, and the major fatty acids were anteiso-C15 : 0, iso-C15 : 0, iso-C14 : 0 and iso-C16 : 0. The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, an unidentified aminophospholipid, an unidentified lipid, three unidentified phospholipids and three unidentified glycolipids. The size of the genome was 4.29 Mbp, with a G+C content of 36.5 mol%. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain HB246100T belonged to the genus Metabacillus, and the closest phylogenetically related type species were Metabacillus crassostreae JSM 100118T, Metabacillus schmidteae Marseille-P9898T, Metabacillus halosaccharovorans E33T and Metabacillus litoralis SW-211T with the identities of 98.9, 98.1, 98.0 and 98.0%, respectively. The phylogenetic tree based on 16S rRNA gene sequences and the phylogenomic tree based on core genome sequences indicated that the novel strain was most closely related to M. crassostreae and M. litoralis, with average nucleotide identity of 74.8-75.6% and digital DNA-DNA hybridization value of 22.1-22.4%. Comprehensive phenotypic, phylogenetic and genomic characteristics demonstrate that strain HB246100T is distinct from the species with validly published names in the genus Metabacillus, suggesting that it represents a novel species of the genus Metabacillus and proposing the name Metabacillus harenarius sp. nov. The type strain is HB246100T (=MCCC 1K09923T=JCM 38130T). Furthermore, 'Bacillus weihaiensis' Alg07, which only publicly disclosed its genome sequence, was emended and reclassified as M. harenarius.
Contributed Indexing: Keywords: 'Bacillus weihaiensis'; Metabacillus harenarius sp. nov.; alginate lyase; coastal sand
Substance Nomenclature: 0 (RNA, Ribosomal, 16S)
0 (Fatty Acids)
EC 4.2.2.- (Polysaccharide-Lyases)
0 (DNA, Bacterial)
EC 4.2.2.3 (poly(beta-D-mannuronate) lyase)
0 (Phospholipids)
11032-49-8 (Vitamin K 2)
0 (Sand)
8427BML8NY (menaquinone 7)
Entry Date(s): Date Created: 20260601 Date Completed: 20260717 Latest Revision: 20260717
Update Code: 20260717
DOI: 10.1099/ijsem.0.007185
PMID: 42223994
Βάση Δεδομένων: MEDLINE
FullText Text:
  Availability: 0
Header DbId: cmedm
DbLabel: MEDLINE
An: 42223994
AccessLevel: 3
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Metabacillus harenarius sp. nov., an alginate lyase-producing bacterium, and reclassification of 'Bacillus weihaiensis'.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AU" term="%22Liang+R%22">Liang R</searchLink>; Guangxi Key Laboratory for Polysaccharide Materials and Modifications, School of Marine Sciences and Biotechnology, Guangxi Minzu University, Nanning 530006, PR China.; Institute of Tropical Bioscience and Biotechnology & Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101/Sanya 572024, PR China.<br /><searchLink fieldCode="AU" term="%22Mo+K%22">Mo K</searchLink>; Institute of Tropical Bioscience and Biotechnology & Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101/Sanya 572024, PR China.; Hainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bio-resources, Haikou 571101, PR China.<br /><searchLink fieldCode="AU" term="%22Zhao+Z%22">Zhao Z</searchLink>; Guangxi Key Laboratory for Polysaccharide Materials and Modifications, School of Marine Sciences and Biotechnology, Guangxi Minzu University, Nanning 530006, PR China.; Institute of Tropical Bioscience and Biotechnology & Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101/Sanya 572024, PR China.<br /><searchLink fieldCode="AU" term="%22Hu+Y%22">Hu Y</searchLink>; Institute of Tropical Bioscience and Biotechnology & Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101/Sanya 572024, PR China.; Hainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bio-resources, Haikou 571101, PR China.; Zhanjiang Experimental Station, CATAS, Zhanjiang, 524013, PR China.<br /><searchLink fieldCode="AU" term="%22Zhang+Z%22">Zhang Z</searchLink>; Guangxi Key Laboratory for Polysaccharide Materials and Modifications, School of Marine Sciences and Biotechnology, Guangxi Minzu University, Nanning 530006, PR China.<br /><searchLink fieldCode="AU" term="%22Jiang+M%22">Jiang M</searchLink>; Guangxi Key Laboratory for Polysaccharide Materials and Modifications, School of Marine Sciences and Biotechnology, Guangxi Minzu University, Nanning 530006, PR China.<br /><searchLink fieldCode="AU" term="%22Huang+H%22">Huang H</searchLink>; Institute of Tropical Bioscience and Biotechnology & Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101/Sanya 572024, PR China.; Hainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bio-resources, Haikou 571101, PR China.
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22100899600%22">International journal of systematic and evolutionary microbiology</searchLink> [Int J Syst Evol Microbiol] 2026 Jun; Vol. 76 (6).
– Name: TypePub
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  Group: TypPub
  Data: Journal Article
– Name: Language
  Label: Language
  Group: Lang
  Data: English
– Name: TitleSource
  Label: Journal Info
  Group: Src
  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22Microbiology+Society%22">Microbiology Society </searchLink><i>Country of Publication: </i>England <i>NLM ID: </i>100899600 <i>Publication Model: </i>Print <i>Cited Medium: </i>Internet <i>ISSN: </i>1466-5034 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2214665026%22">14665026 </searchLink><i>NLM ISO Abbreviation: </i>Int J Syst Evol Microbiol <i>Subsets: </i>MEDLINE
– Name: PublisherInfo
  Label: Imprint Name(s)
  Group: PubInfo
  Data: <i>Publication</i>: 2015- : London : Microbiology Society<br /><i>Original Publication</i>: Reading, UK : Society for General Microbiology, c2000-
– Name: SubjectMESH
  Label: MeSH Terms
  Group: Su
  Data: <searchLink fieldCode="MM" term="%22Polysaccharide-Lyases%22">Polysaccharide-Lyases*</searchLink>/<searchLink fieldCode="MM" term="%22Polysaccharide-Lyases+metabolism%22">metabolism</searchLink> <br /><searchLink fieldCode="MM" term="%22Bacillaceae%22">Bacillaceae*</searchLink>/<searchLink fieldCode="MM" term="%22Bacillaceae+classification%22">classification</searchLink> <br /><searchLink fieldCode="MM" term="%22Bacillaceae%22">Bacillaceae*</searchLink>/<searchLink fieldCode="MM" term="%22Bacillaceae+genetics%22">genetics</searchLink> <br /><searchLink fieldCode="MM" term="%22Bacillaceae%22">Bacillaceae*</searchLink>/<searchLink fieldCode="MM" term="%22Bacillaceae+isolation+%26+purification%22">isolation & purification</searchLink> <br /><searchLink fieldCode="MM" term="%22Phylogeny%22">Phylogeny*</searchLink> <br /><searchLink fieldCode="MM" term="%22Soil+Microbiology%22">Soil Microbiology*</searchLink><br /><searchLink fieldCode="MH" term="%22RNA%2C+Ribosomal%2C+16S%22">RNA, Ribosomal, 16S</searchLink>/<searchLink fieldCode="MH" term="%22RNA%2C+Ribosomal%2C+16S+genetics%22">genetics</searchLink> ; <searchLink fieldCode="MH" term="%22Fatty+Acids%22">Fatty Acids</searchLink>/<searchLink fieldCode="MH" term="%22Fatty+Acids+chemistry%22">chemistry</searchLink> ; <searchLink fieldCode="MH" term="%22DNA%2C+Bacterial%22">DNA, Bacterial</searchLink>/<searchLink fieldCode="MH" term="%22DNA%2C+Bacterial+genetics%22">genetics</searchLink> ; <searchLink fieldCode="MH" term="%22Phospholipids%22">Phospholipids</searchLink>/<searchLink fieldCode="MH" term="%22Phospholipids+chemistry%22">chemistry</searchLink> ; <searchLink fieldCode="MH" term="%22Vitamin+K+2%22">Vitamin K 2</searchLink>/<searchLink fieldCode="MH" term="%22Vitamin+K+2+analogs+%26+derivatives%22">analogs & derivatives</searchLink> ; <searchLink fieldCode="MH" term="%22Vitamin+K+2%22">Vitamin K 2</searchLink>/<searchLink fieldCode="MH" term="%22Vitamin+K+2+chemistry%22">chemistry</searchLink> ; <searchLink fieldCode="MH" term="%22Sand%22">Sand</searchLink>/<searchLink fieldCode="MH" term="%22Sand+microbiology%22">microbiology</searchLink> ; <searchLink fieldCode="MH" term="%22Bacillus%22">Bacillus</searchLink>/<searchLink fieldCode="MH" term="%22Bacillus+classification%22">classification</searchLink> ; <searchLink fieldCode="MH" term="%22Bacillus%22">Bacillus</searchLink>/<searchLink fieldCode="MH" term="%22Bacillus+genetics%22">genetics</searchLink> ; <searchLink fieldCode="MH" term="%22Bacterial+Typing+Techniques%22">Bacterial Typing Techniques</searchLink> ; <searchLink fieldCode="MH" term="%22Base+Composition%22">Base Composition</searchLink> ; <searchLink fieldCode="MH" term="%22Sequence+Analysis%2C+DNA%22">Sequence Analysis, DNA</searchLink> ; <searchLink fieldCode="MH" term="%22China%22">China</searchLink> ; <searchLink fieldCode="MH" term="%22Nucleic+Acid+Hybridization%22">Nucleic Acid Hybridization</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: A Gram-stain-negative, motile, rod-shaped, alginate lyase-producing bacterium, designated HB246100<superscript>T</superscript>, was isolated from coastal sand sample collected from Luhuitou Scenic Area, Sanya, China. The strain grew at 15-50 ℃ (optimum, 35 ℃), pH 6.0-11.0 (optimal, pH 8.0) and 0-10% (w/v) NaCl (optimum, 3%). The predominant isoprenoid quinone was found to be menaquinone 7, and the major fatty acids were anteiso-C<subscript>15 : 0</subscript>, iso-C<subscript>15 : 0</subscript>, iso-C<subscript>14 : 0</subscript> and iso-C<subscript>16 : 0</subscript>. The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, an unidentified aminophospholipid, an unidentified lipid, three unidentified phospholipids and three unidentified glycolipids. The size of the genome was 4.29 Mbp, with a G+C content of 36.5 mol%. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain HB246100<superscript>T</superscript> belonged to the genus Metabacillus, and the closest phylogenetically related type species were Metabacillus crassostreae JSM 100118<superscript>T</superscript>, Metabacillus schmidteae Marseille-P9898<superscript>T</superscript>, Metabacillus halosaccharovorans E33<superscript>T</superscript> and Metabacillus litoralis SW-211<superscript>T</superscript> with the identities of 98.9, 98.1, 98.0 and 98.0%, respectively. The phylogenetic tree based on 16S rRNA gene sequences and the phylogenomic tree based on core genome sequences indicated that the novel strain was most closely related to M. crassostreae and M. litoralis, with average nucleotide identity of 74.8-75.6% and digital DNA-DNA hybridization value of 22.1-22.4%. Comprehensive phenotypic, phylogenetic and genomic characteristics demonstrate that strain HB246100<superscript>T</superscript> is distinct from the species with validly published names in the genus Metabacillus, suggesting that it represents a novel species of the genus Metabacillus and proposing the name Metabacillus harenarius sp. nov. The type strain is HB246100<superscript>T</superscript> (=MCCC 1K09923<superscript>T</superscript>=JCM 38130<superscript>T</superscript>). Furthermore, 'Bacillus weihaiensis' Alg07, which only publicly disclosed its genome sequence, was emended and reclassified as M. harenarius.
– Name: SubjectMinor
  Label: Contributed Indexing
  Group:
  Data: <i>Keywords: </i>'Bacillus weihaiensis'; Metabacillus harenarius sp. nov.; alginate lyase; coastal sand
– Name: NumberCAS
  Label: Substance Nomenclature
  Group: ID
  Data: 0 (RNA, Ribosomal, 16S)<br />0 (Fatty Acids)<br />EC 4.2.2.- (Polysaccharide-Lyases)<br />0 (DNA, Bacterial)<br />EC 4.2.2.3 (poly(beta-D-mannuronate) lyase)<br />0 (Phospholipids)<br />11032-49-8 (Vitamin K 2)<br />0 (Sand)<br />8427BML8NY (menaquinone 7)
– Name: DateEntry
  Label: Entry Date(s)
  Group: Date
  Data: <i>Date Created: </i>20260601 <i>Date Completed: </i>20260717 <i>Latest Revision: </i>20260717
– Name: DateUpdate
  Label: Update Code
  Group: Date
  Data: 20260717
– Name: DOI
  Label: DOI
  Group: ID
  Data: 10.1099/ijsem.0.007185
– Name: AN
  Label: PMID
  Group: ID
  Data: 42223994
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=cmedm&AN=42223994
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        Value: 10.1099/ijsem.0.007185
    Languages:
      – Code: eng
        Text: English
    Subjects:
      – SubjectFull: RNA, Ribosomal, 16S genetics
        Type: general
      – SubjectFull: Fatty Acids chemistry
        Type: general
      – SubjectFull: DNA, Bacterial genetics
        Type: general
      – SubjectFull: Phospholipids chemistry
        Type: general
      – SubjectFull: Vitamin K 2 analogs & derivatives
        Type: general
      – SubjectFull: Vitamin K 2 chemistry
        Type: general
      – SubjectFull: Sand microbiology
        Type: general
      – SubjectFull: Bacillus classification
        Type: general
      – SubjectFull: Bacillus genetics
        Type: general
      – SubjectFull: Bacterial Typing Techniques
        Type: general
      – SubjectFull: Base Composition
        Type: general
      – SubjectFull: Sequence Analysis, DNA
        Type: general
      – SubjectFull: China
        Type: general
      – SubjectFull: Nucleic Acid Hybridization
        Type: general
      – SubjectFull: Polysaccharide-Lyases metabolism
        Type: general
      – SubjectFull: Bacillaceae classification
        Type: general
      – SubjectFull: Bacillaceae genetics
        Type: general
      – SubjectFull: Bacillaceae isolation & purification
        Type: general
      – SubjectFull: Phylogeny
        Type: general
      – SubjectFull: Soil Microbiology
        Type: general
    Titles:
      – TitleFull: Metabacillus harenarius sp. nov., an alginate lyase-producing bacterium, and reclassification of 'Bacillus weihaiensis'.
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              Text: 2026 Jun
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